A hierarchical bilayer architecture for complex tissue regeneration

Engineering a complete, physiologically functional, periodontal complex structure remains a great clinical challenge due to the highly hierarchical architecture of the periodontium and coordinated regulation of multiple growth factors required to induce stem cell multilineage differentiation. Using...

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Autores principales:	Min Yu, Dan Luo, Jing Qiao, Jiusi Guo, Danqing He, Shanshan Jin, Lin Tang, Yu Wang, Xin Shi, Jing Mao, Shengjie Cui, Yu Fu, Zixin Li, Dawei Liu, Ting Zhang, Chi Zhang, Zhou Li, Yongsheng Zhou, Yan Liu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Biomimetic design Biphasic scaffold Mineralized collagen Micropatterned arrays Periodontium regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4d47ebdda4b74b549b759443929d3e47
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spelling	oai:doaj.org-article:4d47ebdda4b74b549b759443929d3e472021-11-26T04:36:52ZA hierarchical bilayer architecture for complex tissue regeneration2452-199X10.1016/j.bioactmat.2021.08.024https://doaj.org/article/4d47ebdda4b74b549b759443929d3e472022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X2100400Xhttps://doaj.org/toc/2452-199XEngineering a complete, physiologically functional, periodontal complex structure remains a great clinical challenge due to the highly hierarchical architecture of the periodontium and coordinated regulation of multiple growth factors required to induce stem cell multilineage differentiation. Using biomimetic self-assembly and microstamping techniques, we construct a hierarchical bilayer architecture consisting of intrafibrillarly mineralized collagen resembling bone and cementum, and unmineralized parallel-aligned fibrils mimicking periodontal ligament. The prepared biphasic scaffold possesses unique micro/nano structure, differential mechanical properties, and growth factor-rich microenvironment between the two phases, realizing a perfect simulation of natural periodontal hard/soft tissue interface. The interconnected porous hard compartment with a Young's modulus of 1409.00 ± 160.83 MPa could induce cross-arrangement and osteogenic differentiation of stem cells in vitro, whereas the micropatterned soft compartment with a Young's modulus of 42.62 ± 4.58 MPa containing abundant endogenous growth factors, could guide parallel arrangement and fibrogenic differentiation of stem cells in vitro. After implantation in critical-sized complete periodontal tissue defect, the biomimetic bilayer architecture potently reconstructs native periodontium with the insertion of periodontal ligament fibers into newly formed cementum and alveolar bone by recruiting host mesenchymal stem cells and activating the transforming growth factor beta 1/Smad3 signaling pathway. Taken together, integration of self-assembly and microstamping strategies could successfully fabricate a hierarchical bilayer architecture, which exhibits great potential for recruiting and regulating host stem cells to promote synergistic regeneration of hard/soft tissues.Min YuDan LuoJing QiaoJiusi GuoDanqing HeShanshan JinLin TangYu WangXin ShiJing MaoShengjie CuiYu FuZixin LiDawei LiuTing ZhangChi ZhangZhou LiYongsheng ZhouYan LiuKeAi Communications Co., Ltd.articleBiomimetic designBiphasic scaffoldMineralized collagenMicropatterned arraysPeriodontium regenerationMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 93-106 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biomimetic design Biphasic scaffold Mineralized collagen Micropatterned arrays Periodontium regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Biomimetic design Biphasic scaffold Mineralized collagen Micropatterned arrays Periodontium regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Min Yu Dan Luo Jing Qiao Jiusi Guo Danqing He Shanshan Jin Lin Tang Yu Wang Xin Shi Jing Mao Shengjie Cui Yu Fu Zixin Li Dawei Liu Ting Zhang Chi Zhang Zhou Li Yongsheng Zhou Yan Liu A hierarchical bilayer architecture for complex tissue regeneration
description	Engineering a complete, physiologically functional, periodontal complex structure remains a great clinical challenge due to the highly hierarchical architecture of the periodontium and coordinated regulation of multiple growth factors required to induce stem cell multilineage differentiation. Using biomimetic self-assembly and microstamping techniques, we construct a hierarchical bilayer architecture consisting of intrafibrillarly mineralized collagen resembling bone and cementum, and unmineralized parallel-aligned fibrils mimicking periodontal ligament. The prepared biphasic scaffold possesses unique micro/nano structure, differential mechanical properties, and growth factor-rich microenvironment between the two phases, realizing a perfect simulation of natural periodontal hard/soft tissue interface. The interconnected porous hard compartment with a Young's modulus of 1409.00 ± 160.83 MPa could induce cross-arrangement and osteogenic differentiation of stem cells in vitro, whereas the micropatterned soft compartment with a Young's modulus of 42.62 ± 4.58 MPa containing abundant endogenous growth factors, could guide parallel arrangement and fibrogenic differentiation of stem cells in vitro. After implantation in critical-sized complete periodontal tissue defect, the biomimetic bilayer architecture potently reconstructs native periodontium with the insertion of periodontal ligament fibers into newly formed cementum and alveolar bone by recruiting host mesenchymal stem cells and activating the transforming growth factor beta 1/Smad3 signaling pathway. Taken together, integration of self-assembly and microstamping strategies could successfully fabricate a hierarchical bilayer architecture, which exhibits great potential for recruiting and regulating host stem cells to promote synergistic regeneration of hard/soft tissues.
format	article
author	Min Yu Dan Luo Jing Qiao Jiusi Guo Danqing He Shanshan Jin Lin Tang Yu Wang Xin Shi Jing Mao Shengjie Cui Yu Fu Zixin Li Dawei Liu Ting Zhang Chi Zhang Zhou Li Yongsheng Zhou Yan Liu
author_facet	Min Yu Dan Luo Jing Qiao Jiusi Guo Danqing He Shanshan Jin Lin Tang Yu Wang Xin Shi Jing Mao Shengjie Cui Yu Fu Zixin Li Dawei Liu Ting Zhang Chi Zhang Zhou Li Yongsheng Zhou Yan Liu
author_sort	Min Yu
title	A hierarchical bilayer architecture for complex tissue regeneration
title_short	A hierarchical bilayer architecture for complex tissue regeneration
title_full	A hierarchical bilayer architecture for complex tissue regeneration
title_fullStr	A hierarchical bilayer architecture for complex tissue regeneration
title_full_unstemmed	A hierarchical bilayer architecture for complex tissue regeneration
title_sort	hierarchical bilayer architecture for complex tissue regeneration
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/4d47ebdda4b74b549b759443929d3e47
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_version_	1718409839729704960

A hierarchical bilayer architecture for complex tissue regeneration

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