Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis

Abstract Detection of micropollutants such as steroid hormones occurring in the aquatic environment at concentrations between ng/L and µg/L remains a major challenge, in particular when treatment efficiency is to be evaluated. Steroid hormones are typically analysed using mass-spectrometry methods,...

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Autores principales:	Roman Lyubimenko, Bryce S. Richards, Andrey Turshatov, Andrea I. Schäfer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d59a744dc6f4410bf762f478419cdf6
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spelling	oai:doaj.org-article:4d59a744dc6f4410bf762f478419cdf62021-12-02T17:14:58ZSeparation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis10.1038/s41598-020-63697-y2045-2322https://doaj.org/article/4d59a744dc6f4410bf762f478419cdf62020-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-63697-yhttps://doaj.org/toc/2045-2322Abstract Detection of micropollutants such as steroid hormones occurring in the aquatic environment at concentrations between ng/L and µg/L remains a major challenge, in particular when treatment efficiency is to be evaluated. Steroid hormones are typically analysed using mass-spectrometry methods, requiring pre-concentration and/or derivatisation procedures to achieve required detection limits. Free of sample preparation steps, the use of radiolabelled contaminants with liquid scintillation counting is limited to single-compound systems and require a separation of hormone mixtures before detection. In this work, a method was developed coupling ultra-high-pressure liquid chromatography (UHPLC) with flow scintillation analysis (FSA) for separation and detection of radiolabelled estrone, 17ß-estradiol, testosterone and progesterone. Adjustment of the flow rate of scintillation liquid and UHPLC mobile phase, gradient time, column temperature, and injection volume allowed the separation of steroid hormones and degradation products. The limit-of-detection (LOD = 1.5–2.4 ng/L) and limit-of-quantification (LOQ = 3.4–4.3 ng/L) for steroid hormones were comparable with the current state-of-the-art technique (LC-MS/MS) for non-derivatised compounds. Although the method cannot be applied to real water samples (unless spiked with radiotracers), it serves as a useful tool for the development of water treatment technologies at laboratory scale as demonstrated via: i) adsorption on polymer-based spherical activated carbon, ii) retention in nanofiltration, iii) photodegradation using a photocatalytic membrane.Roman LyubimenkoBryce S. RichardsAndrey TurshatovAndrea I. SchäferNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roman Lyubimenko Bryce S. Richards Andrey Turshatov Andrea I. Schäfer Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
description	Abstract Detection of micropollutants such as steroid hormones occurring in the aquatic environment at concentrations between ng/L and µg/L remains a major challenge, in particular when treatment efficiency is to be evaluated. Steroid hormones are typically analysed using mass-spectrometry methods, requiring pre-concentration and/or derivatisation procedures to achieve required detection limits. Free of sample preparation steps, the use of radiolabelled contaminants with liquid scintillation counting is limited to single-compound systems and require a separation of hormone mixtures before detection. In this work, a method was developed coupling ultra-high-pressure liquid chromatography (UHPLC) with flow scintillation analysis (FSA) for separation and detection of radiolabelled estrone, 17ß-estradiol, testosterone and progesterone. Adjustment of the flow rate of scintillation liquid and UHPLC mobile phase, gradient time, column temperature, and injection volume allowed the separation of steroid hormones and degradation products. The limit-of-detection (LOD = 1.5–2.4 ng/L) and limit-of-quantification (LOQ = 3.4–4.3 ng/L) for steroid hormones were comparable with the current state-of-the-art technique (LC-MS/MS) for non-derivatised compounds. Although the method cannot be applied to real water samples (unless spiked with radiotracers), it serves as a useful tool for the development of water treatment technologies at laboratory scale as demonstrated via: i) adsorption on polymer-based spherical activated carbon, ii) retention in nanofiltration, iii) photodegradation using a photocatalytic membrane.
format	article
author	Roman Lyubimenko Bryce S. Richards Andrey Turshatov Andrea I. Schäfer
author_facet	Roman Lyubimenko Bryce S. Richards Andrey Turshatov Andrea I. Schäfer
author_sort	Roman Lyubimenko
title	Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
title_short	Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
title_full	Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
title_fullStr	Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
title_full_unstemmed	Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
title_sort	separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4d59a744dc6f4410bf762f478419cdf6
work_keys_str_mv	AT romanlyubimenko separationanddegradationdetectionofnanogramperlitreconcentrationsofradiolabelledsteroidhormonesusingcombinedliquidchromatographyandflowscintillationanalysis AT brycesrichards separationanddegradationdetectionofnanogramperlitreconcentrationsofradiolabelledsteroidhormonesusingcombinedliquidchromatographyandflowscintillationanalysis AT andreyturshatov separationanddegradationdetectionofnanogramperlitreconcentrationsofradiolabelledsteroidhormonesusingcombinedliquidchromatographyandflowscintillationanalysis AT andreaischafer separationanddegradationdetectionofnanogramperlitreconcentrationsofradiolabelledsteroidhormonesusingcombinedliquidchromatographyandflowscintillationanalysis
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Separation and degradation detection of nanogram-per-litre concentrations of radiolabelled steroid hormones using combined liquid chromatography and flow scintillation analysis

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