Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study

Oleogenic yeasts are characterized by the ability to accumulate increased amounts of lipids under certain conditions. These microbial lipids differ in their fatty acid composition, which allows them to be widely used in the biotechnology industry. The interest of biotechnologists is closely linked t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrea Němcová, Martin Szotkowski, Ota Samek, Linda Cagáňová, Matthias Sipiczki, Ivana Márová
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	yeasts <i>Metschnikowia</i> animal fat lipids stress factors Raman spectroscopy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4c7992153843400381cfec133b41e90d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4c7992153843400381cfec133b41e90d
record_format	dspace
spelling	oai:doaj.org-article:4c7992153843400381cfec133b41e90d2021-11-25T18:24:55ZUse of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study10.3390/microorganisms91122952076-2607https://doaj.org/article/4c7992153843400381cfec133b41e90d2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2295https://doaj.org/toc/2076-2607Oleogenic yeasts are characterized by the ability to accumulate increased amounts of lipids under certain conditions. These microbial lipids differ in their fatty acid composition, which allows them to be widely used in the biotechnology industry. The interest of biotechnologists is closely linked to the rising prices of fossil fuels in recent years. Their negative environmental impact is caused by significantly increased demand for biodiesel. The composition of microbial lipids is very similar to vegetable oils, which provides great potential for use in the production of biodiesel. In addition, some oleogenic microorganisms are capable of producing lipids with a high proportion of unsaturated fatty acids. The presented paper’s main aim was to study the production of lipids and lipid substances by yeasts of the genus <i>Metschnikowia</i>, to cultivate crude waste animal fat to study its utilization by yeasts, and to apply the idea of circular economy in the biotechnology of <i>Metschnikowia</i> yeasts. The work focuses on the influence of various stress factors in the cultivation process, such as reduced temperature or nutritional stress through the use of various waste substrates, together with manipulating the ratio of carbon and nitrogen sources in the medium. Yeast production properties were monitored by several instrumental techniques, including gas chromatography and Raman spectroscopy. The amount of lipids and in particular the fatty acid composition varied depending on the strains studied and the culture conditions used. The ability of yeast to produce significant amounts of unsaturated fatty acids was also demonstrated in the work. The most suitable substrate for lipid production was a medium containing glycerol, where the amount of accumulated lipids in the yeast <i>M. pulcherrima</i> 1232 was up to 36%. In our work, the crude animal fat was used for the production of high-value lipids, which to the best of our knowledge is a new result. Moreover, quantitative screening of lipase enzyme activity cultivated on animal fat substrate on selected yeasts of the genus <i>Metschnikowia</i> was performed. We found that for the yeast utilizing glycerol, animal fat seems to be an excellent source of carbon. Therefore, the yeast conversion of crude processed animal fat to value-added products is a valuable process for the biotechnology and food industry.Andrea NěmcováMartin SzotkowskiOta SamekLinda CagáňováMatthias SipiczkiIvana MárováMDPI AGarticleyeasts<i>Metschnikowia</i>animal fatlipidsstress factorsRaman spectroscopyBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2295, p 2295 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	yeasts <i>Metschnikowia</i> animal fat lipids stress factors Raman spectroscopy Biology (General) QH301-705.5
spellingShingle	yeasts <i>Metschnikowia</i> animal fat lipids stress factors Raman spectroscopy Biology (General) QH301-705.5 Andrea Němcová Martin Szotkowski Ota Samek Linda Cagáňová Matthias Sipiczki Ivana Márová Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study
description	Oleogenic yeasts are characterized by the ability to accumulate increased amounts of lipids under certain conditions. These microbial lipids differ in their fatty acid composition, which allows them to be widely used in the biotechnology industry. The interest of biotechnologists is closely linked to the rising prices of fossil fuels in recent years. Their negative environmental impact is caused by significantly increased demand for biodiesel. The composition of microbial lipids is very similar to vegetable oils, which provides great potential for use in the production of biodiesel. In addition, some oleogenic microorganisms are capable of producing lipids with a high proportion of unsaturated fatty acids. The presented paper’s main aim was to study the production of lipids and lipid substances by yeasts of the genus <i>Metschnikowia</i>, to cultivate crude waste animal fat to study its utilization by yeasts, and to apply the idea of circular economy in the biotechnology of <i>Metschnikowia</i> yeasts. The work focuses on the influence of various stress factors in the cultivation process, such as reduced temperature or nutritional stress through the use of various waste substrates, together with manipulating the ratio of carbon and nitrogen sources in the medium. Yeast production properties were monitored by several instrumental techniques, including gas chromatography and Raman spectroscopy. The amount of lipids and in particular the fatty acid composition varied depending on the strains studied and the culture conditions used. The ability of yeast to produce significant amounts of unsaturated fatty acids was also demonstrated in the work. The most suitable substrate for lipid production was a medium containing glycerol, where the amount of accumulated lipids in the yeast <i>M. pulcherrima</i> 1232 was up to 36%. In our work, the crude animal fat was used for the production of high-value lipids, which to the best of our knowledge is a new result. Moreover, quantitative screening of lipase enzyme activity cultivated on animal fat substrate on selected yeasts of the genus <i>Metschnikowia</i> was performed. We found that for the yeast utilizing glycerol, animal fat seems to be an excellent source of carbon. Therefore, the yeast conversion of crude processed animal fat to value-added products is a valuable process for the biotechnology and food industry.
format	article
author	Andrea Němcová Martin Szotkowski Ota Samek Linda Cagáňová Matthias Sipiczki Ivana Márová
author_facet	Andrea Němcová Martin Szotkowski Ota Samek Linda Cagáňová Matthias Sipiczki Ivana Márová
author_sort	Andrea Němcová
title	Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study
title_short	Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study
title_full	Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study
title_fullStr	Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study
title_full_unstemmed	Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study
title_sort	use of waste substrates for the lipid production by yeasts of the genus <em>metschnikowia</em>—screening study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c7992153843400381cfec133b41e90d
work_keys_str_mv	AT andreanemcova useofwastesubstratesforthelipidproductionbyyeastsofthegenusemmetschnikowiaemscreeningstudy AT martinszotkowski useofwastesubstratesforthelipidproductionbyyeastsofthegenusemmetschnikowiaemscreeningstudy AT otasamek useofwastesubstratesforthelipidproductionbyyeastsofthegenusemmetschnikowiaemscreeningstudy AT lindacaganova useofwastesubstratesforthelipidproductionbyyeastsofthegenusemmetschnikowiaemscreeningstudy AT matthiassipiczki useofwastesubstratesforthelipidproductionbyyeastsofthegenusemmetschnikowiaemscreeningstudy AT ivanamarova useofwastesubstratesforthelipidproductionbyyeastsofthegenusemmetschnikowiaemscreeningstudy
_version_	1718411192871944192

Use of Waste Substrates for the Lipid Production by Yeasts of the Genus <em>Metschnikowia</em>—Screening Study

Ejemplares similares