Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype

Abstract Shiga toxin (Stx) is the key virulent factor in Shiga toxin-producing Escherichia coli (STEC). To date, three Stx1 subtypes and seven Stx2 subtypes have been described in E. coli, which differed in receptor preference and toxin potency. Here, we identified a novel Stx2 subtype designated St...

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Autores principales:	Xiangning Bai, Shanshan Fu, Ji Zhang, Ruyue Fan, Yanmei Xu, Hui Sun, Xiaohua He, Jianguo Xu, Yanwen Xiong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c90919fb83844cb8d8b22c445aab51d
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spelling	oai:doaj.org-article:4c90919fb83844cb8d8b22c445aab51d2021-12-02T15:07:59ZIdentification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype10.1038/s41598-018-25233-x2045-2322https://doaj.org/article/4c90919fb83844cb8d8b22c445aab51d2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25233-xhttps://doaj.org/toc/2045-2322Abstract Shiga toxin (Stx) is the key virulent factor in Shiga toxin-producing Escherichia coli (STEC). To date, three Stx1 subtypes and seven Stx2 subtypes have been described in E. coli, which differed in receptor preference and toxin potency. Here, we identified a novel Stx2 subtype designated Stx2h in E. coli strains isolated from wild marmots in the Qinghai-Tibetan plateau, China. Stx2h shares 91.9% nucleic acid sequence identity and 92.9% amino acid identity to the nearest Stx2 subtype. The expression of Stx2h in type strain STEC299 was inducible by mitomycin C, and culture supernatant from STEC299 was cytotoxic to Vero cells. The Stx2h converting prophage was unique in terms of insertion site and genetic composition. Whole genome-based phylo- and patho-genomic analysis revealed STEC299 was closer to other pathotypes of E. coli than STEC, and possesses virulence factors from other pathotypes. Our finding enlarges the pool of Stx2 subtypes and highlights the extraordinary genomic plasticity of E. coli strains. As the emergence of new Shiga toxin genotypes and new Stx-producing pathotypes pose a great threat to the public health, Stx2h should be further included in E. coli molecular typing, and in epidemiological surveillance of E. coli infections.Xiangning BaiShanshan FuJi ZhangRuyue FanYanmei XuHui SunXiaohua HeJianguo XuYanwen XiongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangning Bai Shanshan Fu Ji Zhang Ruyue Fan Yanmei Xu Hui Sun Xiaohua He Jianguo Xu Yanwen Xiong Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
description	Abstract Shiga toxin (Stx) is the key virulent factor in Shiga toxin-producing Escherichia coli (STEC). To date, three Stx1 subtypes and seven Stx2 subtypes have been described in E. coli, which differed in receptor preference and toxin potency. Here, we identified a novel Stx2 subtype designated Stx2h in E. coli strains isolated from wild marmots in the Qinghai-Tibetan plateau, China. Stx2h shares 91.9% nucleic acid sequence identity and 92.9% amino acid identity to the nearest Stx2 subtype. The expression of Stx2h in type strain STEC299 was inducible by mitomycin C, and culture supernatant from STEC299 was cytotoxic to Vero cells. The Stx2h converting prophage was unique in terms of insertion site and genetic composition. Whole genome-based phylo- and patho-genomic analysis revealed STEC299 was closer to other pathotypes of E. coli than STEC, and possesses virulence factors from other pathotypes. Our finding enlarges the pool of Stx2 subtypes and highlights the extraordinary genomic plasticity of E. coli strains. As the emergence of new Shiga toxin genotypes and new Stx-producing pathotypes pose a great threat to the public health, Stx2h should be further included in E. coli molecular typing, and in epidemiological surveillance of E. coli infections.
format	article
author	Xiangning Bai Shanshan Fu Ji Zhang Ruyue Fan Yanmei Xu Hui Sun Xiaohua He Jianguo Xu Yanwen Xiong
author_facet	Xiangning Bai Shanshan Fu Ji Zhang Ruyue Fan Yanmei Xu Hui Sun Xiaohua He Jianguo Xu Yanwen Xiong
author_sort	Xiangning Bai
title	Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
title_short	Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
title_full	Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
title_fullStr	Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
title_full_unstemmed	Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
title_sort	identification and pathogenomic analysis of an escherichia coli strain producing a novel shiga toxin 2 subtype
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4c90919fb83844cb8d8b22c445aab51d
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_version_	1718388313442746368

Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype

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