Super-resolution imaging of platelet-activation process and its quantitative analysis

Abstract Understanding the platelet activation molecular pathways by characterizing specific protein clusters within platelets is essential to identify the platelet activation state and improve the existing therapies for hemostatic disorders. Here, we employed various state-of-the-art super-resoluti...

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Autores principales:	Jinkyoung Chung, Dokyung Jeong, Geun-ho Kim, Seokran Go, Jaewoo Song, Eunyoung Moon, Yang Hoon Huh, Doory Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4cd1c5f37b954044ad60bfd9afcacd9a
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spelling	oai:doaj.org-article:4cd1c5f37b954044ad60bfd9afcacd9a2021-12-02T16:51:14ZSuper-resolution imaging of platelet-activation process and its quantitative analysis10.1038/s41598-021-89799-92045-2322https://doaj.org/article/4cd1c5f37b954044ad60bfd9afcacd9a2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89799-9https://doaj.org/toc/2045-2322Abstract Understanding the platelet activation molecular pathways by characterizing specific protein clusters within platelets is essential to identify the platelet activation state and improve the existing therapies for hemostatic disorders. Here, we employed various state-of-the-art super-resolution imaging and quantification methods to characterize the platelet spatiotemporal ultrastructural change during the activation process due to phorbol 12-myristate 13-acetate (PMA) stimuli by observing the cytoskeletal elements and various organelles at nanoscale, which cannot be done using conventional microscopy. Platelets could be spread out with the guidance of actin and microtubules, and most organelles were centralized probably due to the limited space of the peripheral thin regions or the close association with the open canalicular system (OCS). Among the centralized organelles, we provided evidence that granules are fused with the OCS to release their cargo through enlarged OCS. These findings highlight the concerted ultrastructural reorganization and relative arrangements of various organelles upon activation and call for a reassessment of previously unresolved complex and multi-factorial activation processes.Jinkyoung ChungDokyung JeongGeun-ho KimSeokran GoJaewoo SongEunyoung MoonYang Hoon HuhDoory KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinkyoung Chung Dokyung Jeong Geun-ho Kim Seokran Go Jaewoo Song Eunyoung Moon Yang Hoon Huh Doory Kim Super-resolution imaging of platelet-activation process and its quantitative analysis
description	Abstract Understanding the platelet activation molecular pathways by characterizing specific protein clusters within platelets is essential to identify the platelet activation state and improve the existing therapies for hemostatic disorders. Here, we employed various state-of-the-art super-resolution imaging and quantification methods to characterize the platelet spatiotemporal ultrastructural change during the activation process due to phorbol 12-myristate 13-acetate (PMA) stimuli by observing the cytoskeletal elements and various organelles at nanoscale, which cannot be done using conventional microscopy. Platelets could be spread out with the guidance of actin and microtubules, and most organelles were centralized probably due to the limited space of the peripheral thin regions or the close association with the open canalicular system (OCS). Among the centralized organelles, we provided evidence that granules are fused with the OCS to release their cargo through enlarged OCS. These findings highlight the concerted ultrastructural reorganization and relative arrangements of various organelles upon activation and call for a reassessment of previously unresolved complex and multi-factorial activation processes.
format	article
author	Jinkyoung Chung Dokyung Jeong Geun-ho Kim Seokran Go Jaewoo Song Eunyoung Moon Yang Hoon Huh Doory Kim
author_facet	Jinkyoung Chung Dokyung Jeong Geun-ho Kim Seokran Go Jaewoo Song Eunyoung Moon Yang Hoon Huh Doory Kim
author_sort	Jinkyoung Chung
title	Super-resolution imaging of platelet-activation process and its quantitative analysis
title_short	Super-resolution imaging of platelet-activation process and its quantitative analysis
title_full	Super-resolution imaging of platelet-activation process and its quantitative analysis
title_fullStr	Super-resolution imaging of platelet-activation process and its quantitative analysis
title_full_unstemmed	Super-resolution imaging of platelet-activation process and its quantitative analysis
title_sort	super-resolution imaging of platelet-activation process and its quantitative analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4cd1c5f37b954044ad60bfd9afcacd9a
work_keys_str_mv	AT jinkyoungchung superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT dokyungjeong superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT geunhokim superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT seokrango superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT jaewoosong superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT eunyoungmoon superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT yanghoonhuh superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis AT doorykim superresolutionimagingofplateletactivationprocessanditsquantitativeanalysis
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Super-resolution imaging of platelet-activation process and its quantitative analysis

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