Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles

Currently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38.0 million people were living with HIV in 2019, only 68% of HIV-infected individuals were accessing...

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Autores principales:	Yoshiki Eto, Narcís Saubi, Pau Ferrer, Joan Joseph-Munné
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HPV HIV virus-like particle chimeric vaccine yeast Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/4cf6175832534e679a66443b6624a38f
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spelling	oai:doaj.org-article:4cf6175832534e679a66443b6624a38f2021-11-25T18:42:28ZExpression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles10.3390/pharmaceutics131119671999-4923https://doaj.org/article/4cf6175832534e679a66443b6624a38f2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1967https://doaj.org/toc/1999-4923Currently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38.0 million people were living with HIV in 2019, only 68% of HIV-infected individuals were accessing antiretroviral therapy as of the end of June 2020 and there is no HIV vaccine yet. Therefore, safe, effective, and affordable vaccines against those two viruses are immediately needed. Both HPV and HIV are sexually transmitted infections and one of the main access routes is the mucosal genital tract. Thus, the development of a combined vaccine that would protect against HPV and HIV infections is a logical effort in the fight against these two major global pathogens. In this study, a recombinant <i>Pichia pastoris</i> producing chimeric HPV-HIV L1P18 protein intracellularly was constructed. After cell disruption, the supernatant was collected, and the VLPs were purified by a combination of ammonium sulfate precipitation, size exclusion chromatography, ultracentrifugation, and ultrafiltration. At the end of purification process, the chimeric VLPs were recovered with 96% purity and 9.23% overall yield, and the morphology of VLPs were confirmed by transmission electron microscopy. This work contributes towards the development of an alternative platform for production of a bivalent vaccine against HPV and HIV in <i>P. pastoris</i>.Yoshiki EtoNarcís SaubiPau FerrerJoan Joseph-MunnéMDPI AGarticleHPVHIVvirus-like particlechimericvaccineyeastPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1967, p 1967 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HPV HIV virus-like particle chimeric vaccine yeast Pharmacy and materia medica RS1-441
spellingShingle	HPV HIV virus-like particle chimeric vaccine yeast Pharmacy and materia medica RS1-441 Yoshiki Eto Narcís Saubi Pau Ferrer Joan Joseph-Munné Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles
description	Currently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38.0 million people were living with HIV in 2019, only 68% of HIV-infected individuals were accessing antiretroviral therapy as of the end of June 2020 and there is no HIV vaccine yet. Therefore, safe, effective, and affordable vaccines against those two viruses are immediately needed. Both HPV and HIV are sexually transmitted infections and one of the main access routes is the mucosal genital tract. Thus, the development of a combined vaccine that would protect against HPV and HIV infections is a logical effort in the fight against these two major global pathogens. In this study, a recombinant <i>Pichia pastoris</i> producing chimeric HPV-HIV L1P18 protein intracellularly was constructed. After cell disruption, the supernatant was collected, and the VLPs were purified by a combination of ammonium sulfate precipitation, size exclusion chromatography, ultracentrifugation, and ultrafiltration. At the end of purification process, the chimeric VLPs were recovered with 96% purity and 9.23% overall yield, and the morphology of VLPs were confirmed by transmission electron microscopy. This work contributes towards the development of an alternative platform for production of a bivalent vaccine against HPV and HIV in <i>P. pastoris</i>.
format	article
author	Yoshiki Eto Narcís Saubi Pau Ferrer Joan Joseph-Munné
author_facet	Yoshiki Eto Narcís Saubi Pau Ferrer Joan Joseph-Munné
author_sort	Yoshiki Eto
title	Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles
title_short	Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles
title_full	Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles
title_fullStr	Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles
title_full_unstemmed	Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles
title_sort	expression of chimeric hpv-hiv protein l1p18 in <i>pichia pastoris</i>; purification and characterization of the virus-like particles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4cf6175832534e679a66443b6624a38f
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Expression of Chimeric HPV-HIV Protein L1P18 in <i>Pichia pastoris</i>; Purification and Characterization of the Virus-like Particles

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