Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei

Ficus hirta Vahl. has been reported to have hepatoprotective, antitumor, antibacterial functions, and is used to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Ammonia nitrogen is one of the most common environmental stress factors in aquaculture. Long-term exposure to high conc...

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Autores principales:	QingJian Liang, WenNa Dong, FeiFei Wang, WeiNa Wang, Jian Zhang, Xing Liu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Penaeus vannamei miR-2765 Antioxidant Ficus hirta Vahl Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/4cb9570759204f0e9eeb72cb2a021c40
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spelling	oai:doaj.org-article:4cb9570759204f0e9eeb72cb2a021c402021-11-18T04:43:17ZFicus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei0147-651310.1016/j.ecoenv.2021.112989https://doaj.org/article/4cb9570759204f0e9eeb72cb2a021c402021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011015https://doaj.org/toc/0147-6513Ficus hirta Vahl. has been reported to have hepatoprotective, antitumor, antibacterial functions, and is used to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Ammonia nitrogen is one of the most common environmental stress factors in aquaculture. Long-term exposure to high concentrations of ammonia nitrogen can induce oxidative stress and increase the risk of infections. However, whether Ficus hirta Vahl. has effect on ammonia nitrogen stress is unclear. In present study we report that Ficus hirta Vahl. improves the activity of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) of shrimp and decreases shrimp mortality caused by ammonia nitrogen stress. It is demonstrated that miR-2765 is negatively regulate the antioxidant capacity. We find that SOD was a direct target gene of miR-2765. MiR-2765 can bind to 3′-untranslated region (3′-UTR) of SOD to inhibit its transcription. Furthermore, Ficus hirta Vahl. down-regulates miR-2765 to activate the antioxidant capacity to alleviate the damage caused by ammonia nitrogen stress. Interestingly, overexpression of miR-2765 could attenuate the protective effect of Ficus hirta Vahl. on shrimp under ammonia nitrogen stress. These data indicate that Ficus hirta Vahl. alleviates the damage of ammonia nitrogen stress in shrimp by repressing miR-2765 and activating the antioxidant enzyme system. This study will provide a theoretical basis and a new perspective for assessing the toxicity mechanism of ammonia nitrogen in the process of farming on shrimp.QingJian LiangWenNa DongFeiFei WangWeiNa WangJian ZhangXing LiuElsevierarticlePenaeus vannameimiR-2765AntioxidantFicus hirta VahlEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112989- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Penaeus vannamei miR-2765 Antioxidant Ficus hirta Vahl Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Penaeus vannamei miR-2765 Antioxidant Ficus hirta Vahl Environmental pollution TD172-193.5 Environmental sciences GE1-350 QingJian Liang WenNa Dong FeiFei Wang WeiNa Wang Jian Zhang Xing Liu Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei
description	Ficus hirta Vahl. has been reported to have hepatoprotective, antitumor, antibacterial functions, and is used to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Ammonia nitrogen is one of the most common environmental stress factors in aquaculture. Long-term exposure to high concentrations of ammonia nitrogen can induce oxidative stress and increase the risk of infections. However, whether Ficus hirta Vahl. has effect on ammonia nitrogen stress is unclear. In present study we report that Ficus hirta Vahl. improves the activity of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) of shrimp and decreases shrimp mortality caused by ammonia nitrogen stress. It is demonstrated that miR-2765 is negatively regulate the antioxidant capacity. We find that SOD was a direct target gene of miR-2765. MiR-2765 can bind to 3′-untranslated region (3′-UTR) of SOD to inhibit its transcription. Furthermore, Ficus hirta Vahl. down-regulates miR-2765 to activate the antioxidant capacity to alleviate the damage caused by ammonia nitrogen stress. Interestingly, overexpression of miR-2765 could attenuate the protective effect of Ficus hirta Vahl. on shrimp under ammonia nitrogen stress. These data indicate that Ficus hirta Vahl. alleviates the damage of ammonia nitrogen stress in shrimp by repressing miR-2765 and activating the antioxidant enzyme system. This study will provide a theoretical basis and a new perspective for assessing the toxicity mechanism of ammonia nitrogen in the process of farming on shrimp.
format	article
author	QingJian Liang WenNa Dong FeiFei Wang WeiNa Wang Jian Zhang Xing Liu
author_facet	QingJian Liang WenNa Dong FeiFei Wang WeiNa Wang Jian Zhang Xing Liu
author_sort	QingJian Liang
title	Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei
title_short	Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei
title_full	Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei
title_fullStr	Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei
title_full_unstemmed	Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei
title_sort	ficus hirta vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting mir-2765 expression in penaeus vannamei
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4cb9570759204f0e9eeb72cb2a021c40
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Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei

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