Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells.
The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa...
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oai:doaj.org-article:4d1cb57c12dc44a59bddbdd730c55e432021-11-18T07:45:12ZEngineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells.1932-620310.1371/journal.pone.0063129https://doaj.org/article/4d1cb57c12dc44a59bddbdd730c55e432013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23700414/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative ΔpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems.Victor Bochuan WangSong-Lin ChuaBin CaoThomas SeviourVictor J NesatyyEnrico MarsiliStaffan KjellebergMichael GivskovTim Tolker-NielsenHao SongJoachim Say Chye LooLiang YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63129 (2013) |
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Medicine R Science Q |
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Medicine R Science Q Victor Bochuan Wang Song-Lin Chua Bin Cao Thomas Seviour Victor J Nesatyy Enrico Marsili Staffan Kjelleberg Michael Givskov Tim Tolker-Nielsen Hao Song Joachim Say Chye Loo Liang Yang Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| description |
The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative ΔpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems. |
| format |
article |
| author |
Victor Bochuan Wang Song-Lin Chua Bin Cao Thomas Seviour Victor J Nesatyy Enrico Marsili Staffan Kjelleberg Michael Givskov Tim Tolker-Nielsen Hao Song Joachim Say Chye Loo Liang Yang |
| author_facet |
Victor Bochuan Wang Song-Lin Chua Bin Cao Thomas Seviour Victor J Nesatyy Enrico Marsili Staffan Kjelleberg Michael Givskov Tim Tolker-Nielsen Hao Song Joachim Say Chye Loo Liang Yang |
| author_sort |
Victor Bochuan Wang |
| title |
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| title_short |
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| title_full |
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| title_fullStr |
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| title_full_unstemmed |
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| title_sort |
engineering pqs biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/4d1cb57c12dc44a59bddbdd730c55e43 |
| work_keys_str_mv |
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