Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds

Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is...

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Autores principales:	I. A. Shurygina, V. A. Umanets, M. G. Shurygin
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	марк healing process adhesive process р38 cascade jnk cascade erk cascade Science Q
Acceso en línea:	https://doaj.org/article/4d3fecefbf2d4871ac9194b8ce9e9b1c
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spelling	oai:doaj.org-article:4d3fecefbf2d4871ac9194b8ce9e9b1c2021-11-23T06:14:43ZDynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds2541-94202587-959610.29413/ABS.2019-4.5.9https://doaj.org/article/4d3fecefbf2d4871ac9194b8ce9e9b1c2019-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2170https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day.I. A. ShuryginaV. A. UmanetsM. G. ShuryginScientific Сentre for Family Health and Human Reproduction Problemsarticleмаркhealing processadhesive processр38 cascadejnk cascadeerk cascadeScienceQRUActa Biomedica Scientifica, Vol 4, Iss 5, Pp 55-59 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	марк healing process adhesive process р38 cascade jnk cascade erk cascade Science Q
spellingShingle	марк healing process adhesive process р38 cascade jnk cascade erk cascade Science Q I. A. Shurygina V. A. Umanets M. G. Shurygin Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds
description	Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day.
format	article
author	I. A. Shurygina V. A. Umanets M. G. Shurygin
author_facet	I. A. Shurygina V. A. Umanets M. G. Shurygin
author_sort	I. A. Shurygina
title	Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds
title_short	Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds
title_full	Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds
title_fullStr	Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds
title_full_unstemmed	Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds
title_sort	dynamics of the activity of map-kinase cascades in the healing process of postoperative musculocutaneous wounds
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/4d3fecefbf2d4871ac9194b8ce9e9b1c
work_keys_str_mv	AT iashurygina dynamicsoftheactivityofmapkinasecascadesinthehealingprocessofpostoperativemusculocutaneouswounds AT vaumanets dynamicsoftheactivityofmapkinasecascadesinthehealingprocessofpostoperativemusculocutaneouswounds AT mgshurygin dynamicsoftheactivityofmapkinasecascadesinthehealingprocessofpostoperativemusculocutaneouswounds
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Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds

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