The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity
Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc sup...
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oai:doaj.org-article:4c85d876879d47b186a4573347682a152021-12-02T16:07:47ZThe effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity10.1038/s41598-017-03115-y2045-2322https://doaj.org/article/4c85d876879d47b186a4573347682a152017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03115-yhttps://doaj.org/toc/2045-2322Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc superoxide dismutase (SOD1)-mediated toxicity. Ethanol extract of brazilian green propolis (EBGP) protected N2a cells against mutant SOD1-induced neurotoxicity and reduced aggregated mutant SOD1 by induction of autophagy. Kaempferide and kaempferol, the active ingredients of EBGP, also inhibited mutant SOD1-induced cell death and reduced the intracellular mutant SOD1 aggregates. Both kaempferide and kaempferol significantly suppressed mutant SOD1-induced superoxide in mitochondria. Western blot analysis showed that kaempferol potentially induced autophagy via the AMP-activated protein kinase (AMPK) - the mammalian target of rapamycin (mTOR) pathway. These results suggest that EBGP containing the active ingredient against mutant SOD1-mediated toxicity is a promising medicine or health food for prevention and treatment of ALS.Tomoyuki UedaMasatoshi IndenKatsuhiro ShiraiShin-ichiro SekineYuji MasakiHisaka KuritaKenji IchiharaTakashi InuzukaIsao HozumiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Tomoyuki Ueda Masatoshi Inden Katsuhiro Shirai Shin-ichiro Sekine Yuji Masaki Hisaka Kurita Kenji Ichihara Takashi Inuzuka Isao Hozumi The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
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Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. The purpose of this study was to clarify effects of brazilian green propolis and the active ingredient against ALS-associated mutant copper-zinc superoxide dismutase (SOD1)-mediated toxicity. Ethanol extract of brazilian green propolis (EBGP) protected N2a cells against mutant SOD1-induced neurotoxicity and reduced aggregated mutant SOD1 by induction of autophagy. Kaempferide and kaempferol, the active ingredients of EBGP, also inhibited mutant SOD1-induced cell death and reduced the intracellular mutant SOD1 aggregates. Both kaempferide and kaempferol significantly suppressed mutant SOD1-induced superoxide in mitochondria. Western blot analysis showed that kaempferol potentially induced autophagy via the AMP-activated protein kinase (AMPK) - the mammalian target of rapamycin (mTOR) pathway. These results suggest that EBGP containing the active ingredient against mutant SOD1-mediated toxicity is a promising medicine or health food for prevention and treatment of ALS. |
format |
article |
author |
Tomoyuki Ueda Masatoshi Inden Katsuhiro Shirai Shin-ichiro Sekine Yuji Masaki Hisaka Kurita Kenji Ichihara Takashi Inuzuka Isao Hozumi |
author_facet |
Tomoyuki Ueda Masatoshi Inden Katsuhiro Shirai Shin-ichiro Sekine Yuji Masaki Hisaka Kurita Kenji Ichihara Takashi Inuzuka Isao Hozumi |
author_sort |
Tomoyuki Ueda |
title |
The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
title_short |
The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
title_full |
The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
title_fullStr |
The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
title_full_unstemmed |
The effects of Brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
title_sort |
effects of brazilian green propolis that contains flavonols against mutant copper-zinc superoxide dismutase-mediated toxicity |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/4c85d876879d47b186a4573347682a15 |
work_keys_str_mv |
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