Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.

<h4>Purpose</h4>Pluripotent cells residing in the periosteum, a bi-layered membrane enveloping all bones, exhibit a remarkable regenerative capacity to fill in critical sized defects of the ovine femur within two weeks of treatment. Harnessing the regenerative power of the periosteum app...

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Autores principales:	Melissa L Knothe Tate, Hana Chang, Shannon R Moore, Ulf R Knothe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4cf10087662b4baca8dcb9303da66801
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spelling	oai:doaj.org-article:4cf10087662b4baca8dcb9303da668012021-11-18T07:32:31ZSurgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.1932-620310.1371/journal.pone.0028702https://doaj.org/article/4cf10087662b4baca8dcb9303da668012011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174873/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>Pluripotent cells residing in the periosteum, a bi-layered membrane enveloping all bones, exhibit a remarkable regenerative capacity to fill in critical sized defects of the ovine femur within two weeks of treatment. Harnessing the regenerative power of the periosteum appears to be limited only by the amount of healthy periosteum available. Here we use a substitute periosteum, a delivery device cum implant, to test the hypothesis that directional delivery of endogenous periosteal factors enhances bone defect healing.<h4>Methods</h4>Newly adapted surgical protocols were used to create critical sized, middiaphyseal femur defects in four groups of five skeletally mature Swiss alpine sheep. Each group was treated using a periosteum substitute for the controlled addition of periosteal factors including the presence of collagen in the periosteum (Group 1), periosteum derived cells (Group 2), and autogenic periosteal strips (Group 3). Control group animals were treated with an isotropic elastomer membrane alone. We hypothesized that periosteal substitute membranes incorporating the most periosteal factors would show superior defect infilling compared to substitute membranes integrating fewer factors (i.e. Group 3>Group 2>Group 1>Control).<h4>Results</h4>Based on micro-computed tomography data, bone defects enveloped by substitute periosteum enabling directional delivery of periosteal factors exhibit superior bony bridging compared to those sheathed with isotropic membrane controls (Group 3>Group 2>Group 1, Control). Quantitative histological analysis shows significantly increased de novo tissue generation with delivery of periosteal factors, compared to the substitute periosteum containing a collagen membrane alone (Group 1) as well as compared to the isotropic control membrane. Greatest tissue generation and maximal defect bridging was observed when autologous periosteal transplant strips were included in the periosteum substitute.<h4>Conclusion</h4>Periosteum-derived cells as well as other factors intrinsic to periosteum play a key role for infilling of critical sized defects.Melissa L Knothe TateHana ChangShannon R MooreUlf R KnothePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28702 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melissa L Knothe Tate Hana Chang Shannon R Moore Ulf R Knothe Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
description	<h4>Purpose</h4>Pluripotent cells residing in the periosteum, a bi-layered membrane enveloping all bones, exhibit a remarkable regenerative capacity to fill in critical sized defects of the ovine femur within two weeks of treatment. Harnessing the regenerative power of the periosteum appears to be limited only by the amount of healthy periosteum available. Here we use a substitute periosteum, a delivery device cum implant, to test the hypothesis that directional delivery of endogenous periosteal factors enhances bone defect healing.<h4>Methods</h4>Newly adapted surgical protocols were used to create critical sized, middiaphyseal femur defects in four groups of five skeletally mature Swiss alpine sheep. Each group was treated using a periosteum substitute for the controlled addition of periosteal factors including the presence of collagen in the periosteum (Group 1), periosteum derived cells (Group 2), and autogenic periosteal strips (Group 3). Control group animals were treated with an isotropic elastomer membrane alone. We hypothesized that periosteal substitute membranes incorporating the most periosteal factors would show superior defect infilling compared to substitute membranes integrating fewer factors (i.e. Group 3>Group 2>Group 1>Control).<h4>Results</h4>Based on micro-computed tomography data, bone defects enveloped by substitute periosteum enabling directional delivery of periosteal factors exhibit superior bony bridging compared to those sheathed with isotropic membrane controls (Group 3>Group 2>Group 1, Control). Quantitative histological analysis shows significantly increased de novo tissue generation with delivery of periosteal factors, compared to the substitute periosteum containing a collagen membrane alone (Group 1) as well as compared to the isotropic control membrane. Greatest tissue generation and maximal defect bridging was observed when autologous periosteal transplant strips were included in the periosteum substitute.<h4>Conclusion</h4>Periosteum-derived cells as well as other factors intrinsic to periosteum play a key role for infilling of critical sized defects.
format	article
author	Melissa L Knothe Tate Hana Chang Shannon R Moore Ulf R Knothe
author_facet	Melissa L Knothe Tate Hana Chang Shannon R Moore Ulf R Knothe
author_sort	Melissa L Knothe Tate
title	Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
title_short	Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
title_full	Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
title_fullStr	Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
title_full_unstemmed	Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
title_sort	surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4cf10087662b4baca8dcb9303da66801
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Surgical membranes as directional delivery devices to generate tissue: testing in an ovine critical sized defect model.

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