Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment

Abstract In quantitative PCR research, appropriate reference genes are key to determining accurate mRNA expression levels. In order to screen the reference genes suitable for detecting gene expression in tissues of the reproductive axis, a total of 420 (males and females = 1:5) 3-year-old Magang gee...

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Autores principales:	Bei-Bei Zhang, Xu Shen, Xiu-Jin Li, Yun-Bo Tian, Hong-Jia Ouyang, Yun-Mao Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c8fec74b95247b6837cbeedd6f5bdf3
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spelling	oai:doaj.org-article:4c8fec74b95247b6837cbeedd6f5bdf32021-12-02T14:37:21ZReference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment10.1038/s41598-021-87169-z2045-2322https://doaj.org/article/4c8fec74b95247b6837cbeedd6f5bdf32021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87169-zhttps://doaj.org/toc/2045-2322Abstract In quantitative PCR research, appropriate reference genes are key to determining accurate mRNA expression levels. In order to screen the reference genes suitable for detecting gene expression in tissues of the reproductive axis, a total of 420 (males and females = 1:5) 3-year-old Magang geese were selected and subjected to light treatment. The hypothalamus, pituitary and testicular tissues were subsequently collected at different stages. Ten genes including HPRT1, GAPDH, ACTB, LDHA, SDHA, B2M, TUBB4, TFRC, RPS2 and RPL4 were selected as candidate reference genes. The expression of these genes in goose reproductive axis tissues was detected by real-time fluorescent quantitative PCR. The ΔCT, geNorm, NormFinder and BestKeeper algorithms were applied to sort gene expression according to stability. The results showed that ACTB and TUBB4 were the most suitable reference genes for the hypothalamic tissue of Magang goose in the three breeding stages; HPRT1 and RPL4 for pituitary tissue; and HPRT1 and LDHA for testicular tissue. For all three reproductive axis tissues, ACTB was the most suitable reference gene, whereas the least stable reference gene was GAPDH. Altogether, these results can provide references for tissue expression studies in geese under light treatment.Bei-Bei ZhangXu ShenXiu-Jin LiYun-Bo TianHong-Jia OuyangYun-Mao HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bei-Bei Zhang Xu Shen Xiu-Jin Li Yun-Bo Tian Hong-Jia Ouyang Yun-Mao Huang Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment
description	Abstract In quantitative PCR research, appropriate reference genes are key to determining accurate mRNA expression levels. In order to screen the reference genes suitable for detecting gene expression in tissues of the reproductive axis, a total of 420 (males and females = 1:5) 3-year-old Magang geese were selected and subjected to light treatment. The hypothalamus, pituitary and testicular tissues were subsequently collected at different stages. Ten genes including HPRT1, GAPDH, ACTB, LDHA, SDHA, B2M, TUBB4, TFRC, RPS2 and RPL4 were selected as candidate reference genes. The expression of these genes in goose reproductive axis tissues was detected by real-time fluorescent quantitative PCR. The ΔCT, geNorm, NormFinder and BestKeeper algorithms were applied to sort gene expression according to stability. The results showed that ACTB and TUBB4 were the most suitable reference genes for the hypothalamic tissue of Magang goose in the three breeding stages; HPRT1 and RPL4 for pituitary tissue; and HPRT1 and LDHA for testicular tissue. For all three reproductive axis tissues, ACTB was the most suitable reference gene, whereas the least stable reference gene was GAPDH. Altogether, these results can provide references for tissue expression studies in geese under light treatment.
format	article
author	Bei-Bei Zhang Xu Shen Xiu-Jin Li Yun-Bo Tian Hong-Jia Ouyang Yun-Mao Huang
author_facet	Bei-Bei Zhang Xu Shen Xiu-Jin Li Yun-Bo Tian Hong-Jia Ouyang Yun-Mao Huang
author_sort	Bei-Bei Zhang
title	Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment
title_short	Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment
title_full	Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment
title_fullStr	Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment
title_full_unstemmed	Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment
title_sort	reference gene selection for expression studies in the reproductive axis tissues of magang geese at different reproductive stages under light treatment
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c8fec74b95247b6837cbeedd6f5bdf3
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Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment

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