Mutations in the C-terminus of HBoV NS1 affect the function of NP1

Abstract Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP...

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Autores principales:	Junmei Zhang, Yan Bai, Bing Zhu, Sujuan Hao, Zhen Chen, Hanzhong Wang, Wuxiang Guan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c9ae7e602524ea99bc6368ba0148dc2
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spelling	oai:doaj.org-article:4c9ae7e602524ea99bc6368ba0148dc22021-12-02T12:31:49ZMutations in the C-terminus of HBoV NS1 affect the function of NP110.1038/s41598-017-06513-42045-2322https://doaj.org/article/4c9ae7e602524ea99bc6368ba0148dc22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06513-4https://doaj.org/toc/2045-2322Abstract Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP1 expression in the recombinant infectious clone, on which the C-terminus of NS1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. The result suggested that NP1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where NP1 is essential for viral replication. Further studies showed that clinical mutations in the NP1 region did not affect viral genome replication, and UP1 promoted viral DNA replication. Our results suggested that the C-terminus of NS1 is important for viral replication and may be a target for regulating the replication of the viral genome.Junmei ZhangYan BaiBing ZhuSujuan HaoZhen ChenHanzhong WangWuxiang GuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Junmei Zhang Yan Bai Bing Zhu Sujuan Hao Zhen Chen Hanzhong Wang Wuxiang Guan Mutations in the C-terminus of HBoV NS1 affect the function of NP1
description	Abstract Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP1 expression in the recombinant infectious clone, on which the C-terminus of NS1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. The result suggested that NP1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where NP1 is essential for viral replication. Further studies showed that clinical mutations in the NP1 region did not affect viral genome replication, and UP1 promoted viral DNA replication. Our results suggested that the C-terminus of NS1 is important for viral replication and may be a target for regulating the replication of the viral genome.
format	article
author	Junmei Zhang Yan Bai Bing Zhu Sujuan Hao Zhen Chen Hanzhong Wang Wuxiang Guan
author_facet	Junmei Zhang Yan Bai Bing Zhu Sujuan Hao Zhen Chen Hanzhong Wang Wuxiang Guan
author_sort	Junmei Zhang
title	Mutations in the C-terminus of HBoV NS1 affect the function of NP1
title_short	Mutations in the C-terminus of HBoV NS1 affect the function of NP1
title_full	Mutations in the C-terminus of HBoV NS1 affect the function of NP1
title_fullStr	Mutations in the C-terminus of HBoV NS1 affect the function of NP1
title_full_unstemmed	Mutations in the C-terminus of HBoV NS1 affect the function of NP1
title_sort	mutations in the c-terminus of hbov ns1 affect the function of np1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4c9ae7e602524ea99bc6368ba0148dc2
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Mutations in the C-terminus of HBoV NS1 affect the function of NP1

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