Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.

The rind appearance of melon is one of the most vital commercial quality traits which determines the preferences and behavior of consumers toward the consumption of melon. In this study, we constructed an F2 population derived from SC (mottled rind) and MG (non-mottled rind) lines for mapping the mo...

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Autores principales:	Jia Shen, Xinyang Xu, Yuejian Zhang, Xiaowei Niu, Weisong Shou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	melon mottled rind spots chlorophyll mapping Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/4cdaa2676b314d25b9aa9bb7f2b9fbf6
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spelling	oai:doaj.org-article:4cdaa2676b314d25b9aa9bb7f2b9fbf62021-11-15T06:32:22ZGenetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.1664-462X10.3389/fpls.2021.769989https://doaj.org/article/4cdaa2676b314d25b9aa9bb7f2b9fbf62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.769989/fullhttps://doaj.org/toc/1664-462XThe rind appearance of melon is one of the most vital commercial quality traits which determines the preferences and behavior of consumers toward the consumption of melon. In this study, we constructed an F2 population derived from SC (mottled rind) and MG (non-mottled rind) lines for mapping the mottled rind gene(s) in melon. Genetic analysis showed that there were two dominant genes (CmMt1 and CmMt2) with evidence of epistasis controlling the mottled rind. Meanwhile, the phenotypic segregation ratio implied that the immature rind color had an epistatic effect on the mottled rind, which was regulated by CmAPRR2. A Kompetitive Allele-Specific PCR (KASP) DNA marker (CmAPRR2SNP(G/T)) was developed and shown to co-segregate with rind color, confirming that CmAPRR2 was CmMt1. Using bulked segregant analysis sequencing and KASP assays, CmMt2 was fine-mapped to an interval of 40.6 kb with six predicted genes. Functional annotation, expression analysis, and sequence variation analyses confirmed that AtCPSFL1 homolog, MELO3C026282, was the most likely candidate gene for CmMt2. Moreover, pigment content measurement and transmission electron microscopy analysis demonstrated that CmMt2 might participate in the development of chloroplast, which, in turn, decreases the accumulation of chlorophyll. These results provide insight into the molecular mechanism underlying rind appearance and reveal valuable information for marker-assisted selection breeding in melon.Jia ShenXinyang XuYuejian ZhangXiaowei NiuWeisong ShouFrontiers Media S.A.articlemelonmottled rindspotschlorophyllmappingPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melon mottled rind spots chlorophyll mapping Plant culture SB1-1110
spellingShingle	melon mottled rind spots chlorophyll mapping Plant culture SB1-1110 Jia Shen Xinyang Xu Yuejian Zhang Xiaowei Niu Weisong Shou Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.
description	The rind appearance of melon is one of the most vital commercial quality traits which determines the preferences and behavior of consumers toward the consumption of melon. In this study, we constructed an F2 population derived from SC (mottled rind) and MG (non-mottled rind) lines for mapping the mottled rind gene(s) in melon. Genetic analysis showed that there were two dominant genes (CmMt1 and CmMt2) with evidence of epistasis controlling the mottled rind. Meanwhile, the phenotypic segregation ratio implied that the immature rind color had an epistatic effect on the mottled rind, which was regulated by CmAPRR2. A Kompetitive Allele-Specific PCR (KASP) DNA marker (CmAPRR2SNP(G/T)) was developed and shown to co-segregate with rind color, confirming that CmAPRR2 was CmMt1. Using bulked segregant analysis sequencing and KASP assays, CmMt2 was fine-mapped to an interval of 40.6 kb with six predicted genes. Functional annotation, expression analysis, and sequence variation analyses confirmed that AtCPSFL1 homolog, MELO3C026282, was the most likely candidate gene for CmMt2. Moreover, pigment content measurement and transmission electron microscopy analysis demonstrated that CmMt2 might participate in the development of chloroplast, which, in turn, decreases the accumulation of chlorophyll. These results provide insight into the molecular mechanism underlying rind appearance and reveal valuable information for marker-assisted selection breeding in melon.
format	article
author	Jia Shen Xinyang Xu Yuejian Zhang Xiaowei Niu Weisong Shou
author_facet	Jia Shen Xinyang Xu Yuejian Zhang Xiaowei Niu Weisong Shou
author_sort	Jia Shen
title	Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.
title_short	Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.
title_full	Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.
title_fullStr	Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.
title_full_unstemmed	Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.
title_sort	genetic mapping and identification of the candidate genes for mottled rind in cucumis melo l.
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4cdaa2676b314d25b9aa9bb7f2b9fbf6
work_keys_str_mv	AT jiashen geneticmappingandidentificationofthecandidategenesformottledrindincucumismelol AT xinyangxu geneticmappingandidentificationofthecandidategenesformottledrindincucumismelol AT yuejianzhang geneticmappingandidentificationofthecandidategenesformottledrindincucumismelol AT xiaoweiniu geneticmappingandidentificationofthecandidategenesformottledrindincucumismelol AT weisongshou geneticmappingandidentificationofthecandidategenesformottledrindincucumismelol
_version_	1718428550919356416

Genetic Mapping and Identification of the Candidate Genes for Mottled Rind in Cucumis melo L.

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