Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma

Abstract Objectives To investigate the regulation of the N‐6‐methyladenosine (m6A) methyltransferases METTL3, METTL14, WTAP, KIAA1429, and METTL4, referred to as “m6A writers,” in clear cell renal cell carcinoma (ccRCC), and other RCC subtypes in respect of the potential prognostic value. Patients a...

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Autores principales:	Larissa Gundert, Alexander Strick, Felix vonHagen, Doris Schmidt, Niklas Klümper, Yuri Tolkach, Marieta Toma, Glen Kristiansen, Manuel Ritter, Jörg Ellinger
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	ccRCC KIAA1429 m6A METTL3 METTL14 METTL4 Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/4ca73d20926b4acb80b4b8068223207f
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spelling	oai:doaj.org-article:4ca73d20926b4acb80b4b8068223207f2021-11-17T16:19:36ZSystematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma2688-452610.1002/bco2.89https://doaj.org/article/4ca73d20926b4acb80b4b8068223207f2021-11-01T00:00:00Zhttps://doi.org/10.1002/bco2.89https://doaj.org/toc/2688-4526Abstract Objectives To investigate the regulation of the N‐6‐methyladenosine (m6A) methyltransferases METTL3, METTL14, WTAP, KIAA1429, and METTL4, referred to as “m6A writers,” in clear cell renal cell carcinoma (ccRCC), and other RCC subtypes in respect of the potential prognostic value. Patients and methods Tissue samples were collected within the framework of the Biobank at the Center for Integrated Oncology Bonn. The expression of the methyltransferases was systematically determined in clear cell renal carcinoma (ccRCC) on the RNA (real‐time PCR) and protein level (immunohistochemistry). Additionally, protein expression of the m6A writers was further investigated in papillary RCC, chromophobe RCC, sarcomatoid RCC, oncocytoma, and normal renal tissue (immunohistochemistry). Results The expression of all m6A‐methyltransferases was significantly downregulated in ccRCC compared to benign renal tissue. Low m6A‐methyltransferase levels were correlated with higher histological grade, advanced pT‐stage, pN‐stage, and metastatic disease. Reduced m6A‐methyltransferase expression was associated with shorter overall survival. Conclusion In conclusion, m6A‐methyltransferases are dysregulated in ccRCC and might act as tumor suppressor genes, which could be of particular importance for future diagnostic and therapeutic options.Larissa GundertAlexander StrickFelix vonHagenDoris SchmidtNiklas KlümperYuri TolkachMarieta TomaGlen KristiansenManuel RitterJörg EllingerWileyarticleccRCCKIAA1429m6AMETTL3METTL14METTL4Diseases of the genitourinary system. UrologyRC870-923ENBJUI Compass, Vol 2, Iss 6, Pp 402-411 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ccRCC KIAA1429 m6A METTL3 METTL14 METTL4 Diseases of the genitourinary system. Urology RC870-923
spellingShingle	ccRCC KIAA1429 m6A METTL3 METTL14 METTL4 Diseases of the genitourinary system. Urology RC870-923 Larissa Gundert Alexander Strick Felix vonHagen Doris Schmidt Niklas Klümper Yuri Tolkach Marieta Toma Glen Kristiansen Manuel Ritter Jörg Ellinger Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma
description	Abstract Objectives To investigate the regulation of the N‐6‐methyladenosine (m6A) methyltransferases METTL3, METTL14, WTAP, KIAA1429, and METTL4, referred to as “m6A writers,” in clear cell renal cell carcinoma (ccRCC), and other RCC subtypes in respect of the potential prognostic value. Patients and methods Tissue samples were collected within the framework of the Biobank at the Center for Integrated Oncology Bonn. The expression of the methyltransferases was systematically determined in clear cell renal carcinoma (ccRCC) on the RNA (real‐time PCR) and protein level (immunohistochemistry). Additionally, protein expression of the m6A writers was further investigated in papillary RCC, chromophobe RCC, sarcomatoid RCC, oncocytoma, and normal renal tissue (immunohistochemistry). Results The expression of all m6A‐methyltransferases was significantly downregulated in ccRCC compared to benign renal tissue. Low m6A‐methyltransferase levels were correlated with higher histological grade, advanced pT‐stage, pN‐stage, and metastatic disease. Reduced m6A‐methyltransferase expression was associated with shorter overall survival. Conclusion In conclusion, m6A‐methyltransferases are dysregulated in ccRCC and might act as tumor suppressor genes, which could be of particular importance for future diagnostic and therapeutic options.
format	article
author	Larissa Gundert Alexander Strick Felix vonHagen Doris Schmidt Niklas Klümper Yuri Tolkach Marieta Toma Glen Kristiansen Manuel Ritter Jörg Ellinger
author_facet	Larissa Gundert Alexander Strick Felix vonHagen Doris Schmidt Niklas Klümper Yuri Tolkach Marieta Toma Glen Kristiansen Manuel Ritter Jörg Ellinger
author_sort	Larissa Gundert
title	Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma
title_short	Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma
title_full	Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma
title_fullStr	Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma
title_full_unstemmed	Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma
title_sort	systematic expression analysis of m6a rna methyltransferases in clear cell renal cell carcinoma
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/4ca73d20926b4acb80b4b8068223207f
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_version_	1718425414162972672

Systematic expression analysis of m6A RNA methyltransferases in clear cell renal cell carcinoma

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