Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure

Abstract Bioartificial livers (BAL) may offer acute liver failure (ALF) patients an opportunity for cure without liver transplantation. We evaluated the efficacy of a spheroid-based BAL system, containing aggregates of porcine hepatocytes, in a porcine model of ALF. ALF pigs were divided into three...

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Autores principales:	Ji-Hyun Lee, Doo-Hoon Lee, Sanghoon Lee, Choon Hyuck David Kwon, Jae-Nam Ryu, Jeong-Kwon Noh, In Keun Jang, Hey-Jung Park, Hee-Hoon Yoon, Jung-Keug Park, Young-Jin Kim, Sung-Koo Kim, Suk-Koo Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d3d098741bc4c8d87a80ac7af71fc95
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spelling	oai:doaj.org-article:4d3d098741bc4c8d87a80ac7af71fc952021-12-02T11:40:20ZFunctional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure10.1038/s41598-017-03424-22045-2322https://doaj.org/article/4d3d098741bc4c8d87a80ac7af71fc952017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03424-2https://doaj.org/toc/2045-2322Abstract Bioartificial livers (BAL) may offer acute liver failure (ALF) patients an opportunity for cure without liver transplantation. We evaluated the efficacy of a spheroid-based BAL system, containing aggregates of porcine hepatocytes, in a porcine model of ALF. ALF pigs were divided into three groups. The control group consisted of treatment naïve pigs (n = 5), blank group consisted of pigs that were attached to the BAL system not containing hepatocytes for 12 hours (n = 5) and BAL group consisted of pigs that were attached to the BAL containing hepatocytes for 12 hours (n = 5). Increase in serum ammonia levels were significantly greater in the blank group (P < 0.01) and control group (P < 0.01), compared to the BAL group during the treatment period. Increase in ICP was significantly greater in the control group compared to the BAL group (P = 0.01). Survival was significantly prolonged in the BAL group compared to the blank group (P = 0.03). A BAL system with a bioreactor containing hepatocyte spheroids showed effective clearance of serum ammonia, preservation of renal function and delayed ICP increase in a porcine model of ALF.Ji-Hyun LeeDoo-Hoon LeeSanghoon LeeChoon Hyuck David KwonJae-Nam RyuJeong-Kwon NohIn Keun JangHey-Jung ParkHee-Hoon YoonJung-Keug ParkYoung-Jin KimSung-Koo KimSuk-Koo LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji-Hyun Lee Doo-Hoon Lee Sanghoon Lee Choon Hyuck David Kwon Jae-Nam Ryu Jeong-Kwon Noh In Keun Jang Hey-Jung Park Hee-Hoon Yoon Jung-Keug Park Young-Jin Kim Sung-Koo Kim Suk-Koo Lee Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
description	Abstract Bioartificial livers (BAL) may offer acute liver failure (ALF) patients an opportunity for cure without liver transplantation. We evaluated the efficacy of a spheroid-based BAL system, containing aggregates of porcine hepatocytes, in a porcine model of ALF. ALF pigs were divided into three groups. The control group consisted of treatment naïve pigs (n = 5), blank group consisted of pigs that were attached to the BAL system not containing hepatocytes for 12 hours (n = 5) and BAL group consisted of pigs that were attached to the BAL containing hepatocytes for 12 hours (n = 5). Increase in serum ammonia levels were significantly greater in the blank group (P < 0.01) and control group (P < 0.01), compared to the BAL group during the treatment period. Increase in ICP was significantly greater in the control group compared to the BAL group (P = 0.01). Survival was significantly prolonged in the BAL group compared to the blank group (P = 0.03). A BAL system with a bioreactor containing hepatocyte spheroids showed effective clearance of serum ammonia, preservation of renal function and delayed ICP increase in a porcine model of ALF.
format	article
author	Ji-Hyun Lee Doo-Hoon Lee Sanghoon Lee Choon Hyuck David Kwon Jae-Nam Ryu Jeong-Kwon Noh In Keun Jang Hey-Jung Park Hee-Hoon Yoon Jung-Keug Park Young-Jin Kim Sung-Koo Kim Suk-Koo Lee
author_facet	Ji-Hyun Lee Doo-Hoon Lee Sanghoon Lee Choon Hyuck David Kwon Jae-Nam Ryu Jeong-Kwon Noh In Keun Jang Hey-Jung Park Hee-Hoon Yoon Jung-Keug Park Young-Jin Kim Sung-Koo Kim Suk-Koo Lee
author_sort	Ji-Hyun Lee
title	Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
title_short	Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
title_full	Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
title_fullStr	Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
title_full_unstemmed	Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
title_sort	functional evaluation of a bioartificial liver support system using immobilized hepatocyte spheroids in a porcine model of acute liver failure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4d3d098741bc4c8d87a80ac7af71fc95
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Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure

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