The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms

Summary: A rigid cell wall defines the morphology of most bacteria. MreB, a bacterial homologue of actin, plays a major role in coordinating cell wall biogenesis and defining cell shape. Spiroplasma are wall-less bacteria that robustly grow with a characteristic helical shape. Paradoxal to their lac...

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Autores principales:	Florent Masson, Xavier Pierrat, Bruno Lemaitre, Alexandre Persat
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biological sciences Molecular biology Cell biology Biomechanics Science Q
Acceso en línea:	https://doaj.org/article/4d2915adf5ef41c0b8ac6133555040e3
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spelling	oai:doaj.org-article:4d2915adf5ef41c0b8ac6133555040e32021-11-28T04:36:49ZThe wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms2589-004210.1016/j.isci.2021.103458https://doaj.org/article/4d2915adf5ef41c0b8ac6133555040e32021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221014292https://doaj.org/toc/2589-0042Summary: A rigid cell wall defines the morphology of most bacteria. MreB, a bacterial homologue of actin, plays a major role in coordinating cell wall biogenesis and defining cell shape. Spiroplasma are wall-less bacteria that robustly grow with a characteristic helical shape. Paradoxal to their lack of cell wall, the Spiroplasma genome contains five homologs of MreB (SpMreBs). Here, we investigate the function of SpMreBs in forming a polymeric cytoskeleton. We found that, in vivo, Spiroplasma maintain a high concentration of all MreB isoforms. By leveraging a heterologous expression system that bypasses the poor genetic tractability of Spiroplasma, we found that SpMreBs produced polymeric filaments of various morphologies. We characterized an interaction network between isoforms that regulate filament formation and patterning. Therefore, our results support the hypothesis where combined SpMreB isoforms would form an inner polymeric cytoskeleton in vivo that shapes the cell in a wall-independent manner.Florent MassonXavier PierratBruno LemaitreAlexandre PersatElsevierarticleBiological sciencesMolecular biologyCell biologyBiomechanicsScienceQENiScience, Vol 24, Iss 12, Pp 103458- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biological sciences Molecular biology Cell biology Biomechanics Science Q
spellingShingle	Biological sciences Molecular biology Cell biology Biomechanics Science Q Florent Masson Xavier Pierrat Bruno Lemaitre Alexandre Persat The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
description	Summary: A rigid cell wall defines the morphology of most bacteria. MreB, a bacterial homologue of actin, plays a major role in coordinating cell wall biogenesis and defining cell shape. Spiroplasma are wall-less bacteria that robustly grow with a characteristic helical shape. Paradoxal to their lack of cell wall, the Spiroplasma genome contains five homologs of MreB (SpMreBs). Here, we investigate the function of SpMreBs in forming a polymeric cytoskeleton. We found that, in vivo, Spiroplasma maintain a high concentration of all MreB isoforms. By leveraging a heterologous expression system that bypasses the poor genetic tractability of Spiroplasma, we found that SpMreBs produced polymeric filaments of various morphologies. We characterized an interaction network between isoforms that regulate filament formation and patterning. Therefore, our results support the hypothesis where combined SpMreB isoforms would form an inner polymeric cytoskeleton in vivo that shapes the cell in a wall-independent manner.
format	article
author	Florent Masson Xavier Pierrat Bruno Lemaitre Alexandre Persat
author_facet	Florent Masson Xavier Pierrat Bruno Lemaitre Alexandre Persat
author_sort	Florent Masson
title	The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
title_short	The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
title_full	The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
title_fullStr	The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
title_full_unstemmed	The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
title_sort	wall-less bacterium spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting mreb isoforms
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4d2915adf5ef41c0b8ac6133555040e3
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The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms

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