Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands

Abstract Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang Xu, Lingmo Chang, Anliang Huang, Xiaojun Liu, Xinyu Liu, Hong Zhou, Joshua G. Liang, Peng Liang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4cdf3414717f4446a182075804eda027
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4cdf3414717f4446a182075804eda027
record_format	dspace
spelling	oai:doaj.org-article:4cdf3414717f4446a182075804eda0272021-12-02T15:05:54ZFunctional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands10.1038/s41598-017-06343-42045-2322https://doaj.org/article/4cdf3414717f4446a182075804eda0272017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06343-4https://doaj.org/toc/2045-2322Abstract Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression.Yang XuLingmo ChangAnliang HuangXiaojun LiuXinyu LiuHong ZhouJoshua G. LiangPeng LiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Xu Lingmo Chang Anliang Huang Xiaojun Liu Xinyu Liu Hong Zhou Joshua G. Liang Peng Liang Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
description	Abstract Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression.
format	article
author	Yang Xu Lingmo Chang Anliang Huang Xiaojun Liu Xinyu Liu Hong Zhou Joshua G. Liang Peng Liang
author_facet	Yang Xu Lingmo Chang Anliang Huang Xiaojun Liu Xinyu Liu Hong Zhou Joshua G. Liang Peng Liang
author_sort	Yang Xu
title	Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_short	Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_full	Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_fullStr	Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_full_unstemmed	Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands
title_sort	functional detection of tnf receptor family members by affinity-labeled ligands
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4cdf3414717f4446a182075804eda027
work_keys_str_mv	AT yangxu functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT lingmochang functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT anlianghuang functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT xiaojunliu functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT xinyuliu functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT hongzhou functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT joshuagliang functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands AT pengliang functionaldetectionoftnfreceptorfamilymembersbyaffinitylabeledligands
_version_	1718388642247868416

Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands

Ejemplares similares