Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).

A growing body of work on the neuroethology of cubozoans is based largely on the capabilities of the photoreceptive tissues, and it is important to determine the molecular basis of their light sensitivity. The cubozoans rely on 24 special purpose eyes to extract specific information from a complex v...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jan Bielecki, Alexander K Zaharoff, Nicole Y Leung, Anders Garm, Todd H Oakley
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d203c9657d44e0fa9f8d055e8588273
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d203c9657d44e0fa9f8d055e8588273
record_format	dspace
spelling	oai:doaj.org-article:4d203c9657d44e0fa9f8d055e85882732021-11-18T08:17:01ZOcular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).1932-620310.1371/journal.pone.0098870https://doaj.org/article/4d203c9657d44e0fa9f8d055e85882732014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24901369/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A growing body of work on the neuroethology of cubozoans is based largely on the capabilities of the photoreceptive tissues, and it is important to determine the molecular basis of their light sensitivity. The cubozoans rely on 24 special purpose eyes to extract specific information from a complex visual scene to guide their behavior in the habitat. The lens eyes are the most studied photoreceptive structures, and the phototransduction in the photoreceptor cells is based on light sensitive opsin molecules. Opsins are photosensitive transmembrane proteins associated with photoreceptors in eyes, and the amino acid sequence of the opsins determines the spectral properties of the photoreceptors. Here we show that two distinct opsins (Tripedalia cystophora-lens eye expressed opsin and Tripedalia cystophora-neuropil expressed opsin, or Tc-leo and Tc-neo) are expressed in the Tripedalia cystophora rhopalium. Quantitative PCR determined the level of expression of the two opsins, and we found Tc-leo to have a higher amount of expression than Tc-neo. In situ hybridization located Tc-leo expression in the retinal photoreceptors of the lens eyes where the opsin is involved in image formation. Tc-neo is expressed in a confined part of the neuropil and is probably involved in extraocular light sensation, presumably in relation to diurnal activity.Jan BieleckiAlexander K ZaharoffNicole Y LeungAnders GarmTodd H OakleyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98870 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jan Bielecki Alexander K Zaharoff Nicole Y Leung Anders Garm Todd H Oakley Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).
description	A growing body of work on the neuroethology of cubozoans is based largely on the capabilities of the photoreceptive tissues, and it is important to determine the molecular basis of their light sensitivity. The cubozoans rely on 24 special purpose eyes to extract specific information from a complex visual scene to guide their behavior in the habitat. The lens eyes are the most studied photoreceptive structures, and the phototransduction in the photoreceptor cells is based on light sensitive opsin molecules. Opsins are photosensitive transmembrane proteins associated with photoreceptors in eyes, and the amino acid sequence of the opsins determines the spectral properties of the photoreceptors. Here we show that two distinct opsins (Tripedalia cystophora-lens eye expressed opsin and Tripedalia cystophora-neuropil expressed opsin, or Tc-leo and Tc-neo) are expressed in the Tripedalia cystophora rhopalium. Quantitative PCR determined the level of expression of the two opsins, and we found Tc-leo to have a higher amount of expression than Tc-neo. In situ hybridization located Tc-leo expression in the retinal photoreceptors of the lens eyes where the opsin is involved in image formation. Tc-neo is expressed in a confined part of the neuropil and is probably involved in extraocular light sensation, presumably in relation to diurnal activity.
format	article
author	Jan Bielecki Alexander K Zaharoff Nicole Y Leung Anders Garm Todd H Oakley
author_facet	Jan Bielecki Alexander K Zaharoff Nicole Y Leung Anders Garm Todd H Oakley
author_sort	Jan Bielecki
title	Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).
title_short	Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).
title_full	Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).
title_fullStr	Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).
title_full_unstemmed	Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).
title_sort	ocular and extraocular expression of opsins in the rhopalium of tripedalia cystophora (cnidaria: cubozoa).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4d203c9657d44e0fa9f8d055e8588273
work_keys_str_mv	AT janbielecki ocularandextraocularexpressionofopsinsintherhopaliumoftripedaliacystophoracnidariacubozoa AT alexanderkzaharoff ocularandextraocularexpressionofopsinsintherhopaliumoftripedaliacystophoracnidariacubozoa AT nicoleyleung ocularandextraocularexpressionofopsinsintherhopaliumoftripedaliacystophoracnidariacubozoa AT andersgarm ocularandextraocularexpressionofopsinsintherhopaliumoftripedaliacystophoracnidariacubozoa AT toddhoakley ocularandextraocularexpressionofopsinsintherhopaliumoftripedaliacystophoracnidariacubozoa
_version_	1718421982442160128

Ocular and extraocular expression of opsins in the rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa).

Ejemplares similares