Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation

Abstract Introduction Graft‐versus‐host disease (GVHD) is frequent and fatal complication following allogeneic hematopoietic stem cell transplantation (HSCT) and characteristically involves skin, gut, and liver. Macrophages promote tissue regeneration and mediate immunomodulation. Macrophages are di...

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Autores principales:	Ryo Hanaki, Hidemi Toyoda, Shotaro Iwamoto, Mari Morimoto, Daisuke Nakato, Takahiro Ito, Kaori Niwa, Keishiro Amano, Ryotaro Hashizume, Isao Tawara, Masahiro Hirayama
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	graft‐versus‐host disease (GVHD) hematopoietic stem cell transplantation (HSCT) M1 macrophage M2 macrophage M‐CSF Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4ca97194d62e4f2aae0ccf11a866334a
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spelling	oai:doaj.org-article:4ca97194d62e4f2aae0ccf11a866334a2021-11-12T19:57:15ZDonor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation2050-452710.1002/iid3.503https://doaj.org/article/4ca97194d62e4f2aae0ccf11a866334a2021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.503https://doaj.org/toc/2050-4527Abstract Introduction Graft‐versus‐host disease (GVHD) is frequent and fatal complication following allogeneic hematopoietic stem cell transplantation (HSCT) and characteristically involves skin, gut, and liver. Macrophages promote tissue regeneration and mediate immunomodulation. Macrophages are divided into two different phenotypes, classically activated M1 (pro‐inflammatory or immune‐reactive macrophages) and alternatively activated M2 (anti‐inflammatory or immune‐suppressive macrophages). The anti‐inflammatory effect of M2 macrophage led us to test its effect in the pathophysiology of GVHD. Methods GVHD was induced in lethally irradiated BALB/c mice. M2 macrophages derived from donor bone marrow (BM) were administered intravenously, while controls received donor BM‐mononuclear cells and splenocytes. Animals were monitored for clinical GVHD and analyzed. Results We confirmed that administering donor BM‐derived M2 macrophages attenuated GVHD severity and prolonged survival after HSCT. Moreover, donor BM‐derived M2 macrophages significantly suppressed donor T cell proliferation by cell‐to‐cell contact in vitro. Conclusions We showed the protective effects of donor‐derived M2 macrophages on GVHD and improved survival in a model of HSCT. Our data suggest that donor‐derived M2 macrophages offer the potential for cell‐based therapy to treat GVHD.Ryo HanakiHidemi ToyodaShotaro IwamotoMari MorimotoDaisuke NakatoTakahiro ItoKaori NiwaKeishiro AmanoRyotaro HashizumeIsao TawaraMasahiro HirayamaWileyarticlegraft‐versus‐host disease (GVHD)hematopoietic stem cell transplantation (HSCT)M1 macrophageM2 macrophageM‐CSFImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1489-1499 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	graft‐versus‐host disease (GVHD) hematopoietic stem cell transplantation (HSCT) M1 macrophage M2 macrophage M‐CSF Immunologic diseases. Allergy RC581-607
spellingShingle	graft‐versus‐host disease (GVHD) hematopoietic stem cell transplantation (HSCT) M1 macrophage M2 macrophage M‐CSF Immunologic diseases. Allergy RC581-607 Ryo Hanaki Hidemi Toyoda Shotaro Iwamoto Mari Morimoto Daisuke Nakato Takahiro Ito Kaori Niwa Keishiro Amano Ryotaro Hashizume Isao Tawara Masahiro Hirayama Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation
description	Abstract Introduction Graft‐versus‐host disease (GVHD) is frequent and fatal complication following allogeneic hematopoietic stem cell transplantation (HSCT) and characteristically involves skin, gut, and liver. Macrophages promote tissue regeneration and mediate immunomodulation. Macrophages are divided into two different phenotypes, classically activated M1 (pro‐inflammatory or immune‐reactive macrophages) and alternatively activated M2 (anti‐inflammatory or immune‐suppressive macrophages). The anti‐inflammatory effect of M2 macrophage led us to test its effect in the pathophysiology of GVHD. Methods GVHD was induced in lethally irradiated BALB/c mice. M2 macrophages derived from donor bone marrow (BM) were administered intravenously, while controls received donor BM‐mononuclear cells and splenocytes. Animals were monitored for clinical GVHD and analyzed. Results We confirmed that administering donor BM‐derived M2 macrophages attenuated GVHD severity and prolonged survival after HSCT. Moreover, donor BM‐derived M2 macrophages significantly suppressed donor T cell proliferation by cell‐to‐cell contact in vitro. Conclusions We showed the protective effects of donor‐derived M2 macrophages on GVHD and improved survival in a model of HSCT. Our data suggest that donor‐derived M2 macrophages offer the potential for cell‐based therapy to treat GVHD.
format	article
author	Ryo Hanaki Hidemi Toyoda Shotaro Iwamoto Mari Morimoto Daisuke Nakato Takahiro Ito Kaori Niwa Keishiro Amano Ryotaro Hashizume Isao Tawara Masahiro Hirayama
author_facet	Ryo Hanaki Hidemi Toyoda Shotaro Iwamoto Mari Morimoto Daisuke Nakato Takahiro Ito Kaori Niwa Keishiro Amano Ryotaro Hashizume Isao Tawara Masahiro Hirayama
author_sort	Ryo Hanaki
title	Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation
title_short	Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation
title_full	Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation
title_fullStr	Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation
title_full_unstemmed	Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation
title_sort	donor‐derived m2 macrophages attenuate gvhd after allogeneic hematopoietic stem cell transplantation
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/4ca97194d62e4f2aae0ccf11a866334a
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Donor‐derived M2 macrophages attenuate GVHD after allogeneic hematopoietic stem cell transplantation

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