A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion

Abstract Microfluidic chemical gradient generators enable precise spatiotemporal control of chemotactic signals to study cellular behavior with high resolution and reliability. However, time and cost consuming preparation steps for cell adhesion in microchannels as well as requirement of pumping fac...

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Autores principales:	Mohamadmahdi Samandari, Laleh Rafiee, Fatemeh Alipanah, Amir Sanati-Nezhad, Shaghayegh Haghjooy Javanmard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4cfb1b82b8564b549482876ea6895df6
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spelling	oai:doaj.org-article:4cfb1b82b8564b549482876ea6895df62021-12-02T17:16:10ZA simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion10.1038/s41598-021-89635-02045-2322https://doaj.org/article/4cfb1b82b8564b549482876ea6895df62021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89635-0https://doaj.org/toc/2045-2322Abstract Microfluidic chemical gradient generators enable precise spatiotemporal control of chemotactic signals to study cellular behavior with high resolution and reliability. However, time and cost consuming preparation steps for cell adhesion in microchannels as well as requirement of pumping facilities usually complicate the application of the microfluidic assays. Here, we introduce a simple strategy for preparation of a reusable and stand-alone microfluidic gradient generator to study cellular behavior. Polydimethylsiloxane (PDMS) is directly mounted on the commercial polystyrene-based cell culture surfaces by manipulating the PDMS curing time to optimize bonding strength. The stand-alone strategy not only offers pumpless application of this microfluidic device but also ensures minimal fluidic pressure and consequently a leakage-free system. Elimination of any surface treatment or coating significantly facilitates the preparation of the microfluidic assay and offers a detachable PDMS microchip which can be reused following to a simple cleaning and sterilization step. The chemotactic signal in our microchip is further characterized using numerical and experimental evaluations and it is demonstrated that the device can generate both linear and polynomial signals. Finally, the feasibility of the strategy in deciphering cellular behavior is demonstrated by exploring cancer cell migration and invasion in response to chemical stimuli. The introduced strategy can significantly decrease the complexity of the microfluidic chemotaxis assays and increase their throughput for various cellular and molecular studies.Mohamadmahdi SamandariLaleh RafieeFatemeh AlipanahAmir Sanati-NezhadShaghayegh Haghjooy JavanmardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohamadmahdi Samandari Laleh Rafiee Fatemeh Alipanah Amir Sanati-Nezhad Shaghayegh Haghjooy Javanmard A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
description	Abstract Microfluidic chemical gradient generators enable precise spatiotemporal control of chemotactic signals to study cellular behavior with high resolution and reliability. However, time and cost consuming preparation steps for cell adhesion in microchannels as well as requirement of pumping facilities usually complicate the application of the microfluidic assays. Here, we introduce a simple strategy for preparation of a reusable and stand-alone microfluidic gradient generator to study cellular behavior. Polydimethylsiloxane (PDMS) is directly mounted on the commercial polystyrene-based cell culture surfaces by manipulating the PDMS curing time to optimize bonding strength. The stand-alone strategy not only offers pumpless application of this microfluidic device but also ensures minimal fluidic pressure and consequently a leakage-free system. Elimination of any surface treatment or coating significantly facilitates the preparation of the microfluidic assay and offers a detachable PDMS microchip which can be reused following to a simple cleaning and sterilization step. The chemotactic signal in our microchip is further characterized using numerical and experimental evaluations and it is demonstrated that the device can generate both linear and polynomial signals. Finally, the feasibility of the strategy in deciphering cellular behavior is demonstrated by exploring cancer cell migration and invasion in response to chemical stimuli. The introduced strategy can significantly decrease the complexity of the microfluidic chemotaxis assays and increase their throughput for various cellular and molecular studies.
format	article
author	Mohamadmahdi Samandari Laleh Rafiee Fatemeh Alipanah Amir Sanati-Nezhad Shaghayegh Haghjooy Javanmard
author_facet	Mohamadmahdi Samandari Laleh Rafiee Fatemeh Alipanah Amir Sanati-Nezhad Shaghayegh Haghjooy Javanmard
author_sort	Mohamadmahdi Samandari
title	A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
title_short	A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
title_full	A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
title_fullStr	A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
title_full_unstemmed	A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
title_sort	simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4cfb1b82b8564b549482876ea6895df6
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A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion

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