PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.

Three-dimensional (3D) in vitro cell based assays for Prostate Cancer (PCa) research are rapidly becoming the preferred alternative to that of conventional 2D monolayer cultures. 3D assays more precisely mimic the microenvironment found in vivo, and thus are ideally suited to evaluate compounds and...

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Autores principales:	Md Tamjidul Hoque, Louisa C E Windus, Carrie J Lovitt, Vicky M Avery
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ca97e96d6954dc9bded45e57de67f35
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spelling	oai:doaj.org-article:4ca97e96d6954dc9bded45e57de67f352021-11-18T08:45:31ZPCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.1932-620310.1371/journal.pone.0079865https://doaj.org/article/4ca97e96d6954dc9bded45e57de67f352013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278197/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Three-dimensional (3D) in vitro cell based assays for Prostate Cancer (PCa) research are rapidly becoming the preferred alternative to that of conventional 2D monolayer cultures. 3D assays more precisely mimic the microenvironment found in vivo, and thus are ideally suited to evaluate compounds and their suitability for progression in the drug discovery pipeline. To achieve the desired high throughput needed for most screening programs, automated quantification of 3D cultures is required. Towards this end, this paper reports on the development of a prototype analysis module for an automated high-content-analysis (HCA) system, which allows for accurate and fast investigation of in vitro 3D cell culture models for PCa. The Java based program, which we have named PCaAnalyser, uses novel algorithms that allow accurate and rapid quantitation of protein expression in 3D cell culture. As currently configured, the PCaAnalyser can quantify a range of biological parameters including: nuclei-count, nuclei-spheroid membership prediction, various function based classification of peripheral and non-peripheral areas to measure expression of biomarkers and protein constituents known to be associated with PCa progression, as well as defining segregate cellular-objects effectively for a range of signal-to-noise ratios. In addition, PCaAnalyser architecture is highly flexible, operating as a single independent analysis, as well as in batch mode; essential for High-Throughput-Screening (HTS). Utilising the PCaAnalyser, accurate and rapid analysis in an automated high throughput manner is provided, and reproducible analysis of the distribution and intensity of well-established markers associated with PCa progression in a range of metastatic PCa cell-lines (DU145 and PC3) in a 3D model demonstrated.Md Tamjidul HoqueLouisa C E WindusCarrie J LovittVicky M AveryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79865 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Md Tamjidul Hoque Louisa C E Windus Carrie J Lovitt Vicky M Avery PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
description	Three-dimensional (3D) in vitro cell based assays for Prostate Cancer (PCa) research are rapidly becoming the preferred alternative to that of conventional 2D monolayer cultures. 3D assays more precisely mimic the microenvironment found in vivo, and thus are ideally suited to evaluate compounds and their suitability for progression in the drug discovery pipeline. To achieve the desired high throughput needed for most screening programs, automated quantification of 3D cultures is required. Towards this end, this paper reports on the development of a prototype analysis module for an automated high-content-analysis (HCA) system, which allows for accurate and fast investigation of in vitro 3D cell culture models for PCa. The Java based program, which we have named PCaAnalyser, uses novel algorithms that allow accurate and rapid quantitation of protein expression in 3D cell culture. As currently configured, the PCaAnalyser can quantify a range of biological parameters including: nuclei-count, nuclei-spheroid membership prediction, various function based classification of peripheral and non-peripheral areas to measure expression of biomarkers and protein constituents known to be associated with PCa progression, as well as defining segregate cellular-objects effectively for a range of signal-to-noise ratios. In addition, PCaAnalyser architecture is highly flexible, operating as a single independent analysis, as well as in batch mode; essential for High-Throughput-Screening (HTS). Utilising the PCaAnalyser, accurate and rapid analysis in an automated high throughput manner is provided, and reproducible analysis of the distribution and intensity of well-established markers associated with PCa progression in a range of metastatic PCa cell-lines (DU145 and PC3) in a 3D model demonstrated.
format	article
author	Md Tamjidul Hoque Louisa C E Windus Carrie J Lovitt Vicky M Avery
author_facet	Md Tamjidul Hoque Louisa C E Windus Carrie J Lovitt Vicky M Avery
author_sort	Md Tamjidul Hoque
title	PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
title_short	PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
title_full	PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
title_fullStr	PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
title_full_unstemmed	PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
title_sort	pcaanalyser: a 2d-image analysis based module for effective determination of prostate cancer progression in 3d culture.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4ca97e96d6954dc9bded45e57de67f35
work_keys_str_mv	AT mdtamjidulhoque pcaanalysera2dimageanalysisbasedmoduleforeffectivedeterminationofprostatecancerprogressionin3dculture AT louisacewindus pcaanalysera2dimageanalysisbasedmoduleforeffectivedeterminationofprostatecancerprogressionin3dculture AT carriejlovitt pcaanalysera2dimageanalysisbasedmoduleforeffectivedeterminationofprostatecancerprogressionin3dculture AT vickymavery pcaanalysera2dimageanalysisbasedmoduleforeffectivedeterminationofprostatecancerprogressionin3dculture
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PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.

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