Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds

Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluat...

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Autores principales:	Oikonomidis Ioannis L., Kiossis Evangelos, Brozos Christos, Kritsepi-Konstantinou Maria
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2019
Materias:	biochemistry endocrinology hematology reference interval sheep variation Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4c99801c8583432f86b61fa51a38f5f0
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spelling	oai:doaj.org-article:4c99801c8583432f86b61fa51a38f5f02021-11-17T21:27:53ZEffect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds1820-744810.2478/acve-2019-0037https://doaj.org/article/4c99801c8583432f86b61fa51a38f5f02019-12-01T00:00:00Zhttps://doi.org/10.2478/acve-2019-0037https://doaj.org/toc/1820-7448Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluate the effect of season on hematologic and biochemical analytes, and serum total thyroxine and cortisol in adult rams of two breeds. Four blood samples (one every season) were collected from each ram. Complete blood count was performed on the Advia 120 (Siemens Healthcare Diagnostics, USA), while the differential leukocyte count was manually conducted. Biochemical and hormonal analyses were performed on Flexor E (Vital Scientific, The Netherlands), AVL 9180 (Roche Diagnostics, Belgium), and Immulite 1000 (Siemens Healthcare Diagnostics, USA), respectively. Linear mixed effects models (R language) were employed for statistical analyses. Forty-three (26 Chios, 17 Florina), adult, clinically healthy rams were included. Statistically significant (p<0.05), mostly breed-independent seasonal differences were observed in almost all of the analytes. However, when assessing these differences in view of the respective reference intervals, only a few of them were considered biologically important. Specifically, mild hyperglycemia and mild decrease in the concentration of total calcium and inorganic phosphorus were detected in winter, while a mild increase in thyroxine concentration (autumn) and creatine kinase activity (spring and summer) was also noted. In conclusion, seasonal effects should be considered when evaluating laboratory results in rams; however, season does not appear to have an essential effect on the clinicopathological profile of rams reared in the Mediterranean region.Oikonomidis Ioannis L.Kiossis EvangelosBrozos ChristosKritsepi-Konstantinou MariaSciendoarticlebiochemistryendocrinologyhematologyreference intervalsheepvariationVeterinary medicineSF600-1100ENActa Veterinaria, Vol 69, Iss 4, Pp 434-442 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	biochemistry endocrinology hematology reference interval sheep variation Veterinary medicine SF600-1100
spellingShingle	biochemistry endocrinology hematology reference interval sheep variation Veterinary medicine SF600-1100 Oikonomidis Ioannis L. Kiossis Evangelos Brozos Christos Kritsepi-Konstantinou Maria Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
description	Clinicopathological investigations are essential for the evaluation of the health status of ruminants. Apart from species-specific reference intervals, the effect of common biological factors should be considered for an accurate interpretation of laboratory data. The aim of this study was to evaluate the effect of season on hematologic and biochemical analytes, and serum total thyroxine and cortisol in adult rams of two breeds. Four blood samples (one every season) were collected from each ram. Complete blood count was performed on the Advia 120 (Siemens Healthcare Diagnostics, USA), while the differential leukocyte count was manually conducted. Biochemical and hormonal analyses were performed on Flexor E (Vital Scientific, The Netherlands), AVL 9180 (Roche Diagnostics, Belgium), and Immulite 1000 (Siemens Healthcare Diagnostics, USA), respectively. Linear mixed effects models (R language) were employed for statistical analyses. Forty-three (26 Chios, 17 Florina), adult, clinically healthy rams were included. Statistically significant (p<0.05), mostly breed-independent seasonal differences were observed in almost all of the analytes. However, when assessing these differences in view of the respective reference intervals, only a few of them were considered biologically important. Specifically, mild hyperglycemia and mild decrease in the concentration of total calcium and inorganic phosphorus were detected in winter, while a mild increase in thyroxine concentration (autumn) and creatine kinase activity (spring and summer) was also noted. In conclusion, seasonal effects should be considered when evaluating laboratory results in rams; however, season does not appear to have an essential effect on the clinicopathological profile of rams reared in the Mediterranean region.
format	article
author	Oikonomidis Ioannis L. Kiossis Evangelos Brozos Christos Kritsepi-Konstantinou Maria
author_facet	Oikonomidis Ioannis L. Kiossis Evangelos Brozos Christos Kritsepi-Konstantinou Maria
author_sort	Oikonomidis Ioannis L.
title	Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
title_short	Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
title_full	Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
title_fullStr	Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
title_full_unstemmed	Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds
title_sort	effect of season on hematologic, biochemical, and hormonal analytes in rams of two breeds
publisher	Sciendo
publishDate	2019
url	https://doaj.org/article/4c99801c8583432f86b61fa51a38f5f0
work_keys_str_mv	AT oikonomidisioannisl effectofseasononhematologicbiochemicalandhormonalanalytesinramsoftwobreeds AT kiossisevangelos effectofseasononhematologicbiochemicalandhormonalanalytesinramsoftwobreeds AT brozoschristos effectofseasononhematologicbiochemicalandhormonalanalytesinramsoftwobreeds AT kritsepikonstantinoumaria effectofseasononhematologicbiochemicalandhormonalanalytesinramsoftwobreeds
_version_	1718425280341606400

Effect of Season on Hematologic, Biochemical, and Hormonal Analytes in Rams of Two Breeds

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