ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS

Tick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belon...

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Autores principales:	I. S. Solovarov, M. A. Khasnatinov, G. A. Danchinova, A. V. Liapunov, N. A. Bolotova, E. L. Manzarova, I. G. Kondratov, S. I. Belikov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	aptamers tick-borne encephalitis virus plaque-forming units Science Q
Acceso en línea:	https://doaj.org/article/4cb60a9be9694de18acc44eb946be947
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spelling	oai:doaj.org-article:4cb60a9be9694de18acc44eb946be9472021-11-23T06:14:37ZASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS2541-94202587-959610.12737/article_5955e6b5aad2e3.30269730https://doaj.org/article/4cb60a9be9694de18acc44eb946be9472017-02-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/369https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Tick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belonging to the family Flaviviridae, genus Flavivirus. In spite the significant impact of TBE on human health, there is a serious lack of specific treatment against this disease. The only specific drug available is the human anti-TBEV immunoglobulin from vaccinated blood donors. The drug is produced and used in Russia only, both to prevent and to cure the TBE. In this work, we evaluated the ability of TBEV-specific DNA-aptamers and extracts of traditional medicine plants to neutralize the TBEV Selection of aptamers was performed using SELEX approach. Extracts of the seeds of Momordica cochinchinensis and Terminalia chebula were produced by boiling the ground seeds in water, clarified by centrifugation and filtration steps and filter sterilized. The SELEX had produced two aptamers - My13 and My38. Neither of two was capable to neutralize TBEV in vitro. The ability to shield the antibody binding sites on the surface of TBEV virions was also absent. The extract of M. cochinchinensis exhibited no neutralizing activity as well. Surprisingly, the T. chebula extract completely neutralized the TBEV after 30 min of incubation at 37 °C. The possible explanations and further development of the project are discussed.I. S. SolovarovM. A. KhasnatinovG. A. DanchinovaA. V. LiapunovN. A. BolotovaE. L. ManzarovaI. G. KondratovS. I. BelikovScientific Сentre for Family Health and Human Reproduction Problemsarticleaptamerstick-borne encephalitis virusplaque-forming unitsScienceQRUActa Biomedica Scientifica, Vol 2, Iss 1, Pp 84-88 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	aptamers tick-borne encephalitis virus plaque-forming units Science Q
spellingShingle	aptamers tick-borne encephalitis virus plaque-forming units Science Q I. S. Solovarov M. A. Khasnatinov G. A. Danchinova A. V. Liapunov N. A. Bolotova E. L. Manzarova I. G. Kondratov S. I. Belikov ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
description	Tick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belonging to the family Flaviviridae, genus Flavivirus. In spite the significant impact of TBE on human health, there is a serious lack of specific treatment against this disease. The only specific drug available is the human anti-TBEV immunoglobulin from vaccinated blood donors. The drug is produced and used in Russia only, both to prevent and to cure the TBE. In this work, we evaluated the ability of TBEV-specific DNA-aptamers and extracts of traditional medicine plants to neutralize the TBEV Selection of aptamers was performed using SELEX approach. Extracts of the seeds of Momordica cochinchinensis and Terminalia chebula were produced by boiling the ground seeds in water, clarified by centrifugation and filtration steps and filter sterilized. The SELEX had produced two aptamers - My13 and My38. Neither of two was capable to neutralize TBEV in vitro. The ability to shield the antibody binding sites on the surface of TBEV virions was also absent. The extract of M. cochinchinensis exhibited no neutralizing activity as well. Surprisingly, the T. chebula extract completely neutralized the TBEV after 30 min of incubation at 37 °C. The possible explanations and further development of the project are discussed.
format	article
author	I. S. Solovarov M. A. Khasnatinov G. A. Danchinova A. V. Liapunov N. A. Bolotova E. L. Manzarova I. G. Kondratov S. I. Belikov
author_facet	I. S. Solovarov M. A. Khasnatinov G. A. Danchinova A. V. Liapunov N. A. Bolotova E. L. Manzarova I. G. Kondratov S. I. Belikov
author_sort	I. S. Solovarov
title	ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
title_short	ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
title_full	ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
title_fullStr	ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
title_full_unstemmed	ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS
title_sort	assessment of neutralizing properties of dna-aptamers and extracts of medicinal herbs against the tick-borne encephalitis virus
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/4cb60a9be9694de18acc44eb946be947
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ASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUS

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