Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.

<h4>Background</h4>Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis. The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial communities of Bt-maize has been monitored over a per...

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Autores principales:	Jorge Barriuso, José R Valverde, Rafael P Mellado
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ca8736eec0448e2a92b97641aabc3cd
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spelling	oai:doaj.org-article:4ca8736eec0448e2a92b97641aabc3cd2021-11-18T07:20:13ZEffect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.1932-620310.1371/journal.pone.0035481https://doaj.org/article/4ca8736eec0448e2a92b97641aabc3cd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558158/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis. The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial communities of Bt-maize has been monitored over a period of four years.<h4>Methodology/principal findings</h4>The accumulative effects of the cultivation of this transgenic plant have been monitored by means of high throughput DNA pyrosequencing of the bacterial DNA coding for the 16S rRNA hypervariable V6 region from rhizobacterial communities. The obtained sequences were subjected to taxonomic, phylogenetic and taxonomic-independent diversity studies. The results obtained were consistent, indicating that variations detected in the rhizobacterial community structure were possibly due to climatic factors rather than to the presence of the Bt-gene. No variations were observed in the diversity estimates between non-Bt and Bt-maize.<h4>Conclusions/significance</h4>The cultivation of Bt-maize during the four-year period did not change the maize rhizobacterial communities when compared to those of the non-Bt maize. This is the first study to be conducted with Bt-maize during such a long cultivation period and the first evaluation of rhizobacterial communities to be performed in this transgenic plant using Next Generation Sequencing.Jorge BarriusoJosé R ValverdeRafael P MelladoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35481 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jorge Barriuso José R Valverde Rafael P Mellado Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.
description	<h4>Background</h4>Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis. The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial communities of Bt-maize has been monitored over a period of four years.<h4>Methodology/principal findings</h4>The accumulative effects of the cultivation of this transgenic plant have been monitored by means of high throughput DNA pyrosequencing of the bacterial DNA coding for the 16S rRNA hypervariable V6 region from rhizobacterial communities. The obtained sequences were subjected to taxonomic, phylogenetic and taxonomic-independent diversity studies. The results obtained were consistent, indicating that variations detected in the rhizobacterial community structure were possibly due to climatic factors rather than to the presence of the Bt-gene. No variations were observed in the diversity estimates between non-Bt and Bt-maize.<h4>Conclusions/significance</h4>The cultivation of Bt-maize during the four-year period did not change the maize rhizobacterial communities when compared to those of the non-Bt maize. This is the first study to be conducted with Bt-maize during such a long cultivation period and the first evaluation of rhizobacterial communities to be performed in this transgenic plant using Next Generation Sequencing.
format	article
author	Jorge Barriuso José R Valverde Rafael P Mellado
author_facet	Jorge Barriuso José R Valverde Rafael P Mellado
author_sort	Jorge Barriuso
title	Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.
title_short	Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.
title_full	Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.
title_fullStr	Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.
title_full_unstemmed	Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.
title_sort	effect of cry1ab protein on rhizobacterial communities of bt-maize over a four-year cultivation period.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/4ca8736eec0448e2a92b97641aabc3cd
work_keys_str_mv	AT jorgebarriuso effectofcry1abproteinonrhizobacterialcommunitiesofbtmaizeoverafouryearcultivationperiod AT joservalverde effectofcry1abproteinonrhizobacterialcommunitiesofbtmaizeoverafouryearcultivationperiod AT rafaelpmellado effectofcry1abproteinonrhizobacterialcommunitiesofbtmaizeoverafouryearcultivationperiod
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Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period.

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