The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity

Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc sup...

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Autores principales: Tomoyuki Ueda, Masatoshi Inden, Katsuhiro Shirai, Shin-ichiro Sekine, Yuji Masaki, Hisaka Kurita, Kenji Ichihara, Takashi Inuzuka, Isao Hozumi
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/4c85d876879d47b186a4573347682a15
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spelling oai:doaj.org-article:4c85d876879d47b186a4573347682a152021-12-02T16:07:47ZThe effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity10.1038/s41598-017-03115-y2045-2322https://doaj.org/article/4c85d876879d47b186a4573347682a152017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03115-yhttps://doaj.org/toc/2045-2322Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc superoxide dismutase (SOD1)-mediated toxicity. Ethanol extract of brazilian green propolis (EBGP) protected N2a cells against mutant SOD1-induced neurotoxicity and reduced aggregated mutant SOD1 by induction of autophagy. Kaempferide and kaempferol, the active ingredients of EBGP, also inhibited mutant SOD1-induced cell death and reduced the intracellular mutant SOD1 aggregates. Both kaempferide and kaempferol significantly suppressed mutant SOD1-induced superoxide in mitochondria. Western blot analysis showed that kaempferol potentially induced autophagy via the AMP-activated protein kinase (AMPK) - the mammalian target of rapamycin (mTOR) pathway. These results suggest that EBGP containing the active ingredient against mutant SOD1-mediated toxicity is a promising medicine or health food for prevention and treatment of ALS.Tomoyuki UedaMasatoshi IndenKatsuhiro ShiraiShin-ichiro SekineYuji MasakiHisaka KuritaKenji IchiharaTakashi InuzukaIsao HozumiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Tomoyuki Ueda
Masatoshi Inden
Katsuhiro Shirai
Shin-ichiro Sekine
Yuji Masaki
Hisaka Kurita
Kenji Ichihara
Takashi Inuzuka
Isao Hozumi
The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
description Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc superoxide dismutase (SOD1)-mediated toxicity. Ethanol extract of brazilian green propolis (EBGP) protected N2a cells against mutant SOD1-induced neurotoxicity and reduced aggregated mutant SOD1 by induction of autophagy. Kaempferide and kaempferol, the active ingredients of EBGP, also inhibited mutant SOD1-induced cell death and reduced the intracellular mutant SOD1 aggregates. Both kaempferide and kaempferol significantly suppressed mutant SOD1-induced superoxide in mitochondria. Western blot analysis showed that kaempferol potentially induced autophagy via the AMP-activated protein kinase (AMPK) - the mammalian target of rapamycin (mTOR) pathway. These results suggest that EBGP containing the active ingredient against mutant SOD1-mediated toxicity is a promising medicine or health food for prevention and treatment of ALS.
format article
author Tomoyuki Ueda
Masatoshi Inden
Katsuhiro Shirai
Shin-ichiro Sekine
Yuji Masaki
Hisaka Kurita
Kenji Ichihara
Takashi Inuzuka
Isao Hozumi
author_facet Tomoyuki Ueda
Masatoshi Inden
Katsuhiro Shirai
Shin-ichiro Sekine
Yuji Masaki
Hisaka Kurita
Kenji Ichihara
Takashi Inuzuka
Isao Hozumi
author_sort Tomoyuki Ueda
title The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
title_short The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
title_full The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
title_fullStr The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
title_full_unstemmed The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
title_sort effects of brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/4c85d876879d47b186a4573347682a15
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