Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering

(1) Background: Vascularization remains a critical challenge in bone tissue engineering. The objective of this study was to prevascularize calcium phosphate cement (CPC) scaffold by co-culturing human periodontal ligament stem cells (hPDLSCs) and human umbilical vein endothelial cells (hUVECs) for t...

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Autores principales:	Zeqing Zhao, Yaxi Sun, Qingchen Qiao, Li Zhang, Xianju Xie, Michael D. Weir, Abraham Schneider, Hockin H. K. Xu, Ning Zhang, Ke Zhang, Yuxing Bai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	prevascularization calcium phosphate scaffold co-culture human periodontal ligament stem cells endothelial cells bone tissue engineering Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4d5b75543d214674868c0c4696ec9500
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spelling	oai:doaj.org-article:4d5b75543d214674868c0c4696ec95002021-11-25T17:55:43ZHuman Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering10.3390/ijms2222123631422-00671661-6596https://doaj.org/article/4d5b75543d214674868c0c4696ec95002021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12363https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067(1) Background: Vascularization remains a critical challenge in bone tissue engineering. The objective of this study was to prevascularize calcium phosphate cement (CPC) scaffold by co-culturing human periodontal ligament stem cells (hPDLSCs) and human umbilical vein endothelial cells (hUVECs) for the first time; (2) Methods: hPDLSCs and/or hUVECs were seeded on CPC scaffolds. Three groups were tested: (i) hUVEC group (hUVECs on CPC); (ii) hPDLSC group (hPDLSCs on CPC); (iii) co-culture group (hPDLSCs + hUVECs on CPC). Osteogenic differentiation, bone mineral synthesis, and microcapillary-like structures were evaluated; (3) Results: Angiogenic gene expressions of co-culture group were 6–9 fold those of monoculture. vWF expression of co-culture group was 3 times lower than hUVEC-monoculture group. Osteogenic expressions of co-culture group were 2–3 folds those of the hPDLSC-monoculture group. ALP activity and bone mineral synthesis of co-culture were much higher than hPDLSC-monoculture group. Co-culture group formed capillary-like structures at 14–21 days. Vessel length and junction numbers increased with time; (4) Conclusions: The hUVECs + hPDLSCs co-culture on CPC scaffold achieved excellent osteogenic and angiogenic capability in vitro for the first time, generating prevascularized networks. The hPDLSCs + hUVECs co-culture had much better osteogenesis and angiogenesis than monoculture. CPC scaffolds prevacularized via hPDLSCs + hUVECs are promising for dental, craniofacial, and orthopedic applications.Zeqing ZhaoYaxi SunQingchen QiaoLi ZhangXianju XieMichael D. WeirAbraham SchneiderHockin H. K. XuNing ZhangKe ZhangYuxing BaiMDPI AGarticleprevascularizationcalcium phosphate scaffoldco-culturehuman periodontal ligament stem cellsendothelial cellsbone tissue engineeringBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12363, p 12363 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	prevascularization calcium phosphate scaffold co-culture human periodontal ligament stem cells endothelial cells bone tissue engineering Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	prevascularization calcium phosphate scaffold co-culture human periodontal ligament stem cells endothelial cells bone tissue engineering Biology (General) QH301-705.5 Chemistry QD1-999 Zeqing Zhao Yaxi Sun Qingchen Qiao Li Zhang Xianju Xie Michael D. Weir Abraham Schneider Hockin H. K. Xu Ning Zhang Ke Zhang Yuxing Bai Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering
description	(1) Background: Vascularization remains a critical challenge in bone tissue engineering. The objective of this study was to prevascularize calcium phosphate cement (CPC) scaffold by co-culturing human periodontal ligament stem cells (hPDLSCs) and human umbilical vein endothelial cells (hUVECs) for the first time; (2) Methods: hPDLSCs and/or hUVECs were seeded on CPC scaffolds. Three groups were tested: (i) hUVEC group (hUVECs on CPC); (ii) hPDLSC group (hPDLSCs on CPC); (iii) co-culture group (hPDLSCs + hUVECs on CPC). Osteogenic differentiation, bone mineral synthesis, and microcapillary-like structures were evaluated; (3) Results: Angiogenic gene expressions of co-culture group were 6–9 fold those of monoculture. vWF expression of co-culture group was 3 times lower than hUVEC-monoculture group. Osteogenic expressions of co-culture group were 2–3 folds those of the hPDLSC-monoculture group. ALP activity and bone mineral synthesis of co-culture were much higher than hPDLSC-monoculture group. Co-culture group formed capillary-like structures at 14–21 days. Vessel length and junction numbers increased with time; (4) Conclusions: The hUVECs + hPDLSCs co-culture on CPC scaffold achieved excellent osteogenic and angiogenic capability in vitro for the first time, generating prevascularized networks. The hPDLSCs + hUVECs co-culture had much better osteogenesis and angiogenesis than monoculture. CPC scaffolds prevacularized via hPDLSCs + hUVECs are promising for dental, craniofacial, and orthopedic applications.
format	article
author	Zeqing Zhao Yaxi Sun Qingchen Qiao Li Zhang Xianju Xie Michael D. Weir Abraham Schneider Hockin H. K. Xu Ning Zhang Ke Zhang Yuxing Bai
author_facet	Zeqing Zhao Yaxi Sun Qingchen Qiao Li Zhang Xianju Xie Michael D. Weir Abraham Schneider Hockin H. K. Xu Ning Zhang Ke Zhang Yuxing Bai
author_sort	Zeqing Zhao
title	Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering
title_short	Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering
title_full	Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering
title_fullStr	Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering
title_full_unstemmed	Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering
title_sort	human periodontal ligament stem cell and umbilical vein endothelial cell co-culture to prevascularize scaffolds for angiogenic and osteogenic tissue engineering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4d5b75543d214674868c0c4696ec9500
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Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering

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