Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.
<h4>Background</h4>Abdominal aortic aneurysm (AAA) is characterized by increased aortic vessel wall diameter (>1.5 times normal) and loss of parallelism. This disease is responsible for 1-4% mortality occurring on rupture in males older than 65 years. Due to its asymptomatic nature, p...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/4d1418f36cc942e09c797782b8dc60c3 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:4d1418f36cc942e09c797782b8dc60c3 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:4d1418f36cc942e09c797782b8dc60c32021-11-18T07:32:48ZQuantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.1932-620310.1371/journal.pone.0028698https://doaj.org/article/4d1418f36cc942e09c797782b8dc60c32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22163325/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Abdominal aortic aneurysm (AAA) is characterized by increased aortic vessel wall diameter (>1.5 times normal) and loss of parallelism. This disease is responsible for 1-4% mortality occurring on rupture in males older than 65 years. Due to its asymptomatic nature, proteomic techniques were used to search for diagnostic biomarkers that might allow surgical intervention under nonlife threatening conditions.<h4>Methodology/principal findings</h4>Pooled human plasma samples of 17 AAA and 17 control patients were depleted of the most abundant proteins and compared using a data-independent shotgun proteomic strategy, Precursor Acquisition Independent From Ion Count (PAcIFIC), combined with spectral counting and isobaric tandem mass tags. Both quantitative methods collectively identified 80 proteins as statistically differentially abundant between AAA and control patients. Among differentially abundant proteins, a subgroup of 19 was selected according to Gene Ontology classification and implication in AAA for verification by Western blot (WB) in the same 34 individual plasma samples that comprised the pools. From the 19 proteins, 12 were detected by WB. Five of them were verified to be differentially up-regulated in individual plasma of AAA patients: adiponectin, extracellular superoxide dismutase, protein AMBP, kallistatin and carboxypeptidase B2.<h4>Conclusions/significance</h4>Plasma depletion of high abundance proteins combined with quantitative PAcIFIC analysis offered an efficient and sensitive tool for the screening of new potential biomarkers of AAA. However, WB analysis to verify the 19 PAcIFIC identified proteins of interest proved inconclusive save for five proteins. We discuss these five in terms of their potential relevance as biological markers for use in AAA screening of population at risk.Adelina E Acosta-MartinAlexandre PanchaudMaggy ChwastyniakAnnabelle DupontFrancis JuthierCorinne GautierBrigitte JudePhilippe AmouyelDavid R GoodlettFlorence PinetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28698 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Adelina E Acosta-Martin Alexandre Panchaud Maggy Chwastyniak Annabelle Dupont Francis Juthier Corinne Gautier Brigitte Jude Philippe Amouyel David R Goodlett Florence Pinet Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| description |
<h4>Background</h4>Abdominal aortic aneurysm (AAA) is characterized by increased aortic vessel wall diameter (>1.5 times normal) and loss of parallelism. This disease is responsible for 1-4% mortality occurring on rupture in males older than 65 years. Due to its asymptomatic nature, proteomic techniques were used to search for diagnostic biomarkers that might allow surgical intervention under nonlife threatening conditions.<h4>Methodology/principal findings</h4>Pooled human plasma samples of 17 AAA and 17 control patients were depleted of the most abundant proteins and compared using a data-independent shotgun proteomic strategy, Precursor Acquisition Independent From Ion Count (PAcIFIC), combined with spectral counting and isobaric tandem mass tags. Both quantitative methods collectively identified 80 proteins as statistically differentially abundant between AAA and control patients. Among differentially abundant proteins, a subgroup of 19 was selected according to Gene Ontology classification and implication in AAA for verification by Western blot (WB) in the same 34 individual plasma samples that comprised the pools. From the 19 proteins, 12 were detected by WB. Five of them were verified to be differentially up-regulated in individual plasma of AAA patients: adiponectin, extracellular superoxide dismutase, protein AMBP, kallistatin and carboxypeptidase B2.<h4>Conclusions/significance</h4>Plasma depletion of high abundance proteins combined with quantitative PAcIFIC analysis offered an efficient and sensitive tool for the screening of new potential biomarkers of AAA. However, WB analysis to verify the 19 PAcIFIC identified proteins of interest proved inconclusive save for five proteins. We discuss these five in terms of their potential relevance as biological markers for use in AAA screening of population at risk. |
| format |
article |
| author |
Adelina E Acosta-Martin Alexandre Panchaud Maggy Chwastyniak Annabelle Dupont Francis Juthier Corinne Gautier Brigitte Jude Philippe Amouyel David R Goodlett Florence Pinet |
| author_facet |
Adelina E Acosta-Martin Alexandre Panchaud Maggy Chwastyniak Annabelle Dupont Francis Juthier Corinne Gautier Brigitte Jude Philippe Amouyel David R Goodlett Florence Pinet |
| author_sort |
Adelina E Acosta-Martin |
| title |
Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| title_short |
Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| title_full |
Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| title_fullStr |
Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| title_full_unstemmed |
Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| title_sort |
quantitative mass spectrometry analysis using pacific for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/4d1418f36cc942e09c797782b8dc60c3 |
| work_keys_str_mv |
AT adelinaeacostamartin quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT alexandrepanchaud quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT maggychwastyniak quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT annabelledupont quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT francisjuthier quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT corinnegautier quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT brigittejude quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT philippeamouyel quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT davidrgoodlett quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm AT florencepinet quantitativemassspectrometryanalysisusingpacificfortheidentificationofplasmadiagnosticbiomarkersforabdominalaorticaneurysm |
| _version_ |
1718423282186715136 |