Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators

Abstract This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using ‘U’ shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded i...

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Autores principales:	Swathi Chaudhari, Kamalesh Chaudhari, Seokbeom Kim, Faheem Khan, Jungchul Lee, Thomas Thundat
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ca52c634fe446e0bc446f7bc824ea19
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spelling	oai:doaj.org-article:4ca52c634fe446e0bc446f7bc824ea192021-12-02T16:08:20ZElectrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators10.1038/s41598-017-03846-y2045-2322https://doaj.org/article/4ca52c634fe446e0bc446f7bc824ea192017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03846-yhttps://doaj.org/toc/2045-2322Abstract This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using ‘U’ shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded in the HNR preventing direct in-situ electrophoretic separation. The flow of analytes through the HNR was achieved by balancing the hydrodynamic pressure to override the electromotive force and inhibit the motion of analytes towards the anode for capillary electrophoresis. The resonance frequency shifts of the HNR vibrating around 1.5 MHz were correlated with the time of the passage of the protein bands to construct the mass spectrum. The proposed concept was demonstrated by constructing a mass spectrum of egg white proteins in the molecular weight range of 14–250 kDa. When compared to regular polyacrylamide gel electrophoresis, our method not only provides a precise and fast readout but also avoids the use of chemical staining. This study paves a new route for low-cost and on-chip mass spectrometers with ultra-miniaturized dimensions.Swathi ChaudhariKamalesh ChaudhariSeokbeom KimFaheem KhanJungchul LeeThomas ThundatNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Swathi Chaudhari Kamalesh Chaudhari Seokbeom Kim Faheem Khan Jungchul Lee Thomas Thundat Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
description	Abstract This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using ‘U’ shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded in the HNR preventing direct in-situ electrophoretic separation. The flow of analytes through the HNR was achieved by balancing the hydrodynamic pressure to override the electromotive force and inhibit the motion of analytes towards the anode for capillary electrophoresis. The resonance frequency shifts of the HNR vibrating around 1.5 MHz were correlated with the time of the passage of the protein bands to construct the mass spectrum. The proposed concept was demonstrated by constructing a mass spectrum of egg white proteins in the molecular weight range of 14–250 kDa. When compared to regular polyacrylamide gel electrophoresis, our method not only provides a precise and fast readout but also avoids the use of chemical staining. This study paves a new route for low-cost and on-chip mass spectrometers with ultra-miniaturized dimensions.
format	article
author	Swathi Chaudhari Kamalesh Chaudhari Seokbeom Kim Faheem Khan Jungchul Lee Thomas Thundat
author_facet	Swathi Chaudhari Kamalesh Chaudhari Seokbeom Kim Faheem Khan Jungchul Lee Thomas Thundat
author_sort	Swathi Chaudhari
title	Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
title_short	Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
title_full	Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
title_fullStr	Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
title_full_unstemmed	Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
title_sort	electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4ca52c634fe446e0bc446f7bc824ea19
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Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators

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