Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia

Nurhatika S, Ermavitalini D, Saputro TB, Apriyatmoko Y. 2018. Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia. Biodiversitas 19: 627-632. Microalgae are aquatic unicellular or multicellular microscopic photoautotroph organisms. High lip...

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Autores principales: SRI NURHATIKA, DINI ERMAVITALINI, TRIONO BAGUS SAPUTRO, YUDI APRIYATMOKO
Formato: article
Lenguaje:EN
Publicado: MBI & UNS Solo 2018
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Acceso en línea:https://doaj.org/article/4ccbcc683d404c178fa79e65ab2f5ed3
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spelling oai:doaj.org-article:4ccbcc683d404c178fa79e65ab2f5ed32021-11-16T14:01:41ZBiodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia1412-033X2085-472210.13057/biodiv/d190234https://doaj.org/article/4ccbcc683d404c178fa79e65ab2f5ed32018-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2546https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Nurhatika S, Ermavitalini D, Saputro TB, Apriyatmoko Y. 2018. Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia. Biodiversitas 19: 627-632. Microalgae are aquatic unicellular or multicellular microscopic photoautotroph organisms. High lipid content in microalgae biomass can be used as raw material for biodiesel. Porong river estuary is one of the sites of industrial waste disposal. The polluted waters considered will affect the lipid microalgae metabolism. The main research aims are to isolate and characterize of high lipid content microalgae found in Porong river estuary through the selection of qualitative lipid content. Sampling was conducted at three different stations. Isolation was conducted in solid agar media enriched with Porong River Estuary sterile water and using streak plate method. The isolated microalgae were characterized using a light microscope and to be matched with identification books. The lipid content was determined qualitatively by coloring the isolated-microalgae cells with Nile Red, an intracellular lipid dye then observed its luminescence color under a fluorescence microscope. Several genera of microalgae that can be isolated in this study are Oscillatoria, Nitzschia, Merismopedia, Navicula, Nannochloropsis, and Melosira. The results of qualitative lipid analysis show that the genus Nannochloropsis and Nitzschia have high intracellular lipid accumulation.SRI NURHATIKADINI ERMAVITALINITRIONO BAGUS SAPUTROYUDI APRIYATMOKOMBI & UNS Soloarticlebiodieselnannochloropsisnitzschiaporong river estuaryqualitative lipid contentBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 2, Pp 577-582 (2018)
institution DOAJ
collection DOAJ
language EN
topic biodiesel
nannochloropsis
nitzschia
porong river estuary
qualitative lipid content
Biology (General)
QH301-705.5
spellingShingle biodiesel
nannochloropsis
nitzschia
porong river estuary
qualitative lipid content
Biology (General)
QH301-705.5
SRI NURHATIKA
DINI ERMAVITALINI
TRIONO BAGUS SAPUTRO
YUDI APRIYATMOKO
Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
description Nurhatika S, Ermavitalini D, Saputro TB, Apriyatmoko Y. 2018. Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia. Biodiversitas 19: 627-632. Microalgae are aquatic unicellular or multicellular microscopic photoautotroph organisms. High lipid content in microalgae biomass can be used as raw material for biodiesel. Porong river estuary is one of the sites of industrial waste disposal. The polluted waters considered will affect the lipid microalgae metabolism. The main research aims are to isolate and characterize of high lipid content microalgae found in Porong river estuary through the selection of qualitative lipid content. Sampling was conducted at three different stations. Isolation was conducted in solid agar media enriched with Porong River Estuary sterile water and using streak plate method. The isolated microalgae were characterized using a light microscope and to be matched with identification books. The lipid content was determined qualitatively by coloring the isolated-microalgae cells with Nile Red, an intracellular lipid dye then observed its luminescence color under a fluorescence microscope. Several genera of microalgae that can be isolated in this study are Oscillatoria, Nitzschia, Merismopedia, Navicula, Nannochloropsis, and Melosira. The results of qualitative lipid analysis show that the genus Nannochloropsis and Nitzschia have high intracellular lipid accumulation.
format article
author SRI NURHATIKA
DINI ERMAVITALINI
TRIONO BAGUS SAPUTRO
YUDI APRIYATMOKO
author_facet SRI NURHATIKA
DINI ERMAVITALINI
TRIONO BAGUS SAPUTRO
YUDI APRIYATMOKO
author_sort SRI NURHATIKA
title Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
title_short Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
title_full Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
title_fullStr Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
title_full_unstemmed Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
title_sort biodiversity and characterization of high lipid content microalgae in porong river estuary east java, indonesia
publisher MBI & UNS Solo
publishDate 2018
url https://doaj.org/article/4ccbcc683d404c178fa79e65ab2f5ed3
work_keys_str_mv AT srinurhatika biodiversityandcharacterizationofhighlipidcontentmicroalgaeinporongriverestuaryeastjavaindonesia
AT diniermavitalini biodiversityandcharacterizationofhighlipidcontentmicroalgaeinporongriverestuaryeastjavaindonesia
AT trionobagussaputro biodiversityandcharacterizationofhighlipidcontentmicroalgaeinporongriverestuaryeastjavaindonesia
AT yudiapriyatmoko biodiversityandcharacterizationofhighlipidcontentmicroalgaeinporongriverestuaryeastjavaindonesia
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