Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions

Abstract Chamaecyparis taiwanensis is an endemic plant suffering illegal logging in Taiwan for its high economic value. Lack of direct evidence to correlate stump and timber remains a hurdle for law enforcement. In this report, 23 polymorphic Genomic Simple Sequence Repeat (gSSR) and 12 Expressed Se...

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Autores principales:	Chiun-Jr Huang, Fang-Hua Chu, Yi-Shiang Huang, Yu- Mei Hung, Yu-Hsin Tseng, Chang-En Pu, Chi-Hsiang Chao, Yu-Shyang Chou, Shau-Chian Liu, Ya Ting You, Shuo-Yu Hsu, Hsiang-Chih Hsieh, Cheng Te Hsu, Meng-Yi Chen, Ting-An Lin, Hsin-Yi Shyu, Yu-Ching Tu, Chi-Tsong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c7505c0535c42fb8229656a0dba3d5e
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spelling	oai:doaj.org-article:4c7505c0535c42fb8229656a0dba3d5e2021-12-02T13:58:10ZDevelopment and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions10.1038/s41598-020-79061-z2045-2322https://doaj.org/article/4c7505c0535c42fb8229656a0dba3d5e2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79061-zhttps://doaj.org/toc/2045-2322Abstract Chamaecyparis taiwanensis is an endemic plant suffering illegal logging in Taiwan for its high economic value. Lack of direct evidence to correlate stump and timber remains a hurdle for law enforcement. In this report, 23 polymorphic Genomic Simple Sequence Repeat (gSSR) and 12 Expressed Sequence Tag (EST)-SSR markers were developed and their transferability was assessed. The individual identification system built from selected non-linkage 30 SSR markers has a combined probability of identity as 5.596 × 10–12 equivalents to identifying an individual in a population of up to 18 million C. taiwanensis with 99.99% confidence level. We also applied the system in an actual criminal case by selecting 19 of these markers to correlate illegally felled timbers and victim trees. Our data demonstrate that molecular signals from three timbers hit with three victim trees with confidence level more than 99.99%. This is the first example of successfully applying SSR in C. taiwanensis as a court evidence for law enforcement. The identification system adapted advanced molecular technology and exhibits its great potential for natural resource management on C. taiwanensis.Chiun-Jr HuangFang-Hua ChuYi-Shiang HuangYu- Mei HungYu-Hsin TsengChang-En PuChi-Hsiang ChaoYu-Shyang ChouShau-Chian LiuYa Ting YouShuo-Yu HsuHsiang-Chih HsiehCheng Te HsuMeng-Yi ChenTing-An LinHsin-Yi ShyuYu-Ching TuChi-Tsong ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chiun-Jr Huang Fang-Hua Chu Yi-Shiang Huang Yu- Mei Hung Yu-Hsin Tseng Chang-En Pu Chi-Hsiang Chao Yu-Shyang Chou Shau-Chian Liu Ya Ting You Shuo-Yu Hsu Hsiang-Chih Hsieh Cheng Te Hsu Meng-Yi Chen Ting-An Lin Hsin-Yi Shyu Yu-Ching Tu Chi-Tsong Chen Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions
description	Abstract Chamaecyparis taiwanensis is an endemic plant suffering illegal logging in Taiwan for its high economic value. Lack of direct evidence to correlate stump and timber remains a hurdle for law enforcement. In this report, 23 polymorphic Genomic Simple Sequence Repeat (gSSR) and 12 Expressed Sequence Tag (EST)-SSR markers were developed and their transferability was assessed. The individual identification system built from selected non-linkage 30 SSR markers has a combined probability of identity as 5.596 × 10–12 equivalents to identifying an individual in a population of up to 18 million C. taiwanensis with 99.99% confidence level. We also applied the system in an actual criminal case by selecting 19 of these markers to correlate illegally felled timbers and victim trees. Our data demonstrate that molecular signals from three timbers hit with three victim trees with confidence level more than 99.99%. This is the first example of successfully applying SSR in C. taiwanensis as a court evidence for law enforcement. The identification system adapted advanced molecular technology and exhibits its great potential for natural resource management on C. taiwanensis.
format	article
author	Chiun-Jr Huang Fang-Hua Chu Yi-Shiang Huang Yu- Mei Hung Yu-Hsin Tseng Chang-En Pu Chi-Hsiang Chao Yu-Shyang Chou Shau-Chian Liu Ya Ting You Shuo-Yu Hsu Hsiang-Chih Hsieh Cheng Te Hsu Meng-Yi Chen Ting-An Lin Hsin-Yi Shyu Yu-Ching Tu Chi-Tsong Chen
author_facet	Chiun-Jr Huang Fang-Hua Chu Yi-Shiang Huang Yu- Mei Hung Yu-Hsin Tseng Chang-En Pu Chi-Hsiang Chao Yu-Shyang Chou Shau-Chian Liu Ya Ting You Shuo-Yu Hsu Hsiang-Chih Hsieh Cheng Te Hsu Meng-Yi Chen Ting-An Lin Hsin-Yi Shyu Yu-Ching Tu Chi-Tsong Chen
author_sort	Chiun-Jr Huang
title	Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions
title_short	Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions
title_full	Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions
title_fullStr	Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions
title_full_unstemmed	Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions
title_sort	development and technical application of ssr-based individual identification system for chamaecyparis taiwanensis against illegal logging convictions
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4c7505c0535c42fb8229656a0dba3d5e
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Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions

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