Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells

BACKGROUND AND OBJECTIVE: Application of stem cells in medical research and treatment has made a new horizon to progress in many disorders. So finding methods for better isolation and culture and maintenance of stem cells (SC) has a specific importance. Aim of this research was to evaluate the prote...

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Autores principales:	AR Shams, N Forghani, F Aghaee, P Yagmaee, AR Rahimian
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2009
Materias:	embryonic stem cells feeder layer alkaline phosphates mesenchymal stem cells mouse embryonic fibroblast 3t3 Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4d0d9d2510eb441c9cf205f8ee45c72e
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spelling	oai:doaj.org-article:4d0d9d2510eb441c9cf205f8ee45c72e2021-11-10T09:03:07ZEvaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells1561-41072251-7170https://doaj.org/article/4d0d9d2510eb441c9cf205f8ee45c72e2009-12-01T00:00:00Zhttp://jbums.org/article-1-3355-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Application of stem cells in medical research and treatment has made a new horizon to progress in many disorders. So finding methods for better isolation and culture and maintenance of stem cells (SC) has a specific importance. Aim of this research was to evaluate the protective effects of 3 different layers of mesenchymal stem cells (MSC), mouse embryonic fibroblast (MEF) and 3T3 on Sc for increasing the amounts of cells for transplantation and differentiation. METHODS: Blastocysts were obtained from 10 Balb/c pregnant mice. Collected embryos were put on 3 different feeder layers of mouse embryonic fibroblast (MEFs), Mesenchymal stem cells (MSC) and 3T3 cell line. Two or 3 days later, the zona pellucida was removed and after 5 days the inner cell mass (ICM) growth, was removed mechanically and changed to small multi-cellular clumps through trypsination. Then formed colonies were cultured on feeder layers. Finally the colonies were identified through staining with alkaline phosphates for their numbers and morphological characteristics.FINDINGS: From 29 embryos put on MEF layer all t of them (100%) was attached. From 32 embryos put on the MSC layer (64.5%) was attached. While the amount of attaching of 20 blastocysts to the 3T3 feeder layer was low and none of them reach to the growth stage of the inner cell mass. The amount of colonies formation during three passages on MEF feeder layer was more than MSC feeder layer. Data analysis demonstrated a significant difference (p<0.05) in percentage and time of hatching and time for living of blastocysts CONCLUSION: Considering these three feeder layers, MEF was better than MSC and both of them had priority to 3T3.AR Shams,N Forghani,F Aghaee,P YagmaeeAR RahimianBabol University of Medical Sciencesarticleembryonic stem cellsfeeder layeralkaline phosphatesmesenchymal stem cellsmouse embryonic fibroblast3t3MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 11, Iss 5, Pp 7-15 (2009)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	embryonic stem cells feeder layer alkaline phosphates mesenchymal stem cells mouse embryonic fibroblast 3t3 Medicine R Medicine (General) R5-920
spellingShingle	embryonic stem cells feeder layer alkaline phosphates mesenchymal stem cells mouse embryonic fibroblast 3t3 Medicine R Medicine (General) R5-920 AR Shams, N Forghani, F Aghaee, P Yagmaee AR Rahimian Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells
description	BACKGROUND AND OBJECTIVE: Application of stem cells in medical research and treatment has made a new horizon to progress in many disorders. So finding methods for better isolation and culture and maintenance of stem cells (SC) has a specific importance. Aim of this research was to evaluate the protective effects of 3 different layers of mesenchymal stem cells (MSC), mouse embryonic fibroblast (MEF) and 3T3 on Sc for increasing the amounts of cells for transplantation and differentiation. METHODS: Blastocysts were obtained from 10 Balb/c pregnant mice. Collected embryos were put on 3 different feeder layers of mouse embryonic fibroblast (MEFs), Mesenchymal stem cells (MSC) and 3T3 cell line. Two or 3 days later, the zona pellucida was removed and after 5 days the inner cell mass (ICM) growth, was removed mechanically and changed to small multi-cellular clumps through trypsination. Then formed colonies were cultured on feeder layers. Finally the colonies were identified through staining with alkaline phosphates for their numbers and morphological characteristics.FINDINGS: From 29 embryos put on MEF layer all t of them (100%) was attached. From 32 embryos put on the MSC layer (64.5%) was attached. While the amount of attaching of 20 blastocysts to the 3T3 feeder layer was low and none of them reach to the growth stage of the inner cell mass. The amount of colonies formation during three passages on MEF feeder layer was more than MSC feeder layer. Data analysis demonstrated a significant difference (p<0.05) in percentage and time of hatching and time for living of blastocysts CONCLUSION: Considering these three feeder layers, MEF was better than MSC and both of them had priority to 3T3.
format	article
author	AR Shams, N Forghani, F Aghaee, P Yagmaee AR Rahimian
author_facet	AR Shams, N Forghani, F Aghaee, P Yagmaee AR Rahimian
author_sort	AR Shams,
title	Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells
title_short	Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells
title_full	Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells
title_fullStr	Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells
title_full_unstemmed	Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells
title_sort	evaluation of protective effects of different feeder layers on mouse embryonic stem cells
publisher	Babol University of Medical Sciences
publishDate	2009
url	https://doaj.org/article/4d0d9d2510eb441c9cf205f8ee45c72e
work_keys_str_mv	AT arshams evaluationofprotectiveeffectsofdifferentfeederlayersonmouseembryonicstemcells AT nforghani evaluationofprotectiveeffectsofdifferentfeederlayersonmouseembryonicstemcells AT faghaee evaluationofprotectiveeffectsofdifferentfeederlayersonmouseembryonicstemcells AT pyagmaee evaluationofprotectiveeffectsofdifferentfeederlayersonmouseembryonicstemcells AT arrahimian evaluationofprotectiveeffectsofdifferentfeederlayersonmouseembryonicstemcells
_version_	1718440278870720512

Evaluation of Protective Effects of Different Feeder Layers on Mouse Embryonic Stem Cells

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