Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair

Abstract The repair of skeletal defects in maxillofacial region remains an intractable problem, the rising technology of bone tissue engineering provides a new strategy to solve it. Scaffolds, a crucial element of tissue engineering, must have favorable biocompatibility as well as osteoinductivity....

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Autores principales:	Lan Ma, Yijun Yu, Hanxiao Liu, Weibin Sun, Zitong Lin, Chao Liu, Leiying Miao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c8adb90e3c049ce9a53666924597410
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spelling	oai:doaj.org-article:4c8adb90e3c049ce9a536669245974102021-12-02T14:12:40ZBerberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair10.1038/s41598-020-79734-92045-2322https://doaj.org/article/4c8adb90e3c049ce9a536669245974102021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79734-9https://doaj.org/toc/2045-2322Abstract The repair of skeletal defects in maxillofacial region remains an intractable problem, the rising technology of bone tissue engineering provides a new strategy to solve it. Scaffolds, a crucial element of tissue engineering, must have favorable biocompatibility as well as osteoinductivity. In this study, we prepared berberine/polycaprolactone/collagen (BBR/PCL/COL) scaffolds with different concentrations of berberine (BBR) (25, 50, 75 and 100 μg/mL) through electrospinning. The influence of dosage on scaffold morphology, cell behavior and in vivo bone defect repair were systematically studied. The results indicated that scaffolds could release BBR stably for up to 27 days. Experiments in vitro showed that BBR/PCL/COL scaffolds had appropriate biocompatibility in the concentration of 25–75 μg/mL, and 50 and 75 μg/mL scaffolds could significantly promote osteogenic differentiation of dental pulp stem cells. Scaffold with 50 μg/mL BBR was implanted into the critical bone defect of rats to evaluate the ability of bone repair in vivo. It was found that BBR/PCL/COL scaffold performed more favorable than polycaprolactone/collagen (PCL/COL) scaffold. Overall, our study is the first to evaluate the capability of in vivo bone repair of BBR/PCL/COL electrospun scaffold. The results indicate that BBR/PCL/COL scaffold has prospective potential for tissue engineering applications in bone regeneration therapy.Lan MaYijun YuHanxiao LiuWeibin SunZitong LinChao LiuLeiying MiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lan Ma Yijun Yu Hanxiao Liu Weibin Sun Zitong Lin Chao Liu Leiying Miao Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair
description	Abstract The repair of skeletal defects in maxillofacial region remains an intractable problem, the rising technology of bone tissue engineering provides a new strategy to solve it. Scaffolds, a crucial element of tissue engineering, must have favorable biocompatibility as well as osteoinductivity. In this study, we prepared berberine/polycaprolactone/collagen (BBR/PCL/COL) scaffolds with different concentrations of berberine (BBR) (25, 50, 75 and 100 μg/mL) through electrospinning. The influence of dosage on scaffold morphology, cell behavior and in vivo bone defect repair were systematically studied. The results indicated that scaffolds could release BBR stably for up to 27 days. Experiments in vitro showed that BBR/PCL/COL scaffolds had appropriate biocompatibility in the concentration of 25–75 μg/mL, and 50 and 75 μg/mL scaffolds could significantly promote osteogenic differentiation of dental pulp stem cells. Scaffold with 50 μg/mL BBR was implanted into the critical bone defect of rats to evaluate the ability of bone repair in vivo. It was found that BBR/PCL/COL scaffold performed more favorable than polycaprolactone/collagen (PCL/COL) scaffold. Overall, our study is the first to evaluate the capability of in vivo bone repair of BBR/PCL/COL electrospun scaffold. The results indicate that BBR/PCL/COL scaffold has prospective potential for tissue engineering applications in bone regeneration therapy.
format	article
author	Lan Ma Yijun Yu Hanxiao Liu Weibin Sun Zitong Lin Chao Liu Leiying Miao
author_facet	Lan Ma Yijun Yu Hanxiao Liu Weibin Sun Zitong Lin Chao Liu Leiying Miao
author_sort	Lan Ma
title	Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair
title_short	Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair
title_full	Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair
title_fullStr	Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair
title_full_unstemmed	Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair
title_sort	berberine-releasing electrospun scaffold induces osteogenic differentiation of dpscs and accelerates bone repair
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c8adb90e3c049ce9a53666924597410
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Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair

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