β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?

As urine is not sterile, inflammatory reactions caused by dysbiosis of the urinary microbiota may induce interstitial cystitis. A study was conducted to determine whether β-defensin 2 (BD-2), a specific antimicrobial peptide in the bladder, could be used as a novel diagnostic marker for ulcerative i...

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Autores principales:	Sang Wook Lee, Si Hyun Kim, Kwang Woo Lee, Woong Bin Kim, Hae Woong Choi, Ji Eun Moon, Ahrim Moon, Young Ho Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	interstitial cystitis biomarkers urine specimen collection antimicrobial peptide beta-defensins Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4d0329e8824344b5a12ba4d36a910c04
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spelling	oai:doaj.org-article:4d0329e8824344b5a12ba4d36a910c042021-11-25T17:21:28Zβ-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?10.3390/diagnostics111120822075-4418https://doaj.org/article/4d0329e8824344b5a12ba4d36a910c042021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2082https://doaj.org/toc/2075-4418As urine is not sterile, inflammatory reactions caused by dysbiosis of the urinary microbiota may induce interstitial cystitis. A study was conducted to determine whether β-defensin 2 (BD-2), a specific antimicrobial peptide in the bladder, could be used as a novel diagnostic marker for ulcerative interstitial cystitis (IC). Urine samples from three female groups were examined: healthy controls (<i>n</i> = 34, Control group), non-Hunner type IC (<i>n</i> = 40, NHIC group), and Hunner type IC (<i>n</i> = 68, HIC group). Urine samples were collected via a transurethral catheter and assayed for BD-2 levels using enzyme linked immunosorbent assay. Under general or regional anesthesia, cystoscopy with diagnostic and therapeutic hydrodistension was performed in NHIC and HIC groups patients. These patients underwent a biopsy of the bladders. Based on the urinary specimens from 142 patients, BD-2 expression was found to be 18-fold higher in patients with Hunner type IC than in patients with non-Hunner type IC. The enhanced secretion of BD-2 exhibited a strong correlation with increased mast cell counts associated with bladder IC pathology. Enhanced urinary secretion of the antimicrobial peptide BD-2 from Hunner type IC patients associated with clinical phenotypes and demonstrated relatively robust levels to be used as a potential biomarker. Moreover, the increased urinary level of BD-2 may suggest a new possibility of biomarkers caused by dysbiosis of the urinary microbiota in ulcerative IC.Sang Wook LeeSi Hyun KimKwang Woo LeeWoong Bin KimHae Woong ChoiJi Eun MoonAhrim MoonYoung Ho KimMDPI AGarticleinterstitial cystitisbiomarkersurine specimen collectionantimicrobial peptidebeta-defensinsMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2082, p 2082 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	interstitial cystitis biomarkers urine specimen collection antimicrobial peptide beta-defensins Medicine (General) R5-920
spellingShingle	interstitial cystitis biomarkers urine specimen collection antimicrobial peptide beta-defensins Medicine (General) R5-920 Sang Wook Lee Si Hyun Kim Kwang Woo Lee Woong Bin Kim Hae Woong Choi Ji Eun Moon Ahrim Moon Young Ho Kim β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?
description	As urine is not sterile, inflammatory reactions caused by dysbiosis of the urinary microbiota may induce interstitial cystitis. A study was conducted to determine whether β-defensin 2 (BD-2), a specific antimicrobial peptide in the bladder, could be used as a novel diagnostic marker for ulcerative interstitial cystitis (IC). Urine samples from three female groups were examined: healthy controls (<i>n</i> = 34, Control group), non-Hunner type IC (<i>n</i> = 40, NHIC group), and Hunner type IC (<i>n</i> = 68, HIC group). Urine samples were collected via a transurethral catheter and assayed for BD-2 levels using enzyme linked immunosorbent assay. Under general or regional anesthesia, cystoscopy with diagnostic and therapeutic hydrodistension was performed in NHIC and HIC groups patients. These patients underwent a biopsy of the bladders. Based on the urinary specimens from 142 patients, BD-2 expression was found to be 18-fold higher in patients with Hunner type IC than in patients with non-Hunner type IC. The enhanced secretion of BD-2 exhibited a strong correlation with increased mast cell counts associated with bladder IC pathology. Enhanced urinary secretion of the antimicrobial peptide BD-2 from Hunner type IC patients associated with clinical phenotypes and demonstrated relatively robust levels to be used as a potential biomarker. Moreover, the increased urinary level of BD-2 may suggest a new possibility of biomarkers caused by dysbiosis of the urinary microbiota in ulcerative IC.
format	article
author	Sang Wook Lee Si Hyun Kim Kwang Woo Lee Woong Bin Kim Hae Woong Choi Ji Eun Moon Ahrim Moon Young Ho Kim
author_facet	Sang Wook Lee Si Hyun Kim Kwang Woo Lee Woong Bin Kim Hae Woong Choi Ji Eun Moon Ahrim Moon Young Ho Kim
author_sort	Sang Wook Lee
title	β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?
title_short	β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?
title_full	β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?
title_fullStr	β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?
title_full_unstemmed	β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?
title_sort	β-defensin 2, an antimicrobial peptide, as a novel biomarker for ulcerative interstitial cystitis; can β-defensin 2 suspect the dysbiosis of urine microbiota?
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4d0329e8824344b5a12ba4d36a910c04
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β-Defensin 2, an Antimicrobial Peptide, as a Novel Biomarker for Ulcerative Interstitial Cystitis; Can β-Defensin 2 Suspect the Dysbiosis of Urine Microbiota?

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