Tissue-specific expressed antibody variable gene repertoires.

Recent developments in genetic technologies allow deep analysis of the sequence diversity of immune repertoires, but little work has been reported on the architecture of immune repertoires in mucosal tissues. Antibodies are the key to prevention of infections at the mucosal surface, but it is curren...

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Autores principales: Bryan S Briney, Jordan R Willis, Jessica A Finn, Brett A McKinney, James E Crowe
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Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/4c8021a36a0c4f8e99150fe693d78d35
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spelling oai:doaj.org-article:4c8021a36a0c4f8e99150fe693d78d352021-11-11T08:21:57ZTissue-specific expressed antibody variable gene repertoires.1932-620310.1371/journal.pone.0100839https://doaj.org/article/4c8021a36a0c4f8e99150fe693d78d352014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24956460/?tool=EBIhttps://doaj.org/toc/1932-6203Recent developments in genetic technologies allow deep analysis of the sequence diversity of immune repertoires, but little work has been reported on the architecture of immune repertoires in mucosal tissues. Antibodies are the key to prevention of infections at the mucosal surface, but it is currently unclear whether the B cell repertoire at mucosal surfaces reflects the dominant antibodies found in the systemic compartment or whether mucosal tissues harbor unique repertoires. We examined the expressed antibody variable gene repertoires from 10 different human tissues using RNA samples derived from a large number of individuals. The results revealed that mucosal tissues such as stomach, intestine and lung possess unique antibody gene repertoires that differed substantially from those found in lymphoid tissues or peripheral blood. Mutation frequency analysis of mucosal tissue repertoires revealed that they were highly mutated, with little evidence for the presence of naïve B cells, in contrast to blood. Mucosal tissue repertoires possessed longer heavy chain complementarity determining region 3 loops than lymphoid tissue repertoires. We also noted a large increase in frequency of both insertions and deletions in the small intestine antibody repertoire. These data suggest that mucosal immune repertoires are distinct in many ways from the systemic compartment.Bryan S BrineyJordan R WillisJessica A FinnBrett A McKinneyJames E CrowePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100839 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Bryan S Briney
Jordan R Willis
Jessica A Finn
Brett A McKinney
James E Crowe
Tissue-specific expressed antibody variable gene repertoires.
description Recent developments in genetic technologies allow deep analysis of the sequence diversity of immune repertoires, but little work has been reported on the architecture of immune repertoires in mucosal tissues. Antibodies are the key to prevention of infections at the mucosal surface, but it is currently unclear whether the B cell repertoire at mucosal surfaces reflects the dominant antibodies found in the systemic compartment or whether mucosal tissues harbor unique repertoires. We examined the expressed antibody variable gene repertoires from 10 different human tissues using RNA samples derived from a large number of individuals. The results revealed that mucosal tissues such as stomach, intestine and lung possess unique antibody gene repertoires that differed substantially from those found in lymphoid tissues or peripheral blood. Mutation frequency analysis of mucosal tissue repertoires revealed that they were highly mutated, with little evidence for the presence of naïve B cells, in contrast to blood. Mucosal tissue repertoires possessed longer heavy chain complementarity determining region 3 loops than lymphoid tissue repertoires. We also noted a large increase in frequency of both insertions and deletions in the small intestine antibody repertoire. These data suggest that mucosal immune repertoires are distinct in many ways from the systemic compartment.
format article
author Bryan S Briney
Jordan R Willis
Jessica A Finn
Brett A McKinney
James E Crowe
author_facet Bryan S Briney
Jordan R Willis
Jessica A Finn
Brett A McKinney
James E Crowe
author_sort Bryan S Briney
title Tissue-specific expressed antibody variable gene repertoires.
title_short Tissue-specific expressed antibody variable gene repertoires.
title_full Tissue-specific expressed antibody variable gene repertoires.
title_fullStr Tissue-specific expressed antibody variable gene repertoires.
title_full_unstemmed Tissue-specific expressed antibody variable gene repertoires.
title_sort tissue-specific expressed antibody variable gene repertoires.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/4c8021a36a0c4f8e99150fe693d78d35
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AT jessicaafinn tissuespecificexpressedantibodyvariablegenerepertoires
AT brettamckinney tissuespecificexpressedantibodyvariablegenerepertoires
AT jamesecrowe tissuespecificexpressedantibodyvariablegenerepertoires
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