Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing
Abstract Cardiac tissue engineering, which combines cells and supportive scaffolds, is an emerging treatment for restoring cardiac function after myocardial infarction (MI), although, the optimal construct remains a challenge. We developed two engineered cardiac grafts, based on decellularized scaff...
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Nature Portfolio
2018
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oai:doaj.org-article:4ce7e5a2aff842ad94cdf5df21a4e19d2021-12-02T12:32:21ZHead-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing10.1038/s41598-018-25115-22045-2322https://doaj.org/article/4ce7e5a2aff842ad94cdf5df21a4e19d2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25115-2https://doaj.org/toc/2045-2322Abstract Cardiac tissue engineering, which combines cells and supportive scaffolds, is an emerging treatment for restoring cardiac function after myocardial infarction (MI), although, the optimal construct remains a challenge. We developed two engineered cardiac grafts, based on decellularized scaffolds from myocardial and pericardial tissues and repopulated them with adipose tissue mesenchymal stem cells (ATMSCs). The structure, macromechanical and micromechanical scaffold properties were preserved upon the decellularization and recellularization processes, except for recellularized myocardium micromechanics that was ∼2-fold stiffer than native tissue and decellularized scaffolds. Proteome characterization of the two acellular matrices showed enrichment of matrisome proteins and major cardiac extracellular matrix components, considerably higher for the recellularized pericardium. Moreover, the pericardial scaffold demonstrated better cell penetrance and retention, as well as a bigger pore size. Both engineered cardiac grafts were further evaluated in pre-clinical MI swine models. Forty days after graft implantation, swine treated with the engineered cardiac grafts showed significant ventricular function recovery. Irrespective of the scaffold origin or cell recolonization, all scaffolds integrated with the underlying myocardium and showed signs of neovascularization and nerve sprouting. Collectively, engineered cardiac grafts -with pericardial or myocardial scaffolds- were effective in restoring cardiac function post-MI, and pericardial scaffolds showed better structural integrity and recolonization capability.Isaac Perea-GilCarolina Gálvez-MontónCristina Prat-VidalIgnasi JorbaCristina Segú-VergésSantiago RouraCarolina Soler-BotijaOriol Iborra-EgeaElena Revuelta-LópezMarco A. FernándezRamon FarréDaniel NavajasAntoni Bayes-GenisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
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Medicine R Science Q Isaac Perea-Gil Carolina Gálvez-Montón Cristina Prat-Vidal Ignasi Jorba Cristina Segú-Vergés Santiago Roura Carolina Soler-Botija Oriol Iborra-Egea Elena Revuelta-López Marco A. Fernández Ramon Farré Daniel Navajas Antoni Bayes-Genis Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing |
| description |
Abstract Cardiac tissue engineering, which combines cells and supportive scaffolds, is an emerging treatment for restoring cardiac function after myocardial infarction (MI), although, the optimal construct remains a challenge. We developed two engineered cardiac grafts, based on decellularized scaffolds from myocardial and pericardial tissues and repopulated them with adipose tissue mesenchymal stem cells (ATMSCs). The structure, macromechanical and micromechanical scaffold properties were preserved upon the decellularization and recellularization processes, except for recellularized myocardium micromechanics that was ∼2-fold stiffer than native tissue and decellularized scaffolds. Proteome characterization of the two acellular matrices showed enrichment of matrisome proteins and major cardiac extracellular matrix components, considerably higher for the recellularized pericardium. Moreover, the pericardial scaffold demonstrated better cell penetrance and retention, as well as a bigger pore size. Both engineered cardiac grafts were further evaluated in pre-clinical MI swine models. Forty days after graft implantation, swine treated with the engineered cardiac grafts showed significant ventricular function recovery. Irrespective of the scaffold origin or cell recolonization, all scaffolds integrated with the underlying myocardium and showed signs of neovascularization and nerve sprouting. Collectively, engineered cardiac grafts -with pericardial or myocardial scaffolds- were effective in restoring cardiac function post-MI, and pericardial scaffolds showed better structural integrity and recolonization capability. |
| format |
article |
| author |
Isaac Perea-Gil Carolina Gálvez-Montón Cristina Prat-Vidal Ignasi Jorba Cristina Segú-Vergés Santiago Roura Carolina Soler-Botija Oriol Iborra-Egea Elena Revuelta-López Marco A. Fernández Ramon Farré Daniel Navajas Antoni Bayes-Genis |
| author_facet |
Isaac Perea-Gil Carolina Gálvez-Montón Cristina Prat-Vidal Ignasi Jorba Cristina Segú-Vergés Santiago Roura Carolina Soler-Botija Oriol Iborra-Egea Elena Revuelta-López Marco A. Fernández Ramon Farré Daniel Navajas Antoni Bayes-Genis |
| author_sort |
Isaac Perea-Gil |
| title |
Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing |
| title_short |
Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing |
| title_full |
Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing |
| title_fullStr |
Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing |
| title_full_unstemmed |
Head-to-head comparison of two engineered cardiac grafts for myocardial repair: From scaffold characterization to pre-clinical testing |
| title_sort |
head-to-head comparison of two engineered cardiac grafts for myocardial repair: from scaffold characterization to pre-clinical testing |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/4ce7e5a2aff842ad94cdf5df21a4e19d |
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