SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids

ABSTRACT Amyloids are protein polymers that were initially linked to human diseases. Across the whole Tree of Life, many disease-unrelated proteins are now emerging for which amyloids represent distinct functional states. Most bacterial amyloids described are extracellular, contributing to biofilm f...

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Auteur principal:	Rafael Giraldo
Format:	article
Langue:	EN
Publié:	American Society for Microbiology 2020
Sujets:	RepA-WH1 bacterial amyloids prions protein engineering synthetic biology Microbiology QR1-502
Accès en ligne:	https://doaj.org/article/4c85ec2a2f854f4cb6c37b9a6dafce81
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id	oai:doaj.org-article:4c85ec2a2f854f4cb6c37b9a6dafce81
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spelling	oai:doaj.org-article:4c85ec2a2f854f4cb6c37b9a6dafce812021-12-02T18:44:38ZSynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids10.1128/mSystems.00553-202379-5077https://doaj.org/article/4c85ec2a2f854f4cb6c37b9a6dafce812020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00553-20https://doaj.org/toc/2379-5077ABSTRACT Amyloids are protein polymers that were initially linked to human diseases. Across the whole Tree of Life, many disease-unrelated proteins are now emerging for which amyloids represent distinct functional states. Most bacterial amyloids described are extracellular, contributing to biofilm formation. However, only a few have been found in the bacterial cytosol. This paper reviews from the perspective of synthetic biology (SynBio) our understanding of the subtle line that separates functional from pathogenic and transmissible amyloids (prions). In particular, it is focused on RepA-WH1, a functional albeit unconventional natural amyloidogenic protein domain that participates in controlling DNA replication of bacterial plasmids. SynBio approaches, including protein engineering and the design of allosteric effectors such as diverse ligands and an optogenetic module, have enabled the generation in RepA-WH1 of an intracellular cytotoxic prion-like agent in bacteria. The synthetic RepA-WH1 prion has the potential to develop into novel antimicrobials.Rafael GiraldoAmerican Society for MicrobiologyarticleRepA-WH1bacterial amyloidsprionsprotein engineeringsynthetic biologyMicrobiologyQR1-502ENmSystems, Vol 5, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	RepA-WH1 bacterial amyloids prions protein engineering synthetic biology Microbiology QR1-502
spellingShingle	RepA-WH1 bacterial amyloids prions protein engineering synthetic biology Microbiology QR1-502 Rafael Giraldo SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids
description	ABSTRACT Amyloids are protein polymers that were initially linked to human diseases. Across the whole Tree of Life, many disease-unrelated proteins are now emerging for which amyloids represent distinct functional states. Most bacterial amyloids described are extracellular, contributing to biofilm formation. However, only a few have been found in the bacterial cytosol. This paper reviews from the perspective of synthetic biology (SynBio) our understanding of the subtle line that separates functional from pathogenic and transmissible amyloids (prions). In particular, it is focused on RepA-WH1, a functional albeit unconventional natural amyloidogenic protein domain that participates in controlling DNA replication of bacterial plasmids. SynBio approaches, including protein engineering and the design of allosteric effectors such as diverse ligands and an optogenetic module, have enabled the generation in RepA-WH1 of an intracellular cytotoxic prion-like agent in bacteria. The synthetic RepA-WH1 prion has the potential to develop into novel antimicrobials.
format	article
author	Rafael Giraldo
author_facet	Rafael Giraldo
author_sort	Rafael Giraldo
title	SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids
title_short	SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids
title_full	SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids
title_fullStr	SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids
title_full_unstemmed	SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids
title_sort	synbio and the boundaries between functional and pathogenic repa-wh1 bacterial amyloids
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/4c85ec2a2f854f4cb6c37b9a6dafce81
work_keys_str_mv	AT rafaelgiraldo synbioandtheboundariesbetweenfunctionalandpathogenicrepawh1bacterialamyloids
_version_	1718377679045001216

SynBio and the Boundaries between Functional and Pathogenic RepA-WH1 Bacterial Amyloids

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