Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus
Abstract Background Our recently reported doubled haploid (DH) induction lines e.g., Y3380 and Y3560 are allo-octoploid (AAAACCCC, 2n = 8× ≈ 76), which can induce the maternal parent to produce DH individuals. Whether this induction process is related to the production of aneuploid gametes form male...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
BMC
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/4d537c7a02ae419e847fa4e77e35f930 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:4d537c7a02ae419e847fa4e77e35f930 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:4d537c7a02ae419e847fa4e77e35f9302021-11-21T12:05:46ZGenetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus10.1186/s12870-021-03311-z1471-2229https://doaj.org/article/4d537c7a02ae419e847fa4e77e35f9302021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03311-zhttps://doaj.org/toc/1471-2229Abstract Background Our recently reported doubled haploid (DH) induction lines e.g., Y3380 and Y3560 are allo-octoploid (AAAACCCC, 2n = 8× ≈ 76), which can induce the maternal parent to produce DH individuals. Whether this induction process is related to the production of aneuploid gametes form male parent and genetic characteristics of the male parent has not been reported yet. Results Somatic chromosome counts of DH inducer parents, female wax-less parent (W1A) and their F1 hybrid individuals revealed the reliability of flow cytometry analysis. Y3560 has normal chromosome behavior in metaphase I and anaphase I, but chromosome division was not synchronized in the tetrad period. Individual phenotypic identification and flow cytometric fluorescence measurement of F1 individual and parents revealed that DH individuals can be distinguished on the basis of waxiness trait. The results of phenotypic identification and flow cytometry can identify the homozygotes or heterozygotes of F1 generation individuals. The data of SNP genotyping coupled with phenotypic waxiness trait revealed that the genetic distance between W1A and F1 homozygotes were smaller as compared to their heterozygotes. It was found that compared with allo-octoploids, aneuploidy from allo-octoploid segregation did not significantly increase the DH induction rate, but reduced male infiltration rate and heterozygous site rate of induced F1 generation. The ploidy, SNP genotyping and flow cytometry results cumulatively shows that DH induction is attributed to the key genes regulation from the parents of Y3560 and Y3380, which significantly increase the induction efficiency as compared to ploidy. Conclusion Based on our findings, we hypothesize that genetic characteristics and aneuploidy play an important role in the induction of DH individuals in Brassca napus, and the induction process has been explored. It provides an important insight for us to locate and clone the genes that regulate the inducibility in the later stage.Xuan LuoJin YangZhendong ZhuLiangjun HuangAsif AliHafiz Hassan JavedWei ZhangYing ZhouLiqin YinPeizhou XuXingyu LiangYun LiJisheng WangQiong ZouWanzhuo GongHaoran ShiLanrong TaoZeming KangRong TangHailan LiuShaohong FuBMCarticleDoubled haploid inducerFlow cytometryPhenotypic identificationSNP genotypingHomozygous sites rateAneuploidyBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-17 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Doubled haploid inducer Flow cytometry Phenotypic identification SNP genotyping Homozygous sites rate Aneuploidy Botany QK1-989 |
| spellingShingle |
Doubled haploid inducer Flow cytometry Phenotypic identification SNP genotyping Homozygous sites rate Aneuploidy Botany QK1-989 Xuan Luo Jin Yang Zhendong Zhu Liangjun Huang Asif Ali Hafiz Hassan Javed Wei Zhang Ying Zhou Liqin Yin Peizhou Xu Xingyu Liang Yun Li Jisheng Wang Qiong Zou Wanzhuo Gong Haoran Shi Lanrong Tao Zeming Kang Rong Tang Hailan Liu Shaohong Fu Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus |
| description |
Abstract Background Our recently reported doubled haploid (DH) induction lines e.g., Y3380 and Y3560 are allo-octoploid (AAAACCCC, 2n = 8× ≈ 76), which can induce the maternal parent to produce DH individuals. Whether this induction process is related to the production of aneuploid gametes form male parent and genetic characteristics of the male parent has not been reported yet. Results Somatic chromosome counts of DH inducer parents, female wax-less parent (W1A) and their F1 hybrid individuals revealed the reliability of flow cytometry analysis. Y3560 has normal chromosome behavior in metaphase I and anaphase I, but chromosome division was not synchronized in the tetrad period. Individual phenotypic identification and flow cytometric fluorescence measurement of F1 individual and parents revealed that DH individuals can be distinguished on the basis of waxiness trait. The results of phenotypic identification and flow cytometry can identify the homozygotes or heterozygotes of F1 generation individuals. The data of SNP genotyping coupled with phenotypic waxiness trait revealed that the genetic distance between W1A and F1 homozygotes were smaller as compared to their heterozygotes. It was found that compared with allo-octoploids, aneuploidy from allo-octoploid segregation did not significantly increase the DH induction rate, but reduced male infiltration rate and heterozygous site rate of induced F1 generation. The ploidy, SNP genotyping and flow cytometry results cumulatively shows that DH induction is attributed to the key genes regulation from the parents of Y3560 and Y3380, which significantly increase the induction efficiency as compared to ploidy. Conclusion Based on our findings, we hypothesize that genetic characteristics and aneuploidy play an important role in the induction of DH individuals in Brassca napus, and the induction process has been explored. It provides an important insight for us to locate and clone the genes that regulate the inducibility in the later stage. |
| format |
article |
| author |
Xuan Luo Jin Yang Zhendong Zhu Liangjun Huang Asif Ali Hafiz Hassan Javed Wei Zhang Ying Zhou Liqin Yin Peizhou Xu Xingyu Liang Yun Li Jisheng Wang Qiong Zou Wanzhuo Gong Haoran Shi Lanrong Tao Zeming Kang Rong Tang Hailan Liu Shaohong Fu |
| author_facet |
Xuan Luo Jin Yang Zhendong Zhu Liangjun Huang Asif Ali Hafiz Hassan Javed Wei Zhang Ying Zhou Liqin Yin Peizhou Xu Xingyu Liang Yun Li Jisheng Wang Qiong Zou Wanzhuo Gong Haoran Shi Lanrong Tao Zeming Kang Rong Tang Hailan Liu Shaohong Fu |
| author_sort |
Xuan Luo |
| title |
Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus |
| title_short |
Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus |
| title_full |
Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus |
| title_fullStr |
Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus |
| title_full_unstemmed |
Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus |
| title_sort |
genetic characteristics and ploidy trigger the high inducibility of double haploid (dh) inducer in brassica napus |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/4d537c7a02ae419e847fa4e77e35f930 |
| work_keys_str_mv |
AT xuanluo geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT jinyang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT zhendongzhu geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT liangjunhuang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT asifali geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT hafizhassanjaved geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT weizhang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT yingzhou geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT liqinyin geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT peizhouxu geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT xingyuliang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT yunli geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT jishengwang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT qiongzou geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT wanzhuogong geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT haoranshi geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT lanrongtao geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT zemingkang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT rongtang geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT hailanliu geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus AT shaohongfu geneticcharacteristicsandploidytriggerthehighinducibilityofdoublehaploiddhinducerinbrassicanapus |
| _version_ |
1718419243493490688 |