Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation

Abstract In kidney transplantation, microthrombi and fibrin deposition may lead to local perfusion disorders and subsequently poor initial graft function. Microthrombi are often regarded as donor-derived. However, the incidence, time of development, and potential difference between living donor kidn...

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Autores principales:	Tamar A. J. van den Berg, Marius C. van den Heuvel, Janneke Wiersema-Buist, Jelle Adelmeijer, Gertrude J. Nieuwenhuijs-Moeke, Ton Lisman, Stephan J. L. Bakker, Harry van Goor, TransplantLines Investigators, Robert A. Pol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4cce22d773de45a3b5f7036933a321cc
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spelling	oai:doaj.org-article:4cce22d773de45a3b5f7036933a321cc2021-12-02T18:48:09ZAggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation10.1038/s41598-021-97629-12045-2322https://doaj.org/article/4cce22d773de45a3b5f7036933a321cc2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97629-1https://doaj.org/toc/2045-2322Abstract In kidney transplantation, microthrombi and fibrin deposition may lead to local perfusion disorders and subsequently poor initial graft function. Microthrombi are often regarded as donor-derived. However, the incidence, time of development, and potential difference between living donor kidneys (LDK) and deceased donor kidneys(DDK), remains unclear. Two open-needle biopsies, taken at preimplantation and after reperfusion, were obtained from 17 LDK and 28 DDK transplanted between 2005 and 2008. Paraffin-embedded sections were immunohistochemically stained with anti-fibrinogen antibody. Fibrin deposition intensity in peritubular capillaries(PTC) and glomeruli was categorized as negative, weak, moderate or strong and the number of microthrombi/mm2 was quantified. Reperfusion biopsies showed more fibrin deposition (20% to 100% moderate/strong, p < 0.001) and more microthrombi/mm2 (0.97 ± 1.12 vs. 0.28 ± 0.53, p < 0.01) than preimplantation biopsies. In addition, more microthrombi/mm2 (0.38 ± 0.61 vs. 0.09 ± 0.22, p = 0.02) and stronger fibrin intensity in glomeruli (28% vs. 0%, p < 0.01) and PTC (14% vs. 0%, p = 0.02) were observed in preimplantation DDK than LDK biopsies. After reperfusion, microthrombi/mm2 were comparable (p = 0.23) for LDK (0.09 ± 0.22 to 0.76 ± 0.49, p = 0.03) and DDK (0.38 ± 0.61 to 0.90 ± 1.11, p = 0.07). Upon reperfusion, there is an aggravation of microthrombus formation and fibrin deposition within the graft. The prominent increase of microthrombi in LDK indicates that they are not merely donor-derived.Tamar A. J. van den BergMarius C. van den HeuvelJanneke Wiersema-BuistJelle AdelmeijerGertrude J. Nieuwenhuijs-MoekeTon LismanStephan J. L. BakkerHarry van GoorTransplantLines InvestigatorsRobert A. PolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tamar A. J. van den Berg Marius C. van den Heuvel Janneke Wiersema-Buist Jelle Adelmeijer Gertrude J. Nieuwenhuijs-Moeke Ton Lisman Stephan J. L. Bakker Harry van Goor TransplantLines Investigators Robert A. Pol Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
description	Abstract In kidney transplantation, microthrombi and fibrin deposition may lead to local perfusion disorders and subsequently poor initial graft function. Microthrombi are often regarded as donor-derived. However, the incidence, time of development, and potential difference between living donor kidneys (LDK) and deceased donor kidneys(DDK), remains unclear. Two open-needle biopsies, taken at preimplantation and after reperfusion, were obtained from 17 LDK and 28 DDK transplanted between 2005 and 2008. Paraffin-embedded sections were immunohistochemically stained with anti-fibrinogen antibody. Fibrin deposition intensity in peritubular capillaries(PTC) and glomeruli was categorized as negative, weak, moderate or strong and the number of microthrombi/mm2 was quantified. Reperfusion biopsies showed more fibrin deposition (20% to 100% moderate/strong, p < 0.001) and more microthrombi/mm2 (0.97 ± 1.12 vs. 0.28 ± 0.53, p < 0.01) than preimplantation biopsies. In addition, more microthrombi/mm2 (0.38 ± 0.61 vs. 0.09 ± 0.22, p = 0.02) and stronger fibrin intensity in glomeruli (28% vs. 0%, p < 0.01) and PTC (14% vs. 0%, p = 0.02) were observed in preimplantation DDK than LDK biopsies. After reperfusion, microthrombi/mm2 were comparable (p = 0.23) for LDK (0.09 ± 0.22 to 0.76 ± 0.49, p = 0.03) and DDK (0.38 ± 0.61 to 0.90 ± 1.11, p = 0.07). Upon reperfusion, there is an aggravation of microthrombus formation and fibrin deposition within the graft. The prominent increase of microthrombi in LDK indicates that they are not merely donor-derived.
format	article
author	Tamar A. J. van den Berg Marius C. van den Heuvel Janneke Wiersema-Buist Jelle Adelmeijer Gertrude J. Nieuwenhuijs-Moeke Ton Lisman Stephan J. L. Bakker Harry van Goor TransplantLines Investigators Robert A. Pol
author_facet	Tamar A. J. van den Berg Marius C. van den Heuvel Janneke Wiersema-Buist Jelle Adelmeijer Gertrude J. Nieuwenhuijs-Moeke Ton Lisman Stephan J. L. Bakker Harry van Goor TransplantLines Investigators Robert A. Pol
author_sort	Tamar A. J. van den Berg
title	Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
title_short	Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
title_full	Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
title_fullStr	Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
title_full_unstemmed	Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
title_sort	aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4cce22d773de45a3b5f7036933a321cc
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Aggravation of fibrin deposition and microthrombus formation within the graft during kidney transplantation

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