A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.

<h4>Background</h4>The FtsK DNA-translocase controls the last steps of chromosome segregation in E. coli. It translocates sister chromosomes using the KOPS DNA motifs to orient its activity, and controls the resolution of dimeric forms of sister chromosomes by XerCD-mediated recombinatio...

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Autores principales: Marie Deghorain, Carine Pagès, Jean-Christophe Meile, Mathieu Stouf, Hervé Capiaux, Romain Mercier, Christian Lesterlin, Bernard Hallet, François Cornet
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:4c98f06dea824713aa780b79d34886e82021-11-18T06:49:53ZA defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.1932-620310.1371/journal.pone.0022164https://doaj.org/article/4c98f06dea824713aa780b79d34886e82011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21799784/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The FtsK DNA-translocase controls the last steps of chromosome segregation in E. coli. It translocates sister chromosomes using the KOPS DNA motifs to orient its activity, and controls the resolution of dimeric forms of sister chromosomes by XerCD-mediated recombination at the dif site and their decatenation by TopoIV.<h4>Methodology</h4>We have used XerCD/dif recombination as a genetic trap to probe the interaction of FtsK with loci located in different regions of the chromosome. This assay revealed that the activity of FtsK is restricted to a ∼400 kb terminal region of the chromosome around the natural position of the dif site. Preferential interaction with this region required the tethering of FtsK to the division septum via its N-terminal domain as well as its translocation activity. However, the KOPS-recognition activity of FtsK was not required. Displacement of replication termination outside the FtsK high activity region had no effect on FtsK activity and deletion of a part of this region was not compensated by its extension to neighbouring regions. By observing the fate of fluorescent-tagged loci of the ter region, we found that segregation of the FtsK high activity region is delayed compared to that of its adjacent regions.<h4>Significance</h4>Our results show that a restricted terminal region of the chromosome is specifically dedicated to the last steps of chromosome segregation and to their coupling with cell division by FtsK.Marie DeghorainCarine PagèsJean-Christophe MeileMathieu StoufHervé CapiauxRomain MercierChristian LesterlinBernard HalletFrançois CornetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22164 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Marie Deghorain
Carine Pagès
Jean-Christophe Meile
Mathieu Stouf
Hervé Capiaux
Romain Mercier
Christian Lesterlin
Bernard Hallet
François Cornet
A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
description <h4>Background</h4>The FtsK DNA-translocase controls the last steps of chromosome segregation in E. coli. It translocates sister chromosomes using the KOPS DNA motifs to orient its activity, and controls the resolution of dimeric forms of sister chromosomes by XerCD-mediated recombination at the dif site and their decatenation by TopoIV.<h4>Methodology</h4>We have used XerCD/dif recombination as a genetic trap to probe the interaction of FtsK with loci located in different regions of the chromosome. This assay revealed that the activity of FtsK is restricted to a ∼400 kb terminal region of the chromosome around the natural position of the dif site. Preferential interaction with this region required the tethering of FtsK to the division septum via its N-terminal domain as well as its translocation activity. However, the KOPS-recognition activity of FtsK was not required. Displacement of replication termination outside the FtsK high activity region had no effect on FtsK activity and deletion of a part of this region was not compensated by its extension to neighbouring regions. By observing the fate of fluorescent-tagged loci of the ter region, we found that segregation of the FtsK high activity region is delayed compared to that of its adjacent regions.<h4>Significance</h4>Our results show that a restricted terminal region of the chromosome is specifically dedicated to the last steps of chromosome segregation and to their coupling with cell division by FtsK.
format article
author Marie Deghorain
Carine Pagès
Jean-Christophe Meile
Mathieu Stouf
Hervé Capiaux
Romain Mercier
Christian Lesterlin
Bernard Hallet
François Cornet
author_facet Marie Deghorain
Carine Pagès
Jean-Christophe Meile
Mathieu Stouf
Hervé Capiaux
Romain Mercier
Christian Lesterlin
Bernard Hallet
François Cornet
author_sort Marie Deghorain
title A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
title_short A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
title_full A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
title_fullStr A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
title_full_unstemmed A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
title_sort defined terminal region of the e. coli chromosome shows late segregation and high ftsk activity.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/4c98f06dea824713aa780b79d34886e8
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