Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features

We report on a major update to the animal rDNA loci database, which now contains cytogenetic information for 45S and 5S rDNA loci in more than 2600 and 1000 species, respectively.<strong> </strong>The data analyses show the following: (i) A high variability in 5S and 45S loci numbers, wi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jana Sochorová, Francisco Gálvez, Roman Matyášek, Sònia Garcia, Aleš Kovařík
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ribosomal DNA rDNA rRNA genes nucleolar organizer regions karyotype sex chromosome Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4c75c6ac7dcf473baf9313f8ce8b3dc2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4c75c6ac7dcf473baf9313f8ce8b3dc2
record_format	dspace
spelling	oai:doaj.org-article:4c75c6ac7dcf473baf9313f8ce8b3dc22021-11-11T16:52:42ZAnalyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features10.3390/ijms2221114031422-00671661-6596https://doaj.org/article/4c75c6ac7dcf473baf9313f8ce8b3dc22021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11403https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067We report on a major update to the animal rDNA loci database, which now contains cytogenetic information for 45S and 5S rDNA loci in more than 2600 and 1000 species, respectively.<strong> </strong>The data analyses show the following: (i) A high variability in 5S and 45S loci numbers, with both showing 50-fold or higher variability. However, karyotypes with an extremely high number of loci were rare, and medians generally converged to two 5S sites and two 45S rDNA sites per diploid genome. No relationship was observed between the number of 5S and 45S loci. (ii) The position of 45S rDNA on sex chromosomes was relatively frequent in some groups, particularly in arthropods (14% of karyotypes). Furthermore, 45S rDNA was almost exclusively located in microchromosomes when these were present (in birds and reptiles). (iii) The proportion of active NORs (positively stained with silver staining methods) progressively decreased with an increasing number of 45S rDNA loci, and karyotypes with more than 12 loci showed, on average, less than 40% of active loci. In conclusion, the updated version of the database provides some new insights into the organization of rRNA genes in chromosomes. We expect that its updated content will be useful for taxonomists, comparative cytogeneticists, and evolutionary biologists. Jana SochorováFrancisco GálvezRoman MatyášekSònia GarciaAleš KovaříkMDPI AGarticleribosomal DNArDNArRNA genesnucleolar organizer regionskaryotypesex chromosomeBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11403, p 11403 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ribosomal DNA rDNA rRNA genes nucleolar organizer regions karyotype sex chromosome Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ribosomal DNA rDNA rRNA genes nucleolar organizer regions karyotype sex chromosome Biology (General) QH301-705.5 Chemistry QD1-999 Jana Sochorová Francisco Gálvez Roman Matyášek Sònia Garcia Aleš Kovařík Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features
description	We report on a major update to the animal rDNA loci database, which now contains cytogenetic information for 45S and 5S rDNA loci in more than 2600 and 1000 species, respectively.<strong> </strong>The data analyses show the following: (i) A high variability in 5S and 45S loci numbers, with both showing 50-fold or higher variability. However, karyotypes with an extremely high number of loci were rare, and medians generally converged to two 5S sites and two 45S rDNA sites per diploid genome. No relationship was observed between the number of 5S and 45S loci. (ii) The position of 45S rDNA on sex chromosomes was relatively frequent in some groups, particularly in arthropods (14% of karyotypes). Furthermore, 45S rDNA was almost exclusively located in microchromosomes when these were present (in birds and reptiles). (iii) The proportion of active NORs (positively stained with silver staining methods) progressively decreased with an increasing number of 45S rDNA loci, and karyotypes with more than 12 loci showed, on average, less than 40% of active loci. In conclusion, the updated version of the database provides some new insights into the organization of rRNA genes in chromosomes. We expect that its updated content will be useful for taxonomists, comparative cytogeneticists, and evolutionary biologists.
format	article
author	Jana Sochorová Francisco Gálvez Roman Matyášek Sònia Garcia Aleš Kovařík
author_facet	Jana Sochorová Francisco Gálvez Roman Matyášek Sònia Garcia Aleš Kovařík
author_sort	Jana Sochorová
title	Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features
title_short	Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features
title_full	Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features
title_fullStr	Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features
title_full_unstemmed	Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features
title_sort	analyses of the updated “animal rdna loci database” with an emphasis on its new features
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c75c6ac7dcf473baf9313f8ce8b3dc2
work_keys_str_mv	AT janasochorova analysesoftheupdatedanimalrdnalocidatabasewithanemphasisonitsnewfeatures AT franciscogalvez analysesoftheupdatedanimalrdnalocidatabasewithanemphasisonitsnewfeatures AT romanmatyasek analysesoftheupdatedanimalrdnalocidatabasewithanemphasisonitsnewfeatures AT soniagarcia analysesoftheupdatedanimalrdnalocidatabasewithanemphasisonitsnewfeatures AT aleskovarik analysesoftheupdatedanimalrdnalocidatabasewithanemphasisonitsnewfeatures
_version_	1718432236251906048

Analyses of the Updated “Animal rDNA Loci Database” with an Emphasis on Its New Features

Ejemplares similares