A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.

<h4>Aims</h4>To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice.<h4>Methods</h4>C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease in...

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Autores principales: Juliana D Lourenço, Luana P Neves, Clarice R Olivo, Adriana Duran, Francine M Almeida, Petra M M Arantes, Carla M Prado, Edna Aparecida Leick, Aparecida S Tanaka, Mílton A Martins, Sergio D Sasaki, Fernanda D T Q S Lopes
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spelling oai:doaj.org-article:4ceb7bc33a3643979cf45e3e658d440e2021-11-18T08:17:43ZA treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.1932-620310.1371/journal.pone.0098216https://doaj.org/article/4ceb7bc33a3643979cf45e3e658d440e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24886716/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aims</h4>To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice.<h4>Methods</h4>C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease inhibitor (rBmTI-A) before (Protocol 1) and after (Protocol 2) emphysema development. In both protocols, we evaluated lung function to evaluate the airway resistance (Raw), tissue damping (Gtis) and tissue elastance (Htis). The inflammatory profile was analyzed in the bronchoalveolar lavage (BALF) and through the use of morphometry; we measured the mean linear intercept (Lm) (to verify alveolar enlargement), the volume proportion of collagen and elastic fibers, and the numbers of macrophages and metalloprotease 12 (MMP-12) positive cells in the parenchyma. We showed that at both time points, even after the emphysema was established, the rBmTI-A treatment was sufficient to reverse the loss of elastic recoil measured by Htis, the alveolar enlargement and the increase in the total number of cells in the BALF, with a primary decrease in the number of macrophages. Although, the treatment did not control the increase in macrophages in the lung parenchyma, it was sufficient to decrease the number of positive cells for MMP-12 and reduce the volume of collagen fibers, which was increased in PPE groups. These findings attest to the importance of MMP-12 in PPE-induced emphysema and suggest that this metalloprotease could be an effective therapeutic target.Juliana D LourençoLuana P NevesClarice R OlivoAdriana DuranFrancine M AlmeidaPetra M M ArantesCarla M PradoEdna Aparecida LeickAparecida S TanakaMílton A MartinsSergio D SasakiFernanda D T Q S LopesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98216 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Juliana D Lourenço
Luana P Neves
Clarice R Olivo
Adriana Duran
Francine M Almeida
Petra M M Arantes
Carla M Prado
Edna Aparecida Leick
Aparecida S Tanaka
Mílton A Martins
Sergio D Sasaki
Fernanda D T Q S Lopes
A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.
description <h4>Aims</h4>To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice.<h4>Methods</h4>C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease inhibitor (rBmTI-A) before (Protocol 1) and after (Protocol 2) emphysema development. In both protocols, we evaluated lung function to evaluate the airway resistance (Raw), tissue damping (Gtis) and tissue elastance (Htis). The inflammatory profile was analyzed in the bronchoalveolar lavage (BALF) and through the use of morphometry; we measured the mean linear intercept (Lm) (to verify alveolar enlargement), the volume proportion of collagen and elastic fibers, and the numbers of macrophages and metalloprotease 12 (MMP-12) positive cells in the parenchyma. We showed that at both time points, even after the emphysema was established, the rBmTI-A treatment was sufficient to reverse the loss of elastic recoil measured by Htis, the alveolar enlargement and the increase in the total number of cells in the BALF, with a primary decrease in the number of macrophages. Although, the treatment did not control the increase in macrophages in the lung parenchyma, it was sufficient to decrease the number of positive cells for MMP-12 and reduce the volume of collagen fibers, which was increased in PPE groups. These findings attest to the importance of MMP-12 in PPE-induced emphysema and suggest that this metalloprotease could be an effective therapeutic target.
format article
author Juliana D Lourenço
Luana P Neves
Clarice R Olivo
Adriana Duran
Francine M Almeida
Petra M M Arantes
Carla M Prado
Edna Aparecida Leick
Aparecida S Tanaka
Mílton A Martins
Sergio D Sasaki
Fernanda D T Q S Lopes
author_facet Juliana D Lourenço
Luana P Neves
Clarice R Olivo
Adriana Duran
Francine M Almeida
Petra M M Arantes
Carla M Prado
Edna Aparecida Leick
Aparecida S Tanaka
Mílton A Martins
Sergio D Sasaki
Fernanda D T Q S Lopes
author_sort Juliana D Lourenço
title A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.
title_short A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.
title_full A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.
title_fullStr A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.
title_full_unstemmed A treatment with a protease inhibitor recombinant from the cattle tick (Rhipicephalus Boophilus microplus) ameliorates emphysema in mice.
title_sort treatment with a protease inhibitor recombinant from the cattle tick (rhipicephalus boophilus microplus) ameliorates emphysema in mice.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/4ceb7bc33a3643979cf45e3e658d440e
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