Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing.

Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed the Rare Biosphere. Multiple studies have inves...

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Bibliographic Details
Main Authors: Charles K Lee, Craig W Herbold, Shawn W Polson, K Eric Wommack, Shannon J Williamson, Ian R McDonald, S Craig Cary
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2012
Subjects:
R
Q
Online Access:https://doaj.org/article/4cd79a666d4a406e823c45e048c8e514
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