Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme

Abstract Neural crest cells (NCCs) give rise to various tissues including neurons, pigment cells, bone and cartilage in the head. Distal-less homeobox 5 (Dlx5) is involved in both jaw patterning and differentiation of NCC-derivatives. In this study, we investigated the differentiation potential of h...

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Autores principales:	Tri H. Vu, Masaki Takechi, Miki Shimizu, Taro Kitazawa, Hiroki Higashiyama, Akiyasu Iwase, Hiroki Kurihara, Sachiko Iseki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c786c597a874c7aa20414f54f069d87
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spelling	oai:doaj.org-article:4c786c597a874c7aa20414f54f069d872021-12-02T13:51:06ZDlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme10.1038/s41598-021-81434-x2045-2322https://doaj.org/article/4c786c597a874c7aa20414f54f069d872021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81434-xhttps://doaj.org/toc/2045-2322Abstract Neural crest cells (NCCs) give rise to various tissues including neurons, pigment cells, bone and cartilage in the head. Distal-less homeobox 5 (Dlx5) is involved in both jaw patterning and differentiation of NCC-derivatives. In this study, we investigated the differentiation potential of head mesenchyme by forcing Dlx5 to be expressed in mouse NCC (NCC Dlx5 ). In NCC Dlx5 mice, differentiation of dermis and pigment cells were enhanced with ectopic cartilage (ec) and heterotopic bone (hb) in different layers at the cranial vertex. The ec and hb were derived from the early migrating mesenchyme (EMM), the non-skeletogenic cell population located above skeletogenic supraorbital mesenchyme (SOM). The ec developed within Foxc1 +-dura mater with increased PDGFRα signalling, and the hb formed with upregulation of BMP and WNT/β-catenin signallings in Dermo1 +-dermal layer from E11.5. Since dermal cells express Runx2 and Msx2 in the control, osteogenic potential in dermal cells seemed to be inhibited by an anti-osteogenic function of Msx2 in normal context. We propose that, after the non-skeletogenic commitment, the EMM is divided into dermis and meninges by E11.5 in normal development. Two distinct responses of the EMM, chondrogenesis and osteogenesis, to Dlx5-augmentation in the NCC Dlx5 strongly support this idea.Tri H. VuMasaki TakechiMiki ShimizuTaro KitazawaHiroki HigashiyamaAkiyasu IwaseHiroki KuriharaSachiko IsekiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tri H. Vu Masaki Takechi Miki Shimizu Taro Kitazawa Hiroki Higashiyama Akiyasu Iwase Hiroki Kurihara Sachiko Iseki Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
description	Abstract Neural crest cells (NCCs) give rise to various tissues including neurons, pigment cells, bone and cartilage in the head. Distal-less homeobox 5 (Dlx5) is involved in both jaw patterning and differentiation of NCC-derivatives. In this study, we investigated the differentiation potential of head mesenchyme by forcing Dlx5 to be expressed in mouse NCC (NCC Dlx5 ). In NCC Dlx5 mice, differentiation of dermis and pigment cells were enhanced with ectopic cartilage (ec) and heterotopic bone (hb) in different layers at the cranial vertex. The ec and hb were derived from the early migrating mesenchyme (EMM), the non-skeletogenic cell population located above skeletogenic supraorbital mesenchyme (SOM). The ec developed within Foxc1 +-dura mater with increased PDGFRα signalling, and the hb formed with upregulation of BMP and WNT/β-catenin signallings in Dermo1 +-dermal layer from E11.5. Since dermal cells express Runx2 and Msx2 in the control, osteogenic potential in dermal cells seemed to be inhibited by an anti-osteogenic function of Msx2 in normal context. We propose that, after the non-skeletogenic commitment, the EMM is divided into dermis and meninges by E11.5 in normal development. Two distinct responses of the EMM, chondrogenesis and osteogenesis, to Dlx5-augmentation in the NCC Dlx5 strongly support this idea.
format	article
author	Tri H. Vu Masaki Takechi Miki Shimizu Taro Kitazawa Hiroki Higashiyama Akiyasu Iwase Hiroki Kurihara Sachiko Iseki
author_facet	Tri H. Vu Masaki Takechi Miki Shimizu Taro Kitazawa Hiroki Higashiyama Akiyasu Iwase Hiroki Kurihara Sachiko Iseki
author_sort	Tri H. Vu
title	Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
title_short	Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
title_full	Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
title_fullStr	Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
title_full_unstemmed	Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
title_sort	dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c786c597a874c7aa20414f54f069d87
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Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme

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