Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies

Colon cancer-associated gut bacteria were mostly identified via next-generation sequencing in gut microbiome profiling studies. Anaerobic culture systems can be used to culture colon cells with these gut bacteria to further confirm the tumorigenic properties of these bacteria. Nevertheless, it is un...

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Autores principales:	Muhammad Nur `Adam Hatta, Ezanee Azlina Mohamad Hanif, Siok-Fong Chin, Hui-min Neoh
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2021
Materias:	anaerobic culture system cell viability colorectal cancer ht-29 hypoxic markers Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4c927dcee4ae4efa852054220a41c60d
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spelling	oai:doaj.org-article:4c927dcee4ae4efa852054220a41c60d2021-11-05T16:47:43ZPrerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies2503-217810.14710/jbtr.v7i1.10129https://doaj.org/article/4c927dcee4ae4efa852054220a41c60d2021-04-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/10129https://doaj.org/toc/2503-2178Colon cancer-associated gut bacteria were mostly identified via next-generation sequencing in gut microbiome profiling studies. Anaerobic culture systems can be used to culture colon cells with these gut bacteria to further confirm the tumorigenic properties of these bacteria. Nevertheless, it is unclear how colon cells will grow in an anaerobic environment, as most cells are cultured aerobically. Therefore, we investigated the survival and viability of HT-29, a colon cancer cell line in an anaerobic culture system, and compared it to the usual culture condition in an aerobic setup. Interestingly, we found that HT-29 was able to grow in the anaerobic setup. Its viability was similar for both culture conditions, with only a slower growth rate observed in the anaerobic setup. Furthermore, gene expression studies showed that the cells were not under severe anaerobic stress even when exposed to the oxygen-deprived environment.This study provided results on some baseline parameters of an anaerobic colon cell culture system, and will be useful for journal readers who wish to investigate functional properties of anaerobic bacteria.Muhammad Nur `Adam HattaEzanee Azlina Mohamad HanifSiok-Fong ChinHui-min NeohDiponegoro Universityarticleanaerobic culture systemcell viabilitycolorectal cancerht-29hypoxic markersMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 7, Iss 1, Pp 48-50 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anaerobic culture system cell viability colorectal cancer ht-29 hypoxic markers Medicine (General) R5-920
spellingShingle	anaerobic culture system cell viability colorectal cancer ht-29 hypoxic markers Medicine (General) R5-920 Muhammad Nur `Adam Hatta Ezanee Azlina Mohamad Hanif Siok-Fong Chin Hui-min Neoh Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies
description	Colon cancer-associated gut bacteria were mostly identified via next-generation sequencing in gut microbiome profiling studies. Anaerobic culture systems can be used to culture colon cells with these gut bacteria to further confirm the tumorigenic properties of these bacteria. Nevertheless, it is unclear how colon cells will grow in an anaerobic environment, as most cells are cultured aerobically. Therefore, we investigated the survival and viability of HT-29, a colon cancer cell line in an anaerobic culture system, and compared it to the usual culture condition in an aerobic setup. Interestingly, we found that HT-29 was able to grow in the anaerobic setup. Its viability was similar for both culture conditions, with only a slower growth rate observed in the anaerobic setup. Furthermore, gene expression studies showed that the cells were not under severe anaerobic stress even when exposed to the oxygen-deprived environment.This study provided results on some baseline parameters of an anaerobic colon cell culture system, and will be useful for journal readers who wish to investigate functional properties of anaerobic bacteria.
format	article
author	Muhammad Nur `Adam Hatta Ezanee Azlina Mohamad Hanif Siok-Fong Chin Hui-min Neoh
author_facet	Muhammad Nur `Adam Hatta Ezanee Azlina Mohamad Hanif Siok-Fong Chin Hui-min Neoh
author_sort	Muhammad Nur `Adam Hatta
title	Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies
title_short	Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies
title_full	Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies
title_fullStr	Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies
title_full_unstemmed	Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies
title_sort	prerequisite evaluation of anaerobic settings for gut microbiome functional studies
publisher	Diponegoro University
publishDate	2021
url	https://doaj.org/article/4c927dcee4ae4efa852054220a41c60d
work_keys_str_mv	AT muhammadnuradamhatta prerequisiteevaluationofanaerobicsettingsforgutmicrobiomefunctionalstudies AT ezaneeazlinamohamadhanif prerequisiteevaluationofanaerobicsettingsforgutmicrobiomefunctionalstudies AT siokfongchin prerequisiteevaluationofanaerobicsettingsforgutmicrobiomefunctionalstudies AT huiminneoh prerequisiteevaluationofanaerobicsettingsforgutmicrobiomefunctionalstudies
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Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies

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