Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:4d0fe2ef9a604f0fb6865d1e08b1ed50 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:4d0fe2ef9a604f0fb6865d1e08b1ed502021-12-02T13:31:14ZExtracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping10.1038/s41467-020-14957-y2041-1723https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed502020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14957-yhttps://doaj.org/toc/2041-1723Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.Peter GeeMandy S. Y. LungYuya OkuzakiNoriko SasakawaTakahiro IguchiYukimasa MakitaHiroyuki HozumiYasutomo MiuraLucy F. YangMio IwasakiXiou H. WangMatthew A. WallerNanako ShiraiYasuko O. AbeYoko FujitaKei WatanabeAkihiro KagitaKumiko A. IwabuchiMasahiko YasudaHuaigeng XuTakeshi NodaJun KomanoHidetoshi SakuraiNaoto InukaiAkitsu HottaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-18 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Peter Gee Mandy S. Y. Lung Yuya Okuzaki Noriko Sasakawa Takahiro Iguchi Yukimasa Makita Hiroyuki Hozumi Yasutomo Miura Lucy F. Yang Mio Iwasaki Xiou H. Wang Matthew A. Waller Nanako Shirai Yasuko O. Abe Yoko Fujita Kei Watanabe Akihiro Kagita Kumiko A. Iwabuchi Masahiko Yasuda Huaigeng Xu Takeshi Noda Jun Komano Hidetoshi Sakurai Naoto Inukai Akitsu Hotta Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping |
| description |
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells. |
| format |
article |
| author |
Peter Gee Mandy S. Y. Lung Yuya Okuzaki Noriko Sasakawa Takahiro Iguchi Yukimasa Makita Hiroyuki Hozumi Yasutomo Miura Lucy F. Yang Mio Iwasaki Xiou H. Wang Matthew A. Waller Nanako Shirai Yasuko O. Abe Yoko Fujita Kei Watanabe Akihiro Kagita Kumiko A. Iwabuchi Masahiko Yasuda Huaigeng Xu Takeshi Noda Jun Komano Hidetoshi Sakurai Naoto Inukai Akitsu Hotta |
| author_facet |
Peter Gee Mandy S. Y. Lung Yuya Okuzaki Noriko Sasakawa Takahiro Iguchi Yukimasa Makita Hiroyuki Hozumi Yasutomo Miura Lucy F. Yang Mio Iwasaki Xiou H. Wang Matthew A. Waller Nanako Shirai Yasuko O. Abe Yoko Fujita Kei Watanabe Akihiro Kagita Kumiko A. Iwabuchi Masahiko Yasuda Huaigeng Xu Takeshi Noda Jun Komano Hidetoshi Sakurai Naoto Inukai Akitsu Hotta |
| author_sort |
Peter Gee |
| title |
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping |
| title_short |
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping |
| title_full |
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping |
| title_fullStr |
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping |
| title_full_unstemmed |
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping |
| title_sort |
extracellular nanovesicles for packaging of crispr-cas9 protein and sgrna to induce therapeutic exon skipping |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50 |
| work_keys_str_mv |
AT petergee extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT mandysylung extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT yuyaokuzaki extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT norikosasakawa extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT takahiroiguchi extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT yukimasamakita extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT hiroyukihozumi extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT yasutomomiura extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT lucyfyang extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT mioiwasaki extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT xiouhwang extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT matthewawaller extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT nanakoshirai extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT yasukooabe extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT yokofujita extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT keiwatanabe extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT akihirokagita extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT kumikoaiwabuchi extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT masahikoyasuda extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT huaigengxu extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT takeshinoda extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT junkomano extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT hidetoshisakurai extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT naotoinukai extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping AT akitsuhotta extracellularnanovesiclesforpackagingofcrisprcas9proteinandsgrnatoinducetherapeuticexonskipping |
| _version_ |
1718392850673041408 |