A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves

Abstract The aim was to examine the effect of rapid body weight gain during early calfhood consistent with earlier sexual development on the transcriptional profile of the hypothalamus. Angus X Holstein–Friesian heifer calves (19 ± 5 days of age) were offered a high (HI, n = 14) or moderate (MOD, n ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	José M. Sánchez, Kate Keogh, Alan K. Kelly, Colin J. Byrne, Pat Lonergan, David A. Kenny
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d10c9900a474594a667aab4e9ce93b9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d10c9900a474594a667aab4e9ce93b9
record_format	dspace
spelling	oai:doaj.org-article:4d10c9900a474594a667aab4e9ce93b92021-12-02T15:23:17ZA high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves10.1038/s41598-021-93080-42045-2322https://doaj.org/article/4d10c9900a474594a667aab4e9ce93b92021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93080-4https://doaj.org/toc/2045-2322Abstract The aim was to examine the effect of rapid body weight gain during early calfhood consistent with earlier sexual development on the transcriptional profile of the hypothalamus. Angus X Holstein–Friesian heifer calves (19 ± 5 days of age) were offered a high (HI, n = 14) or moderate (MOD, n = 15) plane of nutrition from 3 to 21 weeks of age to achieve a growth rate of 1.2 kg/d and 0.5 kg/d, respectively. Following euthanasia at 21 weeks, the arcuate nucleus (ARC) region was separated from the remainder of the hypothalamus and both were subjected to RNA-Seq. HI calves exhibited altered expression of 80 and 39 transcripts in the ARC and the remaining hypothalamus, respectively (P < 0.05) including downregulation of AGRP and NPY and upregulation of POMC, previously implicated in precocious sexual development. Stress-signaling pathways were amongst the most highly dysregulated. Organ morphology, reproductive system development and function, and developmental disorder were amongst the networks derived from differentially expressed genes (DEGs) in the ARC. Gene co-expression analysis revealed DEGs within the ARC (POMC, CBLN2, CHGA) and hypothalamus (PENK) as hub genes. In conclusion, enhanced nutrition during early calfhood alters the biochemical regulation of the hypothalamus consistent with advanced sexual development in the prepubertal heifer.José M. SánchezKate KeoghAlan K. KellyColin J. ByrnePat LonerganDavid A. KennyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q José M. Sánchez Kate Keogh Alan K. Kelly Colin J. Byrne Pat Lonergan David A. Kenny A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
description	Abstract The aim was to examine the effect of rapid body weight gain during early calfhood consistent with earlier sexual development on the transcriptional profile of the hypothalamus. Angus X Holstein–Friesian heifer calves (19 ± 5 days of age) were offered a high (HI, n = 14) or moderate (MOD, n = 15) plane of nutrition from 3 to 21 weeks of age to achieve a growth rate of 1.2 kg/d and 0.5 kg/d, respectively. Following euthanasia at 21 weeks, the arcuate nucleus (ARC) region was separated from the remainder of the hypothalamus and both were subjected to RNA-Seq. HI calves exhibited altered expression of 80 and 39 transcripts in the ARC and the remaining hypothalamus, respectively (P < 0.05) including downregulation of AGRP and NPY and upregulation of POMC, previously implicated in precocious sexual development. Stress-signaling pathways were amongst the most highly dysregulated. Organ morphology, reproductive system development and function, and developmental disorder were amongst the networks derived from differentially expressed genes (DEGs) in the ARC. Gene co-expression analysis revealed DEGs within the ARC (POMC, CBLN2, CHGA) and hypothalamus (PENK) as hub genes. In conclusion, enhanced nutrition during early calfhood alters the biochemical regulation of the hypothalamus consistent with advanced sexual development in the prepubertal heifer.
format	article
author	José M. Sánchez Kate Keogh Alan K. Kelly Colin J. Byrne Pat Lonergan David A. Kenny
author_facet	José M. Sánchez Kate Keogh Alan K. Kelly Colin J. Byrne Pat Lonergan David A. Kenny
author_sort	José M. Sánchez
title	A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
title_short	A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
title_full	A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
title_fullStr	A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
title_full_unstemmed	A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
title_sort	high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4d10c9900a474594a667aab4e9ce93b9
work_keys_str_mv	AT josemsanchez ahighplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT katekeogh ahighplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT alankkelly ahighplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT colinjbyrne ahighplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT patlonergan ahighplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT davidakenny ahighplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT josemsanchez highplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT katekeogh highplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT alankkelly highplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT colinjbyrne highplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT patlonergan highplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves AT davidakenny highplaneofnutritionduringearlylifealtersthehypothalamictranscriptomeofheifercalves
_version_	1718387263079972864

A high plane of nutrition during early life alters the hypothalamic transcriptome of heifer calves

Ejemplares similares