Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model

Rixiong Wang,1,2 Hui Liu,3,4 Jun Song,3,4 Qing Wu1,2 1Department of Oncology, the First Affiliated Hospital of Fujian Medical University, Fuzhou City, People’s Republic of China; 2Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital of Fujian Medical University, Fuzh...

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Autores principales:	Wang R, Liu H, Song J, Wu Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	gastric cancer melatonin growth angiogenesis vascular endothelial growth factor angiogenin chick embryo tumor xenograft model Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/4cda28cbbaf64ba18b9912b895706c1c
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spelling	oai:doaj.org-article:4cda28cbbaf64ba18b9912b895706c1c2021-11-23T18:43:01ZActivity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model1179-1322https://doaj.org/article/4cda28cbbaf64ba18b9912b895706c1c2021-11-01T00:00:00Zhttps://www.dovepress.com/activity-of-melatonin-against-gastric-cancer-growth-in-a-chick-embryo--peer-reviewed-fulltext-article-CMARhttps://doaj.org/toc/1179-1322Rixiong Wang,1,2 Hui Liu,3,4 Jun Song,3,4 Qing Wu1,2 1Department of Oncology, the First Affiliated Hospital of Fujian Medical University, Fuzhou City, People’s Republic of China; 2Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital of Fujian Medical University, Fuzhou City, People’s Republic of China; 3Department of Human Anatomy, Histology and Embryology, Fujian Medical University, Fuzhou City, People’s Republic of China; 4Key Laboratory of the Ministry of Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou City, People’s Republic of ChinaCorrespondence: Rixiong Wang Email 13960758357@fjmu.edu.cnPurpose: Previous studies have shown the antitumor activity of melatonin against a wide range of human cancers; however, the impact of melatonin on gastric cancer growth remains to be illustrated. This study aimed to investigate the activity of melatonin against gastric cancer growth in a chick embryo tumor xenograft model and explore the possible mechanisms.Materials and Methods: The growth of gastric cancer SGC-7901 cells was measured using MTT assay, and a chick embryo tumor xenograft model was generated to observe the effect of melatonin on gastric cancer growth in vivo. In addition, the VEGF and angiogenin secretion was measured in the supernatant of chick embryo tumor xenograft models with ELISA.Results: MLT treatment inhibited the growth of SGC-7901 cells at a concentration-dependent manner, and treatment with MLT at 1 mM was found to markedly reduce the volume and weight of tumors bearing the allantois of chicken embryos. ELISA showed that MLT at concentrations of 0.0041, 0.012, 0.037 and 0.11 had no remarkable impact on VEGF and angiopoietin secretion, while MLT at 1 mM significantly suppressed VEGF and angiopoietin production in chick embryo tumor xenograft models with SGC-7901 cells (P = 0.023).Conclusion: Our data demonstrate that MLT inhibits gastric cancer growth in vitro at a concentration-dependent manner, and suppresses angiogenesis of the chick embryo tumor xenograft model with SGC-7901 cells through inhibiting VEGF and angiogenin secretion. Further studies are needed to investigate the therapeutic potential of MLT for gastric cancer as compared to drugs clinically approved.Keywords: gastric cancer, melatonin, growth, angiogenesis, vascular endothelial growth factor, angiogenin, chick embryo tumor xenograft modelWang RLiu HSong JWu QDove Medical Pressarticlegastric cancermelatoningrowthangiogenesisvascular endothelial growth factorangiogeninchick embryo tumor xenograft modelNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Management and Research, Vol Volume 13, Pp 8803-8808 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastric cancer melatonin growth angiogenesis vascular endothelial growth factor angiogenin chick embryo tumor xenograft model Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	gastric cancer melatonin growth angiogenesis vascular endothelial growth factor angiogenin chick embryo tumor xenograft model Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Wang R Liu H Song J Wu Q Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model
description	Rixiong Wang,1,2 Hui Liu,3,4 Jun Song,3,4 Qing Wu1,2 1Department of Oncology, the First Affiliated Hospital of Fujian Medical University, Fuzhou City, People’s Republic of China; 2Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital of Fujian Medical University, Fuzhou City, People’s Republic of China; 3Department of Human Anatomy, Histology and Embryology, Fujian Medical University, Fuzhou City, People’s Republic of China; 4Key Laboratory of the Ministry of Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou City, People’s Republic of ChinaCorrespondence: Rixiong Wang Email 13960758357@fjmu.edu.cnPurpose: Previous studies have shown the antitumor activity of melatonin against a wide range of human cancers; however, the impact of melatonin on gastric cancer growth remains to be illustrated. This study aimed to investigate the activity of melatonin against gastric cancer growth in a chick embryo tumor xenograft model and explore the possible mechanisms.Materials and Methods: The growth of gastric cancer SGC-7901 cells was measured using MTT assay, and a chick embryo tumor xenograft model was generated to observe the effect of melatonin on gastric cancer growth in vivo. In addition, the VEGF and angiogenin secretion was measured in the supernatant of chick embryo tumor xenograft models with ELISA.Results: MLT treatment inhibited the growth of SGC-7901 cells at a concentration-dependent manner, and treatment with MLT at 1 mM was found to markedly reduce the volume and weight of tumors bearing the allantois of chicken embryos. ELISA showed that MLT at concentrations of 0.0041, 0.012, 0.037 and 0.11 had no remarkable impact on VEGF and angiopoietin secretion, while MLT at 1 mM significantly suppressed VEGF and angiopoietin production in chick embryo tumor xenograft models with SGC-7901 cells (P = 0.023).Conclusion: Our data demonstrate that MLT inhibits gastric cancer growth in vitro at a concentration-dependent manner, and suppresses angiogenesis of the chick embryo tumor xenograft model with SGC-7901 cells through inhibiting VEGF and angiogenin secretion. Further studies are needed to investigate the therapeutic potential of MLT for gastric cancer as compared to drugs clinically approved.Keywords: gastric cancer, melatonin, growth, angiogenesis, vascular endothelial growth factor, angiogenin, chick embryo tumor xenograft model
format	article
author	Wang R Liu H Song J Wu Q
author_facet	Wang R Liu H Song J Wu Q
author_sort	Wang R
title	Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model
title_short	Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model
title_full	Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model
title_fullStr	Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model
title_full_unstemmed	Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model
title_sort	activity of melatonin against gastric cancer growth in a chick embryo tumor xenograft model
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/4cda28cbbaf64ba18b9912b895706c1c
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Activity of Melatonin Against Gastric Cancer Growth in a Chick Embryo Tumor Xenograft Model

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