Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.

Cluster of differentiation 47 (CD47) is a member of the immunoglobulin superfamily which functions as a ligand for the extracellular region of signal regulatory protein α (SIRPα), a protein which is abundantly expressed in the brain. Previous studies, including ours, have demonstrated that both CD47...

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Autores principales:	Hisatsugu Koshimizu, Keizo Takao, Takashi Matozaki, Hiroshi Ohnishi, Tsuyoshi Miyakawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d0a0df5e86d4017b9f09b0db94e1937
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spelling	oai:doaj.org-article:4d0a0df5e86d4017b9f09b0db94e19372021-11-18T08:31:19ZComprehensive behavioral analysis of cluster of differentiation 47 knockout mice.1932-620310.1371/journal.pone.0089584https://doaj.org/article/4d0a0df5e86d4017b9f09b0db94e19372014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586890/?tool=EBIhttps://doaj.org/toc/1932-6203Cluster of differentiation 47 (CD47) is a member of the immunoglobulin superfamily which functions as a ligand for the extracellular region of signal regulatory protein α (SIRPα), a protein which is abundantly expressed in the brain. Previous studies, including ours, have demonstrated that both CD47 and SIRPα fulfill various functions in the central nervous system (CNS), such as the modulation of synaptic transmission and neuronal cell survival. We previously reported that CD47 is involved in the regulation of depression-like behavior of mice in the forced swim test through its modulation of tyrosine phosphorylation of SIRPα. However, other potential behavioral functions of CD47 remain largely unknown. In this study, in an effort to further investigate functional roles of CD47 in the CNS, CD47 knockout (KO) mice and their wild-type littermates were subjected to a battery of behavioral tests. CD47 KO mice displayed decreased prepulse inhibition, while the startle response did not differ between genotypes. The mutants exhibited slightly but significantly decreased sociability and social novelty preference in Crawley's three-chamber social approach test, whereas in social interaction tests in which experimental and stimulus mice have direct contact with each other in a freely moving setting in a novel environment or home cage, there were no significant differences between the genotypes. While previous studies suggested that CD47 regulates fear memory in the inhibitory avoidance test in rodents, our CD47 KO mice exhibited normal fear and spatial memory in the fear conditioning and the Barnes maze tests, respectively. These findings suggest that CD47 is potentially involved in the regulation of sensorimotor gating and social behavior in mice.Hisatsugu KoshimizuKeizo TakaoTakashi MatozakiHiroshi OhnishiTsuyoshi MiyakawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89584 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hisatsugu Koshimizu Keizo Takao Takashi Matozaki Hiroshi Ohnishi Tsuyoshi Miyakawa Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
description	Cluster of differentiation 47 (CD47) is a member of the immunoglobulin superfamily which functions as a ligand for the extracellular region of signal regulatory protein α (SIRPα), a protein which is abundantly expressed in the brain. Previous studies, including ours, have demonstrated that both CD47 and SIRPα fulfill various functions in the central nervous system (CNS), such as the modulation of synaptic transmission and neuronal cell survival. We previously reported that CD47 is involved in the regulation of depression-like behavior of mice in the forced swim test through its modulation of tyrosine phosphorylation of SIRPα. However, other potential behavioral functions of CD47 remain largely unknown. In this study, in an effort to further investigate functional roles of CD47 in the CNS, CD47 knockout (KO) mice and their wild-type littermates were subjected to a battery of behavioral tests. CD47 KO mice displayed decreased prepulse inhibition, while the startle response did not differ between genotypes. The mutants exhibited slightly but significantly decreased sociability and social novelty preference in Crawley's three-chamber social approach test, whereas in social interaction tests in which experimental and stimulus mice have direct contact with each other in a freely moving setting in a novel environment or home cage, there were no significant differences between the genotypes. While previous studies suggested that CD47 regulates fear memory in the inhibitory avoidance test in rodents, our CD47 KO mice exhibited normal fear and spatial memory in the fear conditioning and the Barnes maze tests, respectively. These findings suggest that CD47 is potentially involved in the regulation of sensorimotor gating and social behavior in mice.
format	article
author	Hisatsugu Koshimizu Keizo Takao Takashi Matozaki Hiroshi Ohnishi Tsuyoshi Miyakawa
author_facet	Hisatsugu Koshimizu Keizo Takao Takashi Matozaki Hiroshi Ohnishi Tsuyoshi Miyakawa
author_sort	Hisatsugu Koshimizu
title	Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
title_short	Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
title_full	Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
title_fullStr	Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
title_full_unstemmed	Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
title_sort	comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4d0a0df5e86d4017b9f09b0db94e1937
work_keys_str_mv	AT hisatsugukoshimizu comprehensivebehavioralanalysisofclusterofdifferentiation47knockoutmice AT keizotakao comprehensivebehavioralanalysisofclusterofdifferentiation47knockoutmice AT takashimatozaki comprehensivebehavioralanalysisofclusterofdifferentiation47knockoutmice AT hiroshiohnishi comprehensivebehavioralanalysisofclusterofdifferentiation47knockoutmice AT tsuyoshimiyakawa comprehensivebehavioralanalysisofclusterofdifferentiation47knockoutmice
_version_	1718421681247092736

Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.

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