Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

Abstract The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christof Hepp, Nicolas Shiaelis, Nicole C. Robb, Alison Vaughan, Philippa C. Matthews, Nicole Stoesser, Derrick Crook, Achillefs N. Kapanidis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d11296b02a54aa2b9477fd8652b9037
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d11296b02a54aa2b9477fd8652b9037
record_format	dspace
spelling	oai:doaj.org-article:4d11296b02a54aa2b9477fd8652b90372021-12-02T19:17:05ZViral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA10.1038/s41598-021-98972-z2045-2322https://doaj.org/article/4d11296b02a54aa2b9477fd8652b90372021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98972-zhttps://doaj.org/toc/2045-2322Abstract The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.Christof HeppNicolas ShiaelisNicole C. RobbAlison VaughanPhilippa C. MatthewsNicole StoesserDerrick CrookAchillefs N. KapanidisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christof Hepp Nicolas Shiaelis Nicole C. Robb Alison Vaughan Philippa C. Matthews Nicole Stoesser Derrick Crook Achillefs N. Kapanidis Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
description	Abstract The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
format	article
author	Christof Hepp Nicolas Shiaelis Nicole C. Robb Alison Vaughan Philippa C. Matthews Nicole Stoesser Derrick Crook Achillefs N. Kapanidis
author_facet	Christof Hepp Nicolas Shiaelis Nicole C. Robb Alison Vaughan Philippa C. Matthews Nicole Stoesser Derrick Crook Achillefs N. Kapanidis
author_sort	Christof Hepp
title	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_short	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_full	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_fullStr	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_full_unstemmed	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_sort	viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral rna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4d11296b02a54aa2b9477fd8652b9037
work_keys_str_mv	AT christofhepp viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT nicolasshiaelis viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT nicolecrobb viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT alisonvaughan viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT philippacmatthews viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT nicolestoesser viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT derrickcrook viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna AT achillefsnkapanidis viraldetectionandidentificationin20minbyrapidsingleparticlefluorescenceinsituhybridizationofviralrna
_version_	1718376910792163328

Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

Ejemplares similares