Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

Abstract Bioremediation offers a viable alternative for the reduction of contaminants from the environment, particularly petroleum and its recalcitrant derivatives. In this study, the ability of a strain of Pseudomonas BUN14 to degrade crude oil, pristane and dioxin compounds, and to produce biosurf...

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Autores principales:	Mouna Mahjoubi, Habibu Aliyu, Mohamed Neifar, Simone Cappello, Habib Chouchane, Yasmine Souissi, Ahmed Salaheddine Masmoudi, Don A. Cowan, Ameur Cherif
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c9c416b874247138a94f7fe423745d8
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spelling	oai:doaj.org-article:4c9c416b874247138a94f7fe423745d82021-12-02T18:03:07ZGenomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN1410.1038/s41598-021-87487-22045-2322https://doaj.org/article/4c9c416b874247138a94f7fe423745d82021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87487-2https://doaj.org/toc/2045-2322Abstract Bioremediation offers a viable alternative for the reduction of contaminants from the environment, particularly petroleum and its recalcitrant derivatives. In this study, the ability of a strain of Pseudomonas BUN14 to degrade crude oil, pristane and dioxin compounds, and to produce biosurfactants, was investigated. BUN14 is a halotolerant strain isolated from polluted sediment recovered from the refinery harbor on the Bizerte coast, north Tunisia and capable of producing surfactants. The strain BUN14 was assembled into 22 contigs of 4,898,053 bp with a mean GC content of 62.4%. Whole genome phylogeny and comparative genome analyses showed that strain BUN14 could be affiliated with two validly described Pseudomonas Type Strains, P. kunmingensis DSM 25974T and P. chloritidismutans AW-1T. The current study, however, revealed that the two Type Strains are probably conspecific and, given the priority of the latter, we proposed that P. kunmingensis DSM 25974 is a heteronym of P. chloritidismutans AW-1T. Using GC-FID analysis, we determined that BUN14 was able to use a range of hydrocarbons (crude oil, pristane, dibenzofuran, dibenzothiophene, naphthalene) as a sole carbon source. Genome analysis of BUN14 revealed the presence of a large repertoire of proteins (154) related to xenobiotic biodegradation and metabolism. Thus, 44 proteins were linked to the pathways for complete degradation of benzoate and naphthalene. The annotation of conserved functional domains led to the detection of putative genes encoding enzymes of the rhamnolipid biosynthesis pathway. Overall, the polyvalent hydrocarbon degradation capacity of BUN14 makes it a promising candidate for application in the bioremediation of polluted saline environments.Mouna MahjoubiHabibu AliyuMohamed NeifarSimone CappelloHabib ChouchaneYasmine SouissiAhmed Salaheddine MasmoudiDon A. CowanAmeur CherifNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mouna Mahjoubi Habibu Aliyu Mohamed Neifar Simone Cappello Habib Chouchane Yasmine Souissi Ahmed Salaheddine Masmoudi Don A. Cowan Ameur Cherif Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
description	Abstract Bioremediation offers a viable alternative for the reduction of contaminants from the environment, particularly petroleum and its recalcitrant derivatives. In this study, the ability of a strain of Pseudomonas BUN14 to degrade crude oil, pristane and dioxin compounds, and to produce biosurfactants, was investigated. BUN14 is a halotolerant strain isolated from polluted sediment recovered from the refinery harbor on the Bizerte coast, north Tunisia and capable of producing surfactants. The strain BUN14 was assembled into 22 contigs of 4,898,053 bp with a mean GC content of 62.4%. Whole genome phylogeny and comparative genome analyses showed that strain BUN14 could be affiliated with two validly described Pseudomonas Type Strains, P. kunmingensis DSM 25974T and P. chloritidismutans AW-1T. The current study, however, revealed that the two Type Strains are probably conspecific and, given the priority of the latter, we proposed that P. kunmingensis DSM 25974 is a heteronym of P. chloritidismutans AW-1T. Using GC-FID analysis, we determined that BUN14 was able to use a range of hydrocarbons (crude oil, pristane, dibenzofuran, dibenzothiophene, naphthalene) as a sole carbon source. Genome analysis of BUN14 revealed the presence of a large repertoire of proteins (154) related to xenobiotic biodegradation and metabolism. Thus, 44 proteins were linked to the pathways for complete degradation of benzoate and naphthalene. The annotation of conserved functional domains led to the detection of putative genes encoding enzymes of the rhamnolipid biosynthesis pathway. Overall, the polyvalent hydrocarbon degradation capacity of BUN14 makes it a promising candidate for application in the bioremediation of polluted saline environments.
format	article
author	Mouna Mahjoubi Habibu Aliyu Mohamed Neifar Simone Cappello Habib Chouchane Yasmine Souissi Ahmed Salaheddine Masmoudi Don A. Cowan Ameur Cherif
author_facet	Mouna Mahjoubi Habibu Aliyu Mohamed Neifar Simone Cappello Habib Chouchane Yasmine Souissi Ahmed Salaheddine Masmoudi Don A. Cowan Ameur Cherif
author_sort	Mouna Mahjoubi
title	Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
title_short	Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
title_full	Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
title_fullStr	Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
title_full_unstemmed	Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
title_sort	genomic characterization of a polyvalent hydrocarbonoclastic bacterium pseudomonas sp. strain bun14
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c9c416b874247138a94f7fe423745d8
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Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

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